Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level

Abstract

Spot 14 (S14) is a protein involved in fatty acid synthesis and was shown to be induced by thyroid hormone in rat liver. However, the presence of S14 in human serum and its relations with thyroid function status have not been investigated.The objectives of this study were to compare serum S14 concentrations in patients with hyperthyroidism or euthyroidism and to evaluate the associations between serum S14 and free thyroxine (fT4) or thyroid-stimulating hormone (TSH) levels.We set up an immunoassay for human serum S14 concentrations and compared its levels between hyperthyroid and euthyroid subjects. Twenty-six hyperthyroid patients and 29 euthyroid individuals were recruited. Data of all patients were pooled for the analysis of the associations between the levels of S14 and fT4, TSH, or quartile of TSH.The hyperthyroid patients had significantly higher serum S14 levels than the euthyroid subjects (median [Q1, Q3]: 975 [669, 1612] ng/mL vs 436 [347, 638] ng/mL, P < 0.001). In univariate linear regression, the log-transformed S14 level (logS14) was positively associated with fT4 but negatively associated with creatinine (Cre), total cholesterol (T-C), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and TSH. The positive associations between logS14 and fT4 and the negative associations between logS14 and Cre, TG, T-C, or TSH remained significant after adjustment with sex and age. These associations were prominent in females but not in males. The logS14 levels were negatively associated with the TSH levels grouped by quartile (ß = −0.3020, P < 0.001). The association between logS14 and TSH quartile persisted after adjustment with sex and age (ß = −0.2828, P = 0.001). In stepwise multivariate regression analysis, only TSH grouped by