Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction

Chen, Xiao Li; Yang, Xiaoyi; Wang, N D; Ding, Chunhua; Yang, Yuejin; You, Zhebin; Q, Su; Chen, J H

doi:10.1080/00365510310003265

Cited by 63 publications

(48 citation statements)

References 11 publications

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“…24 Additionally, LPA also plays an important role in the occurrence and development of heart and cerebrovascular disease and kidney disease. 25,26 Our study found that the levels of MCP-1, Cr, and BUN and Paller's score were much lower in LPA treatment group than in 4 h I/R group (p < 0.01), indicating that LPA can reduce renal damage and protect renal function. The likely mechanism is that during renal I/R injury, LPA can inhibit the body to produce a variety of chemotactic factors, which prevent mononuclear macrophage infiltration into renal tissue, and meanwhile LPA also plays a role in antiapoptosis.…”

Section: Discussionmentioning

confidence: 61%

Lysophosphatidic Acid and Lovastatin Might Protect Kidney in Renal I/R Injury by Downregulating MCP-1 in Rat

et al. 2011

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Renal ischemia/reperfusion (I/R) injury is a major cause of renal failure. The aim of our study is to explore the role of lysophosphatidic acid (LPA) and lovastatin on renal I/R injury and its mechanism in the rat. Male Wistar rats were randomly divided into sham-operated group; renal I/R for 0 h, 4 h, 12 h, and 24 h groups; LPA treatment group; and lovastatin treatment group (n = 10). Rats were killed to determine the level of monocyte chemotactic protein-1 (MCP-1) in renal tissue, renal function [serum creatinine (Cr) and blood urea nitrogen (BUN)], and renal histomorphology to evaluate the effectiveness of LPA and lovastatin. Normal renal tissue had a low level of MCP-1. The level of MCP-1 began to rise at 0 h after reperfusion, reached peak value at 4 h, and then gradually fell off. Compared with sham-operated group, MCP-1 was increased in all renal I/R injury groups (p < 0.01). With the extension of reperfusion, Cr and BUN were significantly increased (p < 0.01). There were damages in kidney tubules, renal interstitium, and kidney glomerulus in renal I/R injury groups. Paller's score was significantly increased in all renal I/R injury groups compared with sham-operated group (p < 0.01). LPA and lovastatin reduced the level of MCP-1, Cr, BUN, and damages of renal histomorphology (p < 0.01). The level of MCP-1 in renal tissue dynamically increases in renal I/R injury, indicating that MCP-1 is involved in renal I/R injury. LPA and lovastatin might protect renal function by downregulating MCP-1 in renal I/R injury.

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Section: Discussionmentioning

confidence: 61%

Lysophosphatidic Acid and Lovastatin Might Protect Kidney in Renal I/R Injury by Downregulating MCP-1 in Rat

et al. 2011

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“…Immunogold has been applied for immunological detection for a long time, and its advantages include short detection time, low cost, and no requirement for special instruments [13][14][15][16][17] . We have compared our microarray with ELISA according to these aspects: (1) detection with microarray for 96 samples only takes 100 min, whereas the ELISA method takes 2.5-3.5 h; (2) preparing a microarray only needs 10-20 ng of antigen, whereas ELISA needs 100-200 ng, and (3) identification of microarray results does not need any special instruments, whereas the ELI-SA method needs an enzyme immune analyzer.…”

Section: Discussionmentioning

confidence: 99%

A Simple Parallel Analytical Method of Prenatal Screening

Li¹,

Yang²,

Zhang³

et al. 2006

Gynecol Obstet Invest

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Protein microarray has progressed rapidly in the past few years, but it is still hard to popularize it in many developing countries or small hospitals owing to the technical expertise required in practice. We developed a cheap and easy-to-use protein microarray based on dot immunogold filtration assay for parallel analysis of ToRCH-related antibodies including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus type 1 and 2 in sera of pregnant women. It does not require any expensive instruments and the assay results can be clearly recognized by the naked eye. We analyzed 186 random sera of outpatients at the gynecological department with our microarray and commercial ELISA kit, and the results showed there was no significant difference between the two detection methods. Validated by clinical application, the microarray is easy to use and has a unique advantage in cost and time. It is more suitable for mass prenatal screening or epidemiological screening than the ELISA format.

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“…It was reported that the serum LPA level in multiple myeloma patients was significantly higher than in normal controls (22), while it is increased in patients with acute myocardial infarction (23). The usage of serum samples, however, may not be suitable; the reported LPA values must have included LPA produced in vitro during sample preparation.…”

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confidence: 99%