Serum protein layers on parylene-C and silicon oxide: Effect on cell adhesion

Delivopoulos, Evangelos; Ouberaï, Myriam; Coffey, Paul; Swann, Marcus J.; Shakesheff, Kevin M.; Welland, Mark E.

doi:10.1016/j.colsurfb.2014.12.020

Cited by 28 publications

(25 citation statements)

References 52 publications

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“…This work has been further extended by Hughes [41] who demonstrated how to modulate the pattern adhesion properties for HEK293 cells on parylene-C. Most recently, Delivopoulos demonstrated how the ratio of the fibronectin and albumin on the parylene-C and SiO 2 surfaces played an important role in cell patterning [42], Trantidou [43] demonstrated parylene-C could be laser modified for the micropatterning of rat ventricular fibroblasts and neonatal myocytes and Golda-Cepa [44] demonstrated how parylene-C when functionalized with oxygen plasma could promote cell growth for the MG-63 human osteosarcoma cell line.…”

Section: Cell Patterning and Biomaterials Parylene-cmentioning

confidence: 89%

Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches

et al. 2016

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Recent literature suggests that glia, and in particular astrocytes, should be studied as organised networks which communicate through gap junctions. Astrocytes, however, adhere to most surfaces and are highly mobile cells. In order to study, such organised networks effectively in vitro it is necessary to influence them to pattern to certain substrates whilst being repelled from others and to immobilise the astrocytes sufficiently such that they do not continue to migrate further whilst under study. In this article, we demonstrate for the first time how it is possible to facilitate the study of organised patterned human astrocytic networks using hNT astrocytes in a SiO2 trench grid network that is inlayed with the biocompatible material, parylene-C. We demonstrate how the immobilisation of astrocytes lies in the depth of the SiO2 trench, determining an optimum trench depth and that the optimum patterning of astrocytes is a consequence of the parylene-C inlay and the grid node spacing. We demonstrate high fidelity of the astrocytic networks and demonstrate that functionality of the hNT astrocytes through ATP evoked calcium signalling is also dependent on the grid node spacing. Finally, we demonstrate that the location of the nuclei on the grid nodes is also a function of the grid node spacing. The significance of this work, is to describe a suitable platform to facilitate the study of hNT astrocytes from the single cell level to the network level to improve knowledge and understanding of how communication links to spatial organisation at these higher order scales and trigger in vitro research further in this area with clinical applications in the area of epilepsy, stroke and focal cerebral ischemia.

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Section: Cell Patterning and Biomaterials Parylene-cmentioning

confidence: 89%

Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches

et al. 2016

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“…Several studies reported very effective adsorption of plasma proteins on Parylene-C surfaces [16,37,38]. Treating Parylene-C with an O 2 plasma, yields hydrophilic surfaces and has been demonstrated to decrease albumin adsorption [14].…”

Section: Resultsmentioning

confidence: 99%

Surface Nanostructuring of Parylene-C Coatings for Blood Contacting Implants

et al. 2018

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This paper investigates the effects on the blood compatibility of surface nanostructuring of Parylene-C coating. The proposed technique, based on the consecutive use of O2 and SF6 plasma, alters the surface roughness and enhances the intrinsic hydrophobicity of Parylene-C. The degree of hydrophobicity of the prepared surface can be precisely controlled by opportunely adjusting the plasma exposure times. Static contact angle measurements, performed on treated Parylene-C, showed a maximum contact angle of 158°. The nanostructured Parylene-C retained its hydrophobicity up to 45 days, when stored in a dry environment. Storing the samples in a body-mimicking solution caused the contact angle to progressively decrease. However, at the end of the measurement, the plasma treated surfaces still exhibited a higher hydrophobicity than the untreated counterparts. The proposed treatment improved the performance of the polymer as a water diffusion barrier in a body simulating environment. Modifying the nanotopography of the polymer influences the adsorption of different blood plasma proteins. The adsorption of albumin—a platelet adhesion inhibitor—and of fibrinogen—a platelet adhesion promoter—was studied by fluorescence microscopy. The adsorption capacity increased monotonically with increasing hydrophobicity for both studied proteins. The effect on albumin adsorption was considerably higher than on fibrinogen. Study of the proteins simultaneous adsorption showed that the albumin to fibrinogen adsorbed ratio increases with substrate hydrophobicity, suggesting lower thrombogenicity of the nanostructured surfaces. Animal experiments proved that the treated surfaces did not trigger any blood clot or thrombus formation when directly exposed to the arterial blood flow. The findings above, together with the exceptional mechanical and insulation properties of Parylene-C, support its use for packaging implants chronically exposed to the blood flow.

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“…McArdle等 [95] 利用DPI和电化学石英晶体微天平另外, Delivopoulos等 [94] 利用DPI技术研究了纤黏蛋白(Fn)和BSA在parylene-C/二氧化硅基底上的吸附, 为新型材料在生物工程研究中的应用提供指导. 马洪梅等 [91] 分别用含不同功能基团的硅烷对DPI芯片进行修饰, 研究了界面上配基种类对BSA吸附行为的影响.…”

Section: 的内在稳定性以及蛋白质与表面的相互作用有关unclassified