Serum profile of pro- and anti-inflammatory cytokines in rats following implantation of low-temperature plasma-modified titanium plates

Walschus, Uwe; Hoene, Andreas; Patrzyk, Maciej; Finke, Birgit; Polák, Martin; Lucke, Silke; Nebe, Barbara; Schroeder, Karsten; Podbielski, Andreas; Wilhelm, L.; Schlosser, Michael

doi:10.1007/s10856-012-4600-z

Cited by 12 publications

(10 citation statements)

References 38 publications

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“…It is therefore conceivable that the increased adhesion due to hydrophilic surfaces elicits an increased inflammation. Additional data from a previous study supports a surface-dependent expression of cytokines in the serum of Lewis rats with different coated titanium implants [ 31 ]. While titanium discs coated with plasma polymerised allyl amine (positive charged, hydrophilic) induced a significant increase in pro-inflammatory cytokines IL-2 and IFN-γ, titanium discs coated with plasma polymerised acrylic acid (negative charged, hydrophobic) expressed steady and lower serum levels of IL-2 and a significant increase in anti-inflammatory IL-4 [ 31 ].…”

Section: Discussionsupporting

confidence: 60%

Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats

et al. 2021

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Orthopaedic implants and temporary osteosynthesis devices are commonly based on Titanium (Ti). For short-term devices, cell-material contact should be restricted for easy removal after bone healing. This could be achieved with anti-adhesive plasma-fluorocarbon-polymer (PFP) films created by low-temperature plasma processes. Two different PFP thin film deposition techniques, microwave (MW) and radiofrequency (RF) discharge plasma, were applied to receive smooth, hydrophobic surfaces with octafluoropropane (C3F8) or hexafluorohexane (C6F6) as precursors. This study aimed at examining the immunological local tissue reactions after simultaneous intramuscular implantation of four different Ti samples, designated as MW-C3F8, MW-C6F6, RF-C3F8 and Ti-controls, in rats. A differentiated morphometric evaluation of the inflammatory reaction was conducted by immunohistochemical staining of CD68+ macrophages, CD163+ macrophages, MHC class II-positive cells, T lymphocytes, CD25+ regulatory T lymphocytes, NK cells and nestin-positive cells in cryosections of surrounding peri-implant tissue. Tissue samples were obtained on days 7, 14 and 56 for investigating the acute and chronical inflammation (n = 8 rats/group). Implants with a radiofrequency discharge plasma (RF-C3F8) coating exhibited a favorable short- and long-term immune/inflammatory response comparable to Ti-controls. This was also demonstrated by the significant decrease in pro-inflammatory CD68+ macrophages, possibly downregulated by significantly increasing regulatory T lymphocytes.

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Section: Discussionsupporting

confidence: 60%

Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats

et al. 2021

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“…Furthermore, the serum profile of different pro- and anti-inflammatory cytokines after implantation of Ti plates with PPAAm films or plasma polymerized acrylic acid films (PPAAc) in rats was examined to characterize the systemic inflammatory response. It was found that the different plasma modifications caused distinct serum cytokine profiles, which were as a whole comparable between the PPAAm samples and the untreated controls, while the PPAAc samples differed markedly from both PPAAm and controls [32]. The serum cytokine profile for the PPAAm samples also indicated two distinct phases of the systemic inflammatory response, possibly caused by time-dependent changes in the physico-chemical properties of PPAAm as described earlier [30].…”

Section: Introductionmentioning

confidence: 67%

Plasma Polymerized Allylamine—The Unique Cell-Attractive Nanolayer for Dental Implant Materials

et al. 2019

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Biomaterials should be bioactive in stimulating the surrounding tissue to accelerate the ingrowth of permanent implants. Chemical and topographical features of the biomaterial surface affect cell physiology at the interface. A frequently asked question is whether the chemistry or the topography dominates the cell-material interaction. Recently, we demonstrated that a plasma-chemical modification using allylamine as a precursor was able to boost not only cell attachment and cell migration, but also intracellular signaling in vital cells. This microwave plasma process generated a homogenous nanolayer with randomly distributed, positively charged amino groups. In contrast, the surface of the human osteoblast is negatively charged at −15 mV due to its hyaluronan coat. As a consequence, we assumed that positive charges at the material surface—provoking electrostatic interaction forces—are attractive for the first cell encounter. This plasma-chemical nanocoating can be used for several biomaterials in orthopedic and dental implantology like titanium, titanium alloys, calcium phosphate scaffolds, and polylactide fiber meshes produced by electrospinning. In this regard, we wanted to ascertain whether plasma polymerized allylamine (PPAAm) is also suitable for increasing the attractiveness of a ceramic surface for dental implants using Yttria-stabilized tetragonal zirconia.

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“…Plasma spraying, which used to be one of most popular coating techniques for implants, can be considered as one type of plasma treatment, but it has been reported to have problems such as delamination [64]. Several other types of thin coating techniques using plasma to enhance osseointegration, have been introduced and have shown some promise such as plasma nitriding [15,65], titanium nitride oxide coatings [66], plasma polymerized hexamethyldisiloxane [67], plasma polymerized allylamine [68], and plasma polymerized acrylic acid [69]. Films formed by plasma polymerization are generally free of pinholes, have a strong degree of adherence to a wide variety of materials, and have a greater degree of resistance to chemical and physical treatment because of their cross-linked structure [70].…”

Section: Surface Treatmentmentioning

confidence: 99%

Plasma in dentistry

Cha

Park

2014

Clinical Plasma Medicine

129

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This review describes the contemporary aspects of plasma application in dentistry. Previous studies on plasma applications were classified into two categories, surface treatment and direct applications, and were reviewed, respectively according to the approach. The current review discussed modification of dental implant surface, enhancing of adhesive qualities, enhancing of polymerization, surface coating and plasma cleaning under the topics of surface treatment. Microbicidal activities, decontamination, root canal disinfection and tooth bleaching were reviewed as direct applications with other miscellaneous ones. Non-thermal atmospheric pressure plasma was of particular focus since it is gaining considerable attention due to the possibility for its use in living tissues. Future perspectives have also been discussed briefly. Although it is still not popular among dentists, plasma has shown promises in several areas of dentistry and is now opening a new era of plasma dentistry.

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Serum profile of pro- and anti-inflammatory cytokines in rats following implantation of low-temperature plasma-modified titanium plates

Cited by 12 publications

References 38 publications

Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats

Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats

Plasma Polymerized Allylamine—The Unique Cell-Attractive Nanolayer for Dental Implant Materials

Plasma in dentistry

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