Serum lipids and lipid peroxidation pattern in industrial and rural workers in India

Reddy, K. Krishna; Bulliyya, G.; Chandraiah, T.; Kumari, K. Nalini; Reddanna, Pallu; Thyagaraju, K.

doi:10.1007/bf02434835

Cited by 10 publications

(1 citation statement)

References 18 publications

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“…Lipid peroxidation and toxicity associated with oxygen radicals have been suggested as major causes of cancer, atherosclerosis and the aging process (Reddy et al, 1991). The damage of endothelial cells may lead to cardiovascular and cerebrovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

Protection of vascular endothelial cells from hydrogen peroxide‐induced oxidant injury by gypenosides, saponins of Gynostemma pentaphyllum

Lin

Lau

1993

Phytotherapy Research

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Lipid peroxidation and toxicity associated with oxygen radicals have been suggested as major causes of cancer, atherosclerosis and the aging process. Damage of endothelial cells may lead to cardiovascular and cerebrovascular diseases. Endothelial cells are susceptible to oxidant insult. In the present study, the antioxidant effect of a Chinese medicinal herb, Gynostemma pentaphyllum Makino (Chinese name, Jiaogulan), was investigated in uitro using vascular endothelial cells. Confluent monolayers of bovine pulmonary artery endothelial cells (PAEC) were preincubated with different concentrations of gypenosides (GP, total saponins of Gynostemma pentaphyllum) for 16 h, then washed and incubated with hydrogen peroxide ( H P 3 for 4 h. Cell injury was assessed by measuring the release of intracellular lactate dehydrogenase (LDH), and cell viability with tetrazolium (MTT) assay. Lipid peroxidation products of PAEC were monitored as thiobarbituric acid-reactive substances (TBARS) with a fluorometric assay. The results showed that 62.5 p~ HzOz incubated with PAEC for 4 h increased the percentage of LDH release, decreased cell viability manifested by MTT absorbance at 620 nm, and elevated TBARS. Preincubation of GP (25-150 pg/mL) with PAEC for 16 h before HzOz exposure significantly declined LDH release, increased cell viability, and reduced TBARS. These results demonstrate that gypenosides can protect vascular endothelial cells from oxidant injury. The data thus suggest that gypenosides may be beneficial for the prevention and treatment of atherosclerosis and for retardation of the aging process.

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Section: Introductionmentioning

confidence: 99%

Protection of vascular endothelial cells from hydrogen peroxide‐induced oxidant injury by gypenosides, saponins of Gynostemma pentaphyllum

Lin

Lau

1993

Phytotherapy Research

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Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells

Geng

Lau

1997

Phytother. Res.

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Garlic compounds protect vascular endothelial cells from hydrogen peroxide‐induced oxidant injury

Yamasaki

Lin

Lau

1994

Phytotherapy Research

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Oxygen radical injury and lipid peroxidation have been suggested as major causes of cancer, atherosclerosis and the aging process. In this study, we determined in uitro the effect of aged garlic extract (AGE) and one of its components, S-ally1 cysteine (SAC), on hydrogen peroxide (HP1)-induced oxidant injury using bovine pulmonary artery endothelial cells (PAEC). After overnight preincubation with AGE or SAC, PAEC monolayers were exposed to H202 for 3 h. Cell viability, lactate dehydrogenase (LDH) release and lipid peroxidation were measured to assess oxidant injury. Pretreatment with AGE at 2-4 mglmL or SAC at 4 mg/mL significantly reversed the loss of cell viability induced by 50 and 100 p m of H&. AGE or SAC also exhibited a dose-dependent inhibition of both LDH release and lipid peroxidation induced by 50 ~L M of H202. The results show that both AGE and SAC can protect vascular endothelial cells from oxidant injury. The data thus suggest that these compounds may be useful for retardation of the aging process and for prevention of cancer and atherosclerosis

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Serum lipids and lipid peroxidation pattern in industrial and rural workers in India

Cited by 10 publications

References 18 publications

Protection of vascular endothelial cells from hydrogen peroxide‐induced oxidant injury by gypenosides, saponins of Gynostemma pentaphyllum

Protection of vascular endothelial cells from hydrogen peroxide‐induced oxidant injury by gypenosides, saponins of Gynostemma pentaphyllum

Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells

Garlic compounds protect vascular endothelial cells from hydrogen peroxide‐induced oxidant injury

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