Serum Protein Binding of Myoglobin, Hemoglobin and Hematin

Abstract

Using paper and starch-gel electrophoresis we have demonstrated binding of hemoglobin, myoglobin and hematin by human serum protein which is not a haptoglobin. This protein was present in all sera tested except that from two patients with hemolytic anemia and was also present in serum genetically deficient in haptoglobin. The binding capacity of this protein is low and contributes only slightly to the total hemoglobin binding capacity of normal serum. However, it may represent most or all of the binding in sera from patients with hemolytic disease.

Biochemical investigation of suspected rhabdomyolysis

“…20,21 Perturbations to glomerular ®ltration rate (GFR), renal tubular absorption and urine¯ow rate that may occur in rhabdomyolysis will alter this value, 22 as may the reported low-af®nity protein binding of myoglobin in plasma. 23,24…”

Spitting cobra (Naja nigricincta nigricincta) bites complicated by rhabdomyolysis, possible intravascular haemolysis, and coagulopathy

“…[22,25,2729] Proteases released from muscle injury cause hepatic dysfunction. [30] Hyperkalaemia, hypocalcaemia and hepatic inflammation are early complications, late complications being renal failure and diffuse intravascular coagulation probably caused by the activation of the clotting cascade by components released from the damaged muscles. [25,29] Our patients exhibited leukocytosis, very high CK values (peaking 2 3 days after the bite), raised serum myoglobin, low serum haptoglobin, raised AST, ALT and LDH, and an increased INR.…”

March Haemoglobinuria

“…Paper and starch gel electrophoresis were done as previously described (Wheby et al 1960). Paper strips were stained with benzidine or with bromphenol blue.…”