Serum metabolomics profiling and potential biomarkers of myopia using LC-QTOF/MS

Dai, Lili; Yang, Wanchao; Qin, Xu‐Jie; Liu, Ying; Cao, Hong-Ling; Zhou, Cong; Wang, Yuezhen

doi:10.1016/j.exer.2019.107737

Cited by 32 publications

(24 citation statements)

References 39 publications

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“…Oxidative stress, inflammation, and apoptosis may be key factors in the myopic regulatory pathways [ 3 – 6 ]. The low levels of 5-MTHF in myopia patients may lead to the increase of homocysteine, which closely correlated with oxidative stress, inflammation, and cellular apoptosis [ 5 ]. Furthermore, exogenous bFGF effectively ameliorates the excessive axial elongation in chronic form-deprivation myopia in chicks by suppressing retinal neuron apoptosis [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Orthokeratology and Low-Intensity Laser Therapy for Slowing the Progression of Myopia in Children

Xiong

Mao

Liao

et al. 2021

BioMed Research International

107

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Orthokeratology (OK) is widely used to slow the progression of myopia. Low-level laser therapy (LLLT) provides sufficient low energy to change the cellular function. This research is aimed at verifying the hypothesis that LLLT treatment could control myopia progression and comparing the abilities of OK lenses and LLLT to control the refractive error of myopia. Eighty-one children (81 eyes) who wore OK lenses, 74 children (74 eyes) who underwent LLLT treatment, and 74 children (74 eyes) who wore single-vision distance spectacles for 6 months were included. Changes in axial length (AL) were 0.23 ± 0.06 mm for children wearing spectacles, 0.06 ± 0.15 mm for children wearing OK lens, and − 0.06 ± 0.15 mm for children treated with LLLT for 6 months. Changes in subfoveal choroidal thickness (SFChT) observed at the 6-month examination were − 16.84 ± 7.85 μ m , 14.98 ± 22.50 μ m , and 35.30 ± 31.75 μ m for the control group, OK group, and LLLT group, respectively. Increases in AL at 1 month and 6 months were significantly associated with age at LLLT treatment. Changes in AL were significantly correlated with the baseline spherical equivalent refraction (SER) and baseline AL in the OK and LLLT groups. Increases in SFChT at 1 month and 6 months were positively associated with age at enrolment for children wearing OK lens. At 6 months, axial elongation had decelerated in OK lens-wearers and LLLT-treated children. Slightly better myopia control was observed with LLLT treatment than with overnight OK lens-wearing. Evaluations of age, SER, and AL can enhance screening for high-risk myopia, improve the myopia prognosis, and help determine suitable control methods yielding the most benefits.

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Section: Introductionmentioning

confidence: 99%

Orthokeratology and Low-Intensity Laser Therapy for Slowing the Progression of Myopia in Children

Xiong

Mao

Liao

et al. 2021

BioMed Research International

107

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“…The tables contain the number of metabolites involved in a given pathway and detected in serum or AH, as well as the results of pathway analysis (p-value and pathway impact value). Comparison of metabolic pathway analysis results for serum and AH samples identified 14 common pathways, mainly associated with decreased energy metabolism, increased oxidative stress, abnormal amino acid metabolism [37] or related to dopamine receptor D2 [35]. Metabolic pathways identified in the case of both serum and AH samples are highlighted in bold.…”

Section: Gc-tof-msmentioning

confidence: 99%

“…Dai et al [35] identified two metabolites, γ-glutamyltyrosine and 12-oxo-20-trihydroxy-leukotriene B4, as potential biomarkers of myopia. In turn, the metabolic alterations associated with high myopia included phospholipid, diacylglycerol, amino acid and vitamin metabolism.…”

Section: Metabolomicsmentioning

confidence: 99%

“…To the best of our knowledge, none of the previous studies used NMR to study this condition. The vast majority of researchers applied untargeted methods, such as CE-MS [33], LC-MS [33,35,36] or GC-MS [34,37,38], to analyze human serum [35][36][37][38] or AH [33,34]. We found only one published study using a targeted method to analyze human serum [39] and only one untargeted study in an animal model [40].…”

Section: Metabolomicsmentioning

confidence: 99%

“…For CE-MS analysis, the samples were mixed with the internal standard solution, and the material for LC-MS was only centrifuged, and then, the supernatant was analyzed directly [33]. The more complex preparation procedures described in the literature include deproteinization with methanol [35], methanol/chloroform or methanol/acetonitrile/water extraction buffer [36], followed by drying under nitrogen in most cases [35,36]. Then, the residues are dissolved and centrifuged, and the supernatants are used for further analyses [35].…”

Section: Metabolomicsmentioning

confidence: 99%

See 2 more Smart Citations

Omics in Myopia

Grochowski

Pietrowska

Kowalczyk

et al. 2020

JCM

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Myopia is a globally emerging issue, with multiple medical and socio-economic burdens and no well-established causal treatment thus far. A better insight into altered biochemical pathways and underlying pathogenesis might facilitate early diagnosis and treatment of myopia, ultimately leading to the development of more effective preventive and therapeutic measures. In this review, we summarize current data about the metabolomics and proteomics of myopia in humans and present various experimental approaches and animal models, along with their strengths and weaknesses. We also discuss the potential applicability of these findings to medical practice and suggest directions for future research.

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