Serum profile of metabolites and hormones in obese (Iberian) and lean (Landrace) growing gilts fed balanced or lysine deficient diets

Fernández‐Fígares, I.; Lachica, M.; Nieto, R.; Rivera-Ferré, Marta G.; Aguilera, J. F.

doi:10.1016/j.livsci.2006.10.002

Cited by 74 publications

(76 citation statements)

References 38 publications

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“…GNG and glycogen degradation It was previously shown that differences related to genotype for glycemia and plasma insulin levels existed when Iberian and Landrace pigs were compared (Fernández-Fígares et al, 2007). In the present study, incubating hepatocytes with glucagon (28.7 nM) for 24 h virtually depleted glycogen stores (data not shown).…”

Section: Resultssupporting

confidence: 47%

“…Unfortunately, we did not measure glycogen synthetic capacity in our experiments. In vivo, however, Iberian had lower fasting serum glucose and greater insulin concentration than Landrace growing gilts (Fernández-Fígares et al, 2007). Similarly, serum glucose concentrations were generally lower in obese compared with lean pigs (Pond et al, 1980).…”

Section: Resultsmentioning

confidence: 86%

“…Iberian pigs have a high potential for fat accumulation and the ability to store excess fat during feed abundance enables the Iberian pigs survival during periods of scarcity. Low fasting glycemia of Iberian pigs compared with Landrace (Fernández-Fígares et al, 2007) could be related to an increased basal and insulinstimulated glucose transport early in the development of obesity, a common pattern in many animal models of obesity.…”

Section: Resultsmentioning

confidence: 99%

“…Before analysis of IGF-1, media was concentrated 12× by means of a rotary evaporator (SpeedVac SPD131DDA-230; Thermo, Milford, MA, USA). A previously validated (Fernández-Fígares et al, 2007) two-site immunoradiometric assay (cat. no.…”

Section: Urea Synthesismentioning

confidence: 99%

“…Nevertheless, there is a lack of information about differences in metabolic activity in hepatocytes from breeds with divergent metabolic profile. The Iberian pig is an obese slow growing Mediterranean breed with higher whole-body fat content than lean-type pigs and distinct hormone and metabolite profile (Fernández-Fígares et al, 2007). When comparing breeds with disparate metabolic profiles it is presumable that hepatocytes may behave differently.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic differences in hepatocytes of obese and lean pigs

González-Valero

Rodríguez-López

Lachica

et al. 2014

Animal

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There are important differences in terms of metabolic activity, energy utilization and capacity of protein and fat deposition when Iberian and modern pigs are compared. Primary culture of hepatocytes was used to evaluate hepatic function and sensitivity to hormones between breeds without the interference of circulating blood factors. Hepatocytes were isolated from pure Iberian (n = 10) and Landrace (n = 8) pigs of similar BW (24.5 ± 12.1 and 32.9 ± 6.1 kg BW, respectively), by collagenase perfusion. Monolayers were established in medium containing fetal bovine serum for 1 day and switched to serum-free medium for the remainder of the culture period. Hepatocytes were maintained in William's E supplemented with β-mercaptoethanol (0.1 mM), glutamine (2 mM), antibiotics (gentamicin, penicillin, streptomycin and amphotericin B), dimethyl sulfoxide (1 µg/ml), dexamethasone (10 −8 M), insulin (0.173 and 17.3 nM) and glucagon (0.287, 2.87 and 28.7 nM) for 24 to 48 h. Gluconeogenesis (GNG), glycogen degradation, triglycerides (TG) content and esterification, β-hydroxybutyrate (BHB) synthesis, IGF-1 synthesis, albumin and urea synthesis were determined. Iberian pigs had greater capacity of GNG than Landrace (24%, P < 0.05), although no difference in glycogen degradation was found (P > 0.10). TG content and esterification tended to be lower in hepatocytes from Iberian compared with Landrace pigs (12% and 31%, respectively; 0.10 < P < 0.05). Furthermore, addition of free fatty acids (CLA or linoleic acid, 0.2 mM) increased TG content (64%, P < 0.001) although no difference between fatty acids was found. When free fatty acids were compared, a trend toward increased esterification (41%, P = 0.078) was found for CLA. Although glucagon stimulated and insulin inhibited BHB synthesis, no difference between breeds was found (P > 0.10). IGF-1 synthesis was diminished in hepatocytes from Iberian compared with Landrace pigs (16%, P < 0.05). On the contrary, rate of albumin synthesis was greater in Iberian compared with Landrace pigs (58%, P < 0.05). Finally, the capacity of urea synthesis was lower in hepatocytes of Iberian compared with Landrace pigs (37%, P < 0.05). When ammonia was added to the media, urea concentration increased (648%, 1108% and 2791% when 0 mM was compared with 2.5, 5 and 10 mM, respectively). Urea synthesis increased on increasing ammonia content (55% and 325% when 0 mM was compared with 5 and 10 mM, respectively; P < 0.0001). In conclusion, the genetic background accounts for important differences in protein and energy metabolism pathways found in primary culture of hepatocytes from lean and obese pigs.Keywords: hepatocytes, metabolism, pig ImplicationsSeveral studies have shown the existence of numerous dissimilarities in metabolic activity between Iberian and modern pig breeds that might be explained by genotypic differences. Primary hepatocyte cultures are a valuable tool to study specific metabolic liver functions, without the interference of changing levels of metabolites and hormones found in vivo. These...

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Section: Resultssupporting

confidence: 47%

Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Urea Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Metabolic differences in hepatocytes of obese and lean pigs

González-Valero

Rodríguez-López

Lachica

et al. 2014

Animal

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Hypothalamic expression of porcine leptin receptor (LEPR), neuropeptide Y (NPY), and cocaine- and amphetamine-regulated transcript (CART) genes is influenced by LEPR genotype

Óvilo¹,

Fernández²,

Fernández³

et al. 2010

Mamm Genome

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The leptin receptor (LEPR) is a key gene in the control of food intake and energy homeostasis. The sequence variant LEPR{NM_001024587.1}:c.1987C>T has been associated with growth, fatness, and body composition in several pig populations. The purpose of this work was to confirm the phenotypic effects of this SNP in two new experimental backcrosses involving Iberian, Landrace, and Duroc breeds, and to evaluate the quantitative effects of the SNP on the hypothalamic expression of LEPR and two other downstream genes. Results indicate significant additive effects of the SNP on body weight, back fat thickness, and hypothalamic LEPR gene expression in both populations. Allele T fixed in the Iberian breed is systematically associated with a higher growth and fat deposition and leads to an intense reduction of LEPR hypothalamic expression, providing new functional evidence that supports the causality of the analyzed SNP with respect to previously reported and newly observed phenotypic effects. Also, some effects of the LEPR genotype on neuropeptide Y (NPY) and cocaine- and amphetamine-regulated transcript (CART) genes are detected, although they are conditioned by the breed. Finally, a change in mRNA structure and an increase in free energy is predicted for allele T, agreeing with a cis-acting functional effect on mRNA stability, which also supports the causality hypothesis. The lower expression of the LEPR gene in Iberian pigs fits with obesity by leptin resistance observed in this breed. A reduction in leptin signaling could thus be considered one of the determinants of the obese phenotype characteristic of Iberian breed.

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