Serum lipoproteins, antioxidants and urine biochemical constituents in camel cystitis

Ellah, Mahmoud R. Abd; Khamis, Gaber F.; El-Nisr, Neveen A.

doi:10.1007/s00580-010-1121-3

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…In dromedary camels with a normal periparturient period, it was also reported that the levels of MDA did not differ significantly between the prepartum and postpartum periods. However, it was reported that MDA levels were increased in a variety of inflammatory conditions like acute and chronic cystitis in camels (Abd Ellah et al 2012), and urinary tract infection in human patients (Kurutas et al 2005). The other OS biomarker GSH however was lower in diseased groups compared to controls.…”

Section: Discussionmentioning

confidence: 95%

Impact of Intestinal and Urinary Tracts Obstruction on Oxidative Stress Biomarkers in Dromedary Camels

2023

Int J Vet Sci

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The purpose of this study was to evaluate the status of the oxidative stress biomarkers in camels with obstructions of the gastrointestinal and urinary tracts. Eighteen dromedary camels were examined at the Qassim University Veterinary Hospital in Saudi Arabia because of obstructions in the gastrointestinal (n=13) or urinary tracts (n=5). Camels were referred for evaluation because of anorexia, absence of defecation, abdominal distension, anuria and urine dribbling. Ten clinically healthy female dromedary camels were enrolled in this study as controls. A 7mL blood sample was collected in plain tubes from each camel for serum harvesting. Stress biomarkers including malondialdehyde (MDA), catalase (CAT), Glutathione (GSH), and superoxide dismutase (SOD) were determined in the sera. Parallel, lipid profiles including cholesterol, triglycerides (TG), high-density lipoproteins (HDL) and low-density lipoproteins (LDL) were also measured. Results showed that the MDA values did not differ significantly among camels with obstructions in the gastrointestinal and urinary tracts. However, the GSH value was significantly lower in camels with intestinal obstruction compared to control. In a similar manner, the SOD value was also significantly lower in animals with intestinal obstruction compared to the healthy ones, with a statistically significant difference. Both GSH and SOD values did not differ significantly between camels with urinary obstruction and control group. The value of CAT in the controls did not differ significantly from the values in both diseased groups. Data of lipid profile analysis showed that the cholesterol and TG values were higher in the diseased groups compared to the healthy group. The HDL value was lower in the group of intestinal obstruction compared to the healthy animals, with a statistically significant difference. However, on the contrary, the LDL value was higher in the group of intestinal obstruction compared to the healthy animals, with a statistically significant. Both HDL and LDL values did not differ significantly between camels with urinary obstruction and control group.

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Section: Discussionmentioning

confidence: 95%

Impact of Intestinal and Urinary Tracts Obstruction on Oxidative Stress Biomarkers in Dromedary Camels

2023

Int J Vet Sci

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“…Data described in this study provide a reliable biochemical evidence for the generation of circulating oxidative stress as detected by enhanced lipid peroxidation (sMDA and eMDA) and decreased serum levels of the enzymatic (SOD, CAT) and non-enzymatic (GSH) antioxidant markers in dromedary camels suffering UTI. It was reported that MDA levels were increased in a varieties of inflammatory conditions like acute and chronic cystitis in camels ( Abd Ellah, Khamis & Elnisr, 2012 ), UTI in human patients ( Kurutas et al, 2005 ), liver abscess in camels ( El-Deeb & Fouda, 2013 ), mastitis in does ( El-Deeb, 2013 ) pneumonia in calves ( El-Bahr & EL-Deeb, 2013 ). In addition, levels of lipid hydroperoxide were increased in erythrocytes isolated from dairy cows with acute mastitis ( Castillo et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

The diagnostic and prognostic importance of oxidative stress biomarkers and acute phase proteins in Urinary Tract Infection (UTI) in camels

