Serum indicators of free radical activity in idiopathic pulmonary fibrosis.

Abstract

Serum levels of free radical activity were measured in 37 patients with idiopathic pulmonary fibrosis (IPF) and 16 control subjects. Three assays used were (1) simultaneously measured levels of the 9,11-diene conjugate of linoleic acid and 9,12-linoleic acid expressed as a percent molar ratio (%MR), a measure of free-radical-mediated lipid peroxidation; (2) thiobarbituric acid reactive substances (TBARS), one of which is malondialdehyde; (3) desferrioxamine-chelatable iron assay, a measure of the potential iron available to catalyze free radical generation. Mean %MR, TBARS and desferrioxamine-chelatable iron were all elevated initially in patients with IPF compared with control subjects (%MR, p < 0.0001; TBARS, p = 0.0013; desferrioxamine-chelatable iron, p = 0.0029). Furthermore, the serum %MR was higher in a subset of patients with clinically worsening IPF than in those patients with clinically stable disease (p = 0.002). Treatment did not appear to affect the three different serum indicators of free radical activity. Thus, lipid peroxidation appears to be increased in patients with IPF and is associated with an increase in desferrioxamine-chelatable iron levels. Serum % MR levels appeared to correlate with clinical disease activity, and they may have a role in monitoring disease activity.

Pharmacologic therapies for idiopathic pulmonary fibrosis, past and future

“…Its antioxidant properties have also made it an attractive option for the treatment of patients with IPF, since the disease process has been associated with excessive oxidative stress (16),(17). NAC augments the synthesis of glutathione in vivo (18), replenishing stores and restoring the epithelial cell antioxidant defense mechanism.…”

Serum biomarkers in idiopathic pulmonary fibrosis

“…Indeed, the oxidant burden in the lungs of patients with pulmonary fibrosis was found to be increased [65][66] and increased levels of H 2 O 2 and 8-isoprostane were found in the exhaled breath condensate of IPF patients [67]. Furthermore, in animal models for pulmonary fibrosis defective anti-oxidant mechanisms resulted in augmented fibrosis [16].…”

Change in serum marker of oxidative stress in the progression of idiopathic pulmonary fibrosis

“…They also showed that increased peroxidase activity was involved in the epithelial injury in IPF [7]. Jackson et al reported that free radical activity was increased based on the change in serum markers [8], while Rahman et al showed an imbalance between oxidative stress and anti-oxidative potency in serum and BALF in IPF patients [9].…”