Serum lysozyme as a marker of host resistance. I. Production by macrophages resident in rat sarcomata

Currie, G. A.; Eccles, Suzanne A.

doi:10.1038/bjc.1976.6

Cited by 43 publications

(9 citation statements)

References 7 publications

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“…Lysozyme, in the absence of overt granulocyte infiltration (noted histologically) is a constitutive marker for cells of the monocyte-macrophage series, and we interpret the high levels of this enzyme in tumour chamber fluids as reflecting macrophage infiltration. Serum levels of lysozyme in the tumourbearing animals were modestly elevated, as previously described (Currie & Eccles, 1976). In-vitro examination of tumour cells and macrophages indicates that the high levels of arginase in the tumour extracellular fluids are derived from macrophages resident within the tumour.…”

Section: Discussionsupporting

confidence: 77%

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Microenvironmental arginine depletion by macrophages in vivo

Currie¹,

Gyure²,

Cifuentes³

1979

Br J Cancer

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Summary.-Since the tumour-selective cytotoxic activity of activated macrophages in vitro can be attributed to depletion of the culture medium of L-arginine by macrophage arginase, a series of experiments was designed to determine whether such a mechanism could operate in vivo.Extracellular fluid obtained from Gullino chambers within established tumours contained high levels of arginase, no detectable arginine and high levels of ornithine. When tumours were disaggregated into single-cell suspensions, arginase was readily detected within tumour macrophages but not within malignant cells. Inflammatory ascites induced in mice by Corynebacterium parvum was rich in arginase, depleted of L-arginine and cytotoxic in vitro to L5178Y and V79 cells. High levels of arginase in the ascites fluid were associated with resistance to challenge with syngeneic L5178Y cells.Lymph collected from the cisterna chyli in rats bearing a macrophage-rich sarcoma on the small bowel contained elevated levels of arginase, was depleted of arginine and contained increased concentrations of ornithine.We conclude that in sites of macrophage infiltration there is microenvironmental arginine depletion due to the action of arginase, and that arginase release could represent an important macrophage effector mechanism against a variety of targets, including malignant cells, virus-infected cells, fungi and parasites.

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Section: Discussionsupporting

confidence: 77%

“…Arginase is present in high concentrations (i.e., higher than the surrounding normal tissues) in animnal tumours amul in granulomas (Edlbacher & Merz, 1927). In studies of skin carcinogenesis, Roberts & Frankel (1949) showed that tumours contain more arginase than normal skin.…”

Section: Discussionmentioning

confidence: 99%

Microenvironmental arginine depletion by macrophages in vivo

Currie¹,

Gyure²,

Cifuentes³

1979

Br J Cancer

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“…The demonstrated changed immunoglobulin levels might point to concommitant bacterial infection in patients with cervical cancer at the tumor site. However serum lysozyme which is considered as a parameter for macrophage activity has been found unchanged and almost in the normal range in all investigated cancer patients [23], Changes of immunoglobulin levels might be also a reaction to viral infection. An association between infection with herpes simplex virus type II and cervical cancer has been demonstrated in epidemiological [24] and immunological studies [25].…”

Section: Discussionmentioning

confidence: 80%

Investigations on General Immune Reactivity in Untreated Cervical Cancer Patients

Micksche¹,

Luger²,

Michalica³

et al. 1978

Oncology

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74 patients with untreated cervical cancer (FIGO II and III) were skintested with a battery of recall antigens, and also sensitised and challenged with DNCB. A significant reduction of reaction to Tuberkulin, Varidase and also to DNCB was found in patients with stage III in comparison to healthy females of the same age group. Significant changes in immunoglobulin levels, increase of IgA and decrease of IgG were ovserved in the cancer patients. Serum lysozyme values were the same as in the control group.

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“…One simple approach to achieve this goal is by incorporating lysozyme in RB chitosan adhesive films. Lysozyme concentrations fluctuate largely in the body, from 1 mg/mL (Temel et al, 1991) in tears to 10 g/mL in serum (Currie & Eccles, 1976) and 1 g/mL or lower in cerebrospinal fluid (Mishra, Batra, Ali, Anupurba, & Das, 2003). In our in vivo study, we tested adhesives with the lysozyme concentration found in tears (∼1 mg/mL) to ensure significant depolymerization, remaining nonetheless in physiological limits.…”

Section: Discussionmentioning

confidence: 99%