El-Deeb

Buczinski

2015

PeerJ

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The present study aimed to investigate the diagnostic and prognostic importance of oxidative stress biomarkers and acute phase proteins in urinary tract infection (UTI) in camels. We describe the clinical, bacteriological and biochemical findings in 89 camels. Blood and urine samples from diseased (n = 74) and control camels (n = 15) were submitted to laboratory investigations. The urine analysis revealed high number of RBCS and pus cells. The concentrations of serum and erythrocytic malondialdehyde (sMDA & eMDA), Haptoglobin (Hp), serum amyloid A (SAA), Ceruloplasmin (Cp), fibrinogen (Fb), albumin, globulin and interleukin 6 (IL-6) were higher in diseased camels when compared to healthy ones. Catalase, super oxide dismutase and glutathione levels were lower in diseased camels when compared with control group. Forty one of 74 camels with UTI were successfully treated. The levels of malondialdehyde, catalase, super oxide dismutase, glutathione, Hp, SAA, Fb, total protein, globulin and IL-6 were associated with the odds of treatment failure. The MDA showed a great sensitivity (Se) and specificity (Sp) in predicting treatment failure (Se 85%/Sp 100%) as well as the SAA (Se 92%/Sp 87%) and globulin levels (Se 85%/Sp 100%) when using the cutoffs that maximizes the sum of Se + Sp. Multivariate logistic regression analysis revealed that two models had a high accuracy to predict failure with the first model including sex, sMDA and Hp as covariates (area under the receiver operating characteristic curve (AUC) = 0.92) and a second model using sex, SAA and Hp (AUC = 0.89). Conclusively, the oxidative stress biomarkers and acute phase proteins could be used as diagnostic and prognostic biomarkers in camel UTI management. Efforts should be forced to investigate such biomarkers in other species with UTI.

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“…Our results showed the capability of the different treatments to reduce the urinary MDA levels after its elevation during the infection. MDA levels were reported to increase in pregnant women with UTIs 95 during acute and chronic cystitis in camels 96 , and in diabetic patients with E. coli UTI 85 . In rats exposed to d-gal-induced testicular oxidative stress 97 .…”

Section: Microbiological Evaluationmentioning

confidence: 97%

Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model

Abdelgalil,

Yassin,

Khattab

et al. 2024

Sci Rep

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One of the most prevalent disorders of the urinary system is urinary tract infection, which is mostly brought on by uropathogenic Escherichia coli (UPEC). The objective of this study was to evaluate the regenerative therapeutic and antibacterial efficacy of PRP for induced bacterial cystitis in dogs in comparison to conventional antibiotics. 25 healthy male mongrel dogs were divided into 5 groups (n = 5). Control negative group that received neither induced infection nor treatments. 20 dogs were randomized into 4 groups after two weeks of induction of UPEC cystitis into; Group 1 (control positive; G1) received weekly intravesicular instillation of sodium chloride 0.9%. Group 2 (syst/PRP; G2), treated with both systemic intramuscular antibiotic and weekly intravesicular instillation of PRP; Group 3 (PRP; G3), treated with weekly intravesicular instillation of PRP, and Group 4 (syst; G4) treated with an intramuscular systemic antibiotic. Animals were subjected to weekly clinical, ultrasonographic evaluation, urinary microbiological analysis, and redox status biomarkers estimation. Urinary matrix metalloproteinases (MMP-2, MMP-9) and urinary gene expression for platelet-derived growth factor -B (PDGF-B), nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) were measured. At the end of the study, dogs were euthanized, and the bladder tissues were examined macroscopically, histologically, and immunohistochemically for NF-κB P65 and Cox-2. The PRP-treated group showed significant improvement for all the clinical, Doppler parameters, and the urinary redox status (p < 0.05). The urinary MMPs activity was significantly decreased in the PRP-treated group and the expression level of urinary NGF and VEGF were downregulated while PDGFB was significantly upregulated (p < 0.05). Meanwhile, the urinary viable cell count was significantly reduced in all treatments (P < 0.05). Gross examination of bladder tissue showed marked improvement for the PRP-treated group, expressed in the histopathological findings. Immunohistochemical analysis revealed a marked increase in Cox-2 and NF-κB P65 in the PRP-treated group (P < 0.05). autologous CaCl2-activated PRP was able to overcome the bacterial infection, generating an inflammatory environment to overcome the old one and initiate tissue healing. Hence, PRP is a promising alternative therapeutic for UPEC cystitis instead of conventional antibiotics.

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Serum lipoproteins, antioxidants and urine biochemical constituents in camel cystitis

Cited by 3 publications

References 3 publications

Impact of Intestinal and Urinary Tracts Obstruction on Oxidative Stress Biomarkers in Dromedary Camels

Impact of Intestinal and Urinary Tracts Obstruction on Oxidative Stress Biomarkers in Dromedary Camels

The diagnostic and prognostic importance of oxidative stress biomarkers and acute phase proteins in Urinary Tract Infection (UTI) in camels

Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model

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