Serum levels of transforming growth factor-α in patients after partial hepatectomy as determined with an enzyme-linked immunosorbent assay

Tomiya, Tomoaki; Fujiwara, Michio

doi:10.1002/hep.1840180213

Cited by 34 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[14][15][16][17] During liver regeneration, TGF-␣ levels increase in liver tissue in rats and in serum in humans. 16,18,19 Moreover, these growth factors and their receptor (EGFr) have been described to determine tumor behavior in human colorectal cancer cells in vitro and in vivo. [20][21][22][23] Therefore, we and others hypothesized that growth factors responsible for liver regeneration could also affect tumor growth of clinically undetectable liver metastases in the remnant liver after partial liver resection.…”

Section: Discussionmentioning

confidence: 99%

Clinical relevance of transforming growth factor α, epidermal growth factor receptor, p53, and Ki67 in colorectal liver metastases and corresponding primary tumors

et al. 1998

View full text Add to dashboard Cite

To determine whether the expression of transforming growth factor ␣ (TGF-␣), its receptor (epidermal growth factor receptor [EGFr]), p53 nuclear protein, and proliferation influences prognosis of patients with liver metastases, a study was performed in 45 liver metastases and 33 corresponding primary colorectal carcinomas in patients referred for liver surgery. The expression of TGF-␣, EGFr, p53 nuclear protein, and proliferation rate was correlated with clinicopathological characteristics and survival after partial liver resection. In liver metastases, TGF-␣ expression was low in 42%, intermediate in 35%, and high in 23%. TGF-␣ expression was higher in liver metastases derived from lymph node-positive primary carcinomas, in synchronous and in irresectable liver metastases compared with those derived from lymph node-negative primary carcinomas, metachronous, and resectable liver metastases. Nuclear p53 expression was found in 83% of primary tumors and 71% of liver metastases. p53 expression did not correlate with the various clinicopathological characteristics. Ki67 expression was not associated with clinicopathological characteristics in primary and metastatic tumors. In the 38 patients in whom a partial liver resection was performed, median survival was 25 months in patients with a higher TGF-␣ expression in the metastasis than in the primary tumor and 60 months in patients with comparable or lower TGF-␣ expression in the metastasis than in the primary tumor (P ‫؍‬ .036). Median survival after liver resection was 21 months in patients with p53-negative liver metastases and 58 months in patients with p53-positive metastases (P ‫؍‬ .043). By multivariate analysis, p53 and EGFr expression on liver metastases were the best predictors of diseasefree survival after partial liver resection, with relative risks of 2.38 and 3.33, respectively. In patients with colorectal liver metastases, referred for liver surgery, a higher TGF-␣ expression is associated with unfavorable tumor characteristics, whereas p53 and absence of EGFr expression is associated with a better survival after partial liver resection. (HEPATOLOGY 1998;28:971-979.)Colorectal carcinoma is the second-leading cause of cancer deaths in the Western community. Of all colorectal cancer patients, about 50% will die within 5 years after diagnosis. 1 At the time of initial diagnosis, 15% to 25% of patients present with synchronous liver metastases. 1 It is estimated that overall, only 5% of patients who develop liver metastases of colorectal carcinomas are amenable to partial liver resection. 2 Despite intentionally curative liver resections, only 20% to 30% of these patients have a disease-free 5-year survival. [3][4][5] Furthermore, in patients with recurrences after liver resection, these recurrences often appear early after liver surgery, with the liver being a frequent site of initial failure. 5,6 In the animal model, tumor growth can be influenced by growth factors produced by the tumor cells and by growth factors generated by the organ that harbors the m...

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical relevance of transforming growth factor α, epidermal growth factor receptor, p53, and Ki67 in colorectal liver metastases and corresponding primary tumors

et al. 1998

View full text Add to dashboard Cite

show abstract

“…All these receptors contain a tyrosine kinase domain in their intracellular portion that autophosphorylates the receptor for activation. The TNF effect may be intracellular on receptor tyrosine kinase or extracellular through cross-talk between TNF and [52][53][54] showed that the level of TGF-␣ in blood may be a good indicator of liver growth and correlates better than HGF levels with the extent of the regenerative response. However, it is not known whether growth factor production in LDLT is adequate for optimal growth of the donor and recipient livers.…”

Section: Basic Mechanisms Of Liver Regeneration After Phmentioning

confidence: 99%

Liver regeneration: From laboratory to clinic

Fausto

2001

Liver Transplantation

127

114

View full text Add to dashboard Cite

“…Several growth factors for hepatocytes such as HGF, EGF, TGF-α and heparin-binding EGF-like growth factor (HB-EGF) have been reported and characterized [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. They were named hepatocyte proliferation factor-1 (HPF-1) and hepatocyte proliferation factor-2 (HPF-2).…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte proliferation factors from neonatal pig liver: Purification and characterization

et al. 2002

View full text Add to dashboard Cite

Two factors were found in the condition medium of neonatal pig liver fragments, which were capable of stimulating DNA synthesis in primary hepatocytes. They were named hepatocyte proliferation factor (HPF)-1 and HPF-2 and purified 1,025- and 2,580-fold, respectively. Both HPF-1 and HPF-2 seem to be anionic at pH 8.0 judged from the elution pattern of DEAE (DE52) column chromatography. HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Purified HPF-1 stimulated DNA synthesis of primary rat hepatocytes, but suppressed that of HepG2 cells. HPF-2 strongly bound to blue Sepharose and heparin Sepharose columns, and had a molecular weight of 71-90 kDa as estimated by SDS-PAGE under non-reduced condition. Purified HPF-2 stimulated DNA synthesis of primary rat hepatocytes dose dependently but did not suppress that of HepG2 cells. From further biological and chemical characteristics studied in this paper, HPF-1 and HPF-2 may be novel stimulating proteins for hepatocyte proliferation, although the possibility that they are already known growth factors can not be excluded without complete purification and its cloning.

show abstract

Serum levels of transforming growth factor-α in patients after partial hepatectomy as determined with an enzyme-linked immunosorbent assay

Cited by 34 publications

References 27 publications

Clinical relevance of transforming growth factor α, epidermal growth factor receptor, p53, and Ki67 in colorectal liver metastases and corresponding primary tumors

Clinical relevance of transforming growth factor α, epidermal growth factor receptor, p53, and Ki67 in colorectal liver metastases and corresponding primary tumors

Liver regeneration: From laboratory to clinic

Hepatocyte proliferation factors from neonatal pig liver: Purification and characterization

Contact Info

Product

Resources

About