Serum Paraoxonase Activity and Oxidative DNA Damage in Patients With Laryngeal Squamous Cell Carcinoma

Karaman, Emin; Uzun, Hafize; Papila, İrfan; Balcı, Huriye; Özdilek, Alper; Genc, Habibe; Yanardağ, Halıl; Papila, Çiğdem

doi:10.1097/scs.0b013e3181f4040a

Cited by 24 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have reported associations between PON1 genetic polymorphisms or PON1 enzyme activity and oxidative stress, but the results are still inconclusive. Serum PON1 activity has been negatively correlated with urinary 8-OHdG levels in patients with Alzheimer’s disease [ 39 ] and laryngeal squamous cell carcinoma [ 40 ]. Bhattacharyya et al found that the PON1 192 RR genotype was associated with a lower level of oxidative stress [ 13 ], and Ji et al similarly reported that carriers of the PON1 rs662 Q allele have a significantly higher level of 8-OHdG in sperm DNA than R allele carriers [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Interactions between Paraoxonase 1 Genetic Polymorphisms and Smoking and Their Effects on Oxidative Stress and Lung Cancer Risk in a Korean Population

et al. 2015

View full text Add to dashboard Cite

BackgroundFew studies in epidemiology have evaluated the effects of gene-environment interaction on oxidative stress, even though this interaction is an important etiologic factor in lung carcinogenesis. We investigated the effects of the genetic polymorphisms of paraoxonase 1 (PON1), smoking, and the interaction between the two on lung cancer risk and oxidative stress.MethodsThis study’s subjects consisted of 416 newly diagnosed lung cancer patients and an equal number of matched controls. The GoldenGate assay was used for genotypic analyses of the PON1 gene. Urinary 8-hydroxydeoxyguanosine (8-OHdG) and thiobarbituric acid reactive substances levels were measured as indicators of oxidative stress.ResultsThe PON1 rs662 AA genotype showed a significantly lower risk of lung cancer than the GG genotype (OR = 0.60, 95% CI: 0.36–0.99). The protective effect of the PON1 rs662 AA genotype on lung cancer risk was limited to non-smokers. Lung cancer patients who had the rs662 A allele showed a dose-dependent association between smoking status and oxidative stress markers. Among non-smoking lung cancer patients, urinary 8-OHdG levels were significantly lower in individuals with the rs662 GA and AA genotypes than in those with the GG genotype. Furthermore, we found a significant interaction effect between PON1 rs662 and smoking status on urinary 8-OHdG levels in lung cancer patients.ConclusionsOur results suggest that the protective effect of PON1 rs662 SNP against lung carcinogenesis and the induction of oxidative stress might be modulated by the interaction between PON1 genetic polymorphisms and tobacco smoking.

show abstract

Section: Discussionmentioning

confidence: 99%

Interactions between Paraoxonase 1 Genetic Polymorphisms and Smoking and Their Effects on Oxidative Stress and Lung Cancer Risk in a Korean Population

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Many studies show that PON1 activity reduces the risk of cardiovascular disease caused by oxidative stress (14). Besides, PON1 activity reduces inflammatory processes, hyperthyroidism, certain neuropathies, diabetes, cancer, and many other diseases, by preventing oxidative stress (15)(16)(17)(18)(19)(20). For instance, Malik et al (4) report that the role of oxidative imbalance in the oral squamous cell carcinoma may be associated with the antioxidant status.…”

mentioning

confidence: 99%

Some Anticancer Agents Act on Human Serum Paraoxonase‐1 to Reduce Its Activity

Alım

Beydemır

2016

Chem Biol Drug Des

View full text Add to dashboard Cite

Human serum paraoxonase (hPON1) is an important antioxidant enzyme. It protects low-density lipoproteins against oxidative stress and prevents atherosclerosis development. Anticancer agents have cardiotoxic effects, and this situation can lead to significant complications. Our aim was to evaluate the in vitro effects of some of the anticancer agents such as cetuximab, paclitaxel, etoposide, docetaxel, and ifosfamide on the activity of hPON1 in this study. For this reason, PON1 was purified from human serum with a specific activity of 3654.2 EU/mg and 16.84% yield using simple chromatographic methods. The five chemotherapeutic agents dose dependently decreased in vitro hPON1 activity. IC50 values for cetuximab, paclitaxel, etoposide, docetaxel, and ifosfamide were 0.0111, 0.042, 0.226, 0.665, and 23.3 mm, respectively. Ki constants were 0.0194, 0.0165, 0.131, 0.291, and 8.973 mm, respectively. The inhibition mechanisms of cetuximab, etoposide, docetaxel, and ifosfamide were non-competitive, and for paclitaxel was competitive. Consequently, inhibition of hPON1 by these anticancer agents may explain some of the cardiotoxic actions of these drugs.

show abstract

“…16 Bansal et al reported that low PON1 activity may be considered as additional risk factor for development of vascular complications in type 2 diabetes mellitus. 17 Previous studies also suggested that low PON1 activity was associated with an increased risk for many cancer types, such as lung cancer, 21 laryngeal squamous cell carcinoma, 22 gastric 23 and pancreatic cancers. 24 Our results are supported by these previous studies.…”

Section: Resultsmentioning

confidence: 99%

“…18 In recent years, number of the studies investigating the relation between PON1 activity and cancer are increasing. Compared to the healthy controls, lower serum levels of PON1 were measured in patients suffering from breast 19,20 , lung 20,21 , laryngeal 22 , gastric 23 , pancreatic 24 and colorectal cancer. 20 Variations due to the presence of single nucleotide polymorphisms in antioxidant enzymes may contribute to the inter-individual differences between the transcript levels and enzyme activities, which might play a significant role in the development of cancer.…”

Section: Introductionmentioning

confidence: 99%

Association Between PON1 L55M Polymorphism and PON1 Enzyme Activity in Patients with Leukemia.

Eras¹,

Tombak²,

Yalın³

et al. 2017

UHOD

View full text Add to dashboard Cite

Paraoxonase 1 (PON1) is an important antioxidant enzyme which has a role in preventing the effects of systemic oxidative stress. The purpose of our study was to investigate the possible association between PON1 L55M polymorphism and leukemia development and to determine the relationship between PON1 genotypes and PON1 enzyme activities. Genotypes of 102 cases and 112 healthy controls were determined by PCR-RFLP. PON1 enzyme activity was determined according to Eckerson's method. The ratio of MM genotype belonging to PON1 L55M polymorphism in control group was 6.3% and was 7.8% in patients with leukemia (p= 0.39). PON1 enzyme activity was 118.8 ± 115.1 U/mL in control group, while decreased to 75.6 ± 64.4 U/mL in patients with leukemia (p= 0.004). PON1 enzyme activities of the individuals with MM genotypes belonging to PON1 L55M polymorphism was 57.43 ± 21.61 U/ mL in control group and decreased to 39.18 ± 45.61 U/mL in leukemic patients. Our results suggest that, PON1 L55M polymorphism genotype ratios do not affect leukemia development. However, reduced PON1 enzyme activity and also the combination of PON1 L55M polymorphism with reduced PON1 enzyme activity are associated with the increased risk of leukemia. Furthermore, older age may be a risk factor for developing leukemia. PON1 enzim aktivitesi Eckerson yöntemi ile ölçüldü. PON1 L55M polimorfizmine ait MM genotip oranları kontrol grubunda %6.3 iken lösemili hastalarda % 7.8 idi (p= 0.39). PON1 enzim aktivitesi kontrol grubunda 118.8 ± 115.1 U/mL iken lösemili hastalarda düşük olarak saptandı (75.6 ± 64.4 U/mL, p= 0.004). PON1 L55M polimorfizmine ait MM genotipli bireylerin PON1 enzim aktivitesi kontrol grubunda 57.43 ± 21.61 U/mL iken lösemili hastalarda düşük olarak saptandı (39.18 ± 45.61 U/mL). Bizim bulgularımız PON1 L55M polimorfizmi genotip oranlarının lösemi gelişimini etkilemediğini göstermektedir. Bununla birlikte, düşük PON1 enzim aktivitesi tek başına ve PON1 L55M polimorfizmi ile birlikte lösemi riski artışı ile ilişkilidir. Ayrıca ileri yaş lösemiye yakalanma olasılığını yükseltir.

show abstract

Serum Paraoxonase Activity and Oxidative DNA Damage in Patients With Laryngeal Squamous Cell Carcinoma

Abstract: We conclude that, in patients with laryngeal squamous cell carcinoma, the oxidant/antioxidant balance was impaired in favor of lipid peroxidation and DNA damage.

Cited by 24 publications

References 20 publications

Interactions between Paraoxonase 1 Genetic Polymorphisms and Smoking and Their Effects on Oxidative Stress and Lung Cancer Risk in a Korean Population

Interactions between Paraoxonase 1 Genetic Polymorphisms and Smoking and Their Effects on Oxidative Stress and Lung Cancer Risk in a Korean Population

Some Anticancer Agents Act on Human Serum Paraoxonase‐1 to Reduce Its Activity

Association Between PON1 L55M Polymorphism and PON1 Enzyme Activity in Patients with Leukemia.

Contact Info

Product

Resources

About