Serum miR-21, miR-26a and miR-101 as potential biomarkers of hepatocellular carcinoma

Zhuang, Chunbo; Jiang, Weichao; Huang, Da; Xu, Luming; Yang, Qianqian; Zheng, Lei; Wang, Xiaobei; Hu, Lihua

doi:10.1016/j.clinre.2015.11.002

Cited by 43 publications

(30 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-26a regulated the proliferation and growth of cancer cells by targeting chromodomain helicase DNA binding protein 1 (35), growth regulation by estrogen in breast cancer 1 (35) and β-catenin (36). A number of studies demonstrated that the expression levels of miR-26a decreased in various types of tumor (23,37,38), whereas ANXA1 protein was overexpressed (39)(40)(41). Combined with the results of the present study suggesting that the miR-26a mimic may reduce the expression levels of ANXA1, a negative correlation was identified between miR-26a and ANXA1 in numerous tumors.…”

Section: Discussionsupporting

confidence: 78%

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

et al. 2018

View full text Add to dashboard Cite

Abstract. The aim of the present study was to investigate the expression and role of microRNA-26a (miR-26a) in lung cancer, and to verify whether differentially expressed in adenocarcinoma of the lung (DAL-1) is the target protein of miR-26a. mRNA expression levels of miR-26a and DAL-1 were detected using reverse transcription-quantitative polymerase chain reaction. Protein expression levels of DAL-1 and annexin A1 (ANXA1) were evaluated by western blot analysis. Cell Counting Kit-8, Transwell and wound scratch healing assays were used to characterize the function of miR-26a in lung cancer cells. The association of DAL-1 with miR-26a or ANXA1 was determined by dual-luciferase reporter or two-dimensional gel electrophoresis assays. miR-26a revealed decreased expression levels in lung cancer tissues compared with normal lung tissues, and decreased expression levels in lung cancer cells compared with 16HBE cells. Inhibition of miR-26a promoted lung cancer cell growth, migration and invasion. The DAL-1 protein exhibited downregulated expression levels in lung cancer tissues. DAL-1 was not the direct target gene of miR-26a. The two-dimensional gel electrophoresis assay confirmed that DAL-1 and ANXA1 were associated proteins. Expression levels of the ANXA1 protein were increased following DAL-1 gene silencing. The altered expression level of miR-26a affected the expression of ANXA1, and not of DAL-1. miR-26a demonstrated decreased expression levels in lung cancer cells, and it has an important effect on the biological function of lung cancer cells. However, DAL-1 was not a target gene of miR-26a. As a DAL-1 associated protein, ANXA1 was regulated by miR-26a. IntroductionLung cancer has a high incidence of tumor recurrence and metastasis, and is the most common cause of cancer-associated morality worldwide (1). The overall 5-year survival rate among patients with lung cancer is <15%, and a high rate of metastasis is the primary cause of lung cancer-associated mortality (2). A previous study confirmed that differentially expressed in adenocarcinoma of the lung (DAL-1), a protein that belongs to the membrane-associated cytoskeleton protein 4.1 family, is an efficient suppressor of epithelial-mesenchymal transition (EMT) in lung cancer (3). EMT is a pivotal event in lung cancer metastasis progression (4-6). However, the regulators of DAL-1 in EMT remain unknown.Recently, a novel batch of endogenous small non-coding regulatory RNAs (microRNA's; miRNA's) have received attention in the development of cancer, miRNA's bind complementary sequences in target mRNAs, resulting in selective degradation or selective inhibition of their translation (7). The present study investigated the possible miRNAs of DAL-1 using bioinformatic assays, which included miR-26b, miR-26a, miR-96 and miR-223. Among them, the regulation of DAL-1 by miR-223 has been demonstrated to serve a role in gastric cancer (8). The present study revealed that the gene silencing of DAL-1 induced annexin A1 (ANXA1) protein expression levels to increase in H460 cells...

show abstract

Section: Discussionsupporting

confidence: 78%

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Accumulating studies have indicated that dysregulation of circulating miRNAs could be a biomarker of tumorigenesis, development and invasion in various cancers including prostate cancer, gastric cancer, ovarian cancer, breast cancer and lung cancer . A diagnostic value for circulating miR‐101‐3p/5p in HCC has also been reported . Both of these studies validated that a lower miR‐101‐3p/5p level had diagnostic potential for HCC.…”

Section: Discussionmentioning

confidence: 83%

Diagnostic value of strand‐specific miRNA‐101‐3p and miRNA‐101‐5p for hepatocellular carcinoma and a bioinformatic analysis of their possible mechanism of action

Yang

Pang

et al. 2017

FEBS Open Bio

View full text Add to dashboard Cite

There is accumulating evidence that miRNA might serve as potential diagnostic and prognostic markers for various types of cancer. Hepatocellular carcinoma (HCC) is the most common type of malignant lesion but the significance of miRNAs in HCC remains largely unknown. The present study aimed to establish the diagnostic value of miR‐101‐3p/5p in HCC and then further investigate the prospective molecular mechanism via a bioinformatic analysis. First, the miR‐101 expression profiles and parallel clinical parameters from 362 HCC patients and 50 adjacent non‐HCC tissue samples were downloaded from The Cancer Genome Atlas (TCGA). Second, we aggregated all miR‐101‐3p/5p expression profiles collected from published literature and the Gene Expression Omnibus and TCGA databases. Subsequently, target genes of miR‐101‐3p and miR‐101‐5p were predicted by using the miRWalk database and then overlapped with the differentially expressed genes of HCC identified by natural language processing. Finally, bioinformatic analyses were conducted with the overlapping genes. The level of miR‐101 was significantly lower in HCC tissues compared with adjacent non‐HCC tissues (P < 0.001), and the area under the curve of the low miR‐101 level for HCC diagnosis was 0.925 (P < 0.001). The pooled summary receiver operator characteristic (SROC) of miR‐101‐3p was 0.86, and the combined SROC curve of miR‐101‐5p was 0.80. Bioinformatic analysis showed that the target genes of both miR‐101‐3p and miR‐101‐5p are involved in several pathways that are associated with HCC. The hub genes for miR‐101‐3p and miR‐101‐5p were also found. Our results suggested that both miR‐101‐3p and miR‐101‐5p might be potential diagnostic markers in HCC, and that they exert their functions via targeting various prospective genes in the same pathways.

show abstract

“…A previous study suggested low circulating levels of miR‐26a in a Korean population with HCC was associated with poor liver transplant‐free survival . Multiple Chinese studies have suggested that circulating levels of miR‐26a in combination with other miRNAs can be helpful in differentiating cases of chronic hepatitis B or C and HCC, even at early cancer stages . Further evaluation of circulating miR‐26a as a tool to predict HCC recurrence and survival may be warranted.…”

Section: Discussionmentioning

confidence: 99%

Lower expression of tumor microRNA‐26a is associated with higher recurrence in patients with hepatocellular carcinoma undergoing surgical treatment

Jones

Nabinger

Lee

et al. 2018

Journal of Surgical Oncology

View full text Add to dashboard Cite

show abstract

Serum miR-21, miR-26a and miR-101 as potential biomarkers of hepatocellular carcinoma

Cited by 43 publications

References 36 publications

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

Diagnostic value of strand‐specific miRNA‐101‐3p and miRNA‐101‐5p for hepatocellular carcinoma and a bioinformatic analysis of their possible mechanism of action

Lower expression of tumor microRNA‐26a is associated with higher recurrence in patients with hepatocellular carcinoma undergoing surgical treatment

Contact Info

Product

Resources

About