Serum metabonomics analysis of quercetin against the toxicity induced by cadmium in rats

Abstract

This study aimed to investigate the protective effect of quercetin against the toxicity induced by chronic exposure to low levels of cadmium in rats by an ultra performance liquid chromatography mass spectrometer. Rats were randomly divided into six groups as follows: control group (C), low dose of quercetin group (Q1: 10 mg/kg·bw), high dose of quercetin group (Q2: 50 mg/kg·bw), cadmium chloride group (D), low dose of quercetin plus cadmium chloride group (DQ1), and high dose of quercetin plus cadmium chloride group (DQ2). Cadmium chloride (CdCl2) was administered to rats by drinking water ad libitum in a concentration of 40 mg/L. The final amount of CdCl2 ingested was estimated from the water consumption data to be 4.85, 4.91, and 4.89 mg/kg·bw/day, for D, DQ1, and DQ2 groups, respectively. After a 12‐week treatment, the serum samples of rats were collected for metabonomics analysis. Ten potential biomarkers were identified for which intensities were significantly increased or reduced as a result of the treatment. These metabolites included isorhamnetin 4′‐O‐glucuronide, 3‐indolepropionic acid, tetracosahexaenoic acid, lysophosphatidylcholine (LysoPC) (20:5), lysoPC (18:3), lysophosphatidylethanolamine (LysoPE) (20:5/0:0), bicyclo‐prostaglandin E2, sulpholithocholylglycine, lithocholyltaurine, and glycocholic acid. Results indicated that quercetin exerted a protective effect against cadmium‐induced toxicity by regulating lipid and amino acid metabolism, enhancing the antioxidant defense system and protecting liver and kidney function.

Protective Effects of Lactobacillus plantarum CCFM8610 against Acute Toxicity Caused by Different Food-Derived Forms of Cadmium in Mice

“…Kato et al treated rats with CdCl 2 at a low dose for 90 days and found that amino malonic acid was a biomarker for the toxicity of Cd alone [31]. Jia et al also found that quercetin could relieve Cd toxicity by regulating the metabolism of lipids and amino acids [32]. In this study, the effects of different foodborne forms of Cd treatment on serum metabolites were investigated.…”

Hawthorn flavonoid ameliorates cognitive deficit in mice with Alzheimer's disease by increasing the levels of Bifidobacteriales in gut microbiota and docosapentaenoic acid in serum metabolites

“…46 PC and phosphatidylethanolamine (PE) also play essential roles in the recognition and transduction of cell membrane signals, and they are hydrolyzed to generate LPC and LPE. 47 PC could protect neurons from the toxic effects of Aβ, 48 whereas LPC could inhibit LPS-mediated inflammatory responses and abnormal activation of microglia. 49 Therefore, HF improves the abnormal activation of microglia, reduces inflammation, and protects cell membranes by regulating phospholipid metabolism in AD mice.…”

Quercetin ameliorated cardiac injury via reducing inflammatory actions and the glycerophospholipid metabolism dysregulation in a diabetic cardiomyopathy mouse model

“…39 It was also reported that quercetin protected liver and kidney function from cadmium-induced toxicity by regulating lipid and amino acid metabolism, thus enhancing the antioxidant defense system. 40 In addition, quercetin was shown to alter the metabolites of human umbilical vein endothelial cells incubated with high glucose or TNF-α towards a less inflamed phenotype, both alone and in the presence of pro-inflammatory stimulation. 41 In the present study, we identified 23 potential differential metabolites in the serum of DCM mice.…”

“…39 It was also reported that quercetin protected liver and kidney function from cadmium-induced toxicity by regulating lipid and amino acid metabolism, thus enhancing the antioxidant defense system. 40 In addition, quercetin was shown to alter the metabolites of human umbilical vein endo-…”