Serum levels of matrix metalloproteinase-2 and -9 and conventional tumor markers (CEA and CA 19-9) in patients with colorectal and gastric cancers

Abstract

Background: Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, play an important role in tumor invasion and metastasis. This study aimed to determine the serum levels of MMP-2, MMP-9, 130-and 225-kDa gelatinolytic bands and conventional tumor markers, carcinoembryonic antigen (CEA) and cancer antigen (CA) 19-9, in patients with gastrointestinal cancers. The relationship between these parameters and clinicopathological factors was also studied. Methods: Sera from controls (ns19), and patients with colorectal (ns47) and gastric (ns34) cancer were collected prospectively. The gelatinolytic activities of MMP-2, MMP-9, 130-and 225-kDa bands were determined using gelatin zymography. CEA and CA 19-9 were determined using immunoradiometric assay (IRMA). Results: Serum levels of MMP-9, 130-and 225-kDa gelatinolytic bands, CEA, and CA 19-9, but not MMP-2, in colorectal and gastric cancer were significantly higher than that of controls. No significant correlation was found between histological grade or clinical stage and levels of MMP-9, 130-and 225-kDa gelatinolytic bands, which were correlated (rs0.61-0.89, p-0.005). Conclusions: Our findings suggest that zymographic determination of

Blood Tests for Early Detection of Colorectal Cancer

“…In a recent prospective study assessing MMP-2 and MMP-9 activity in serum samples of patients with CRC, those with gastric cancer, and controls, serum levels of MMP-9, but not MMP-2, were significantly higher in patients with CRC and gastric cancer than in controls [11]. By contrast, another study did not find any association between MMP-9 serum levels and the presence of CRC [12].…”

Laboratory medicine and medical oncology: the tale of two Cinderellas

“…Some data indicate that high circulating VEGF concentrations may predict the response to anti-VEGF therapy, e.g., bevacizumab [56] . Similarly, the determination of matrix metaloproteinases, enzymes that play an important role in tumor invasion and formation of metastases has been introduced [57] .…”

Metastasis-associated Factors Facilitating the Progression of Colorectal Cancer

“…Overexpression of the glycoprotein leads to inhibition of cell differentiation and disruption of cellular polarisation and tissue structure. It was observed that anoikis, an apoptotic process initiated when there is poor or no cell-ECM contact, is also downregulated by CEA overexpression (Lankiewicz et al, 2008;Emara et al, 2009).…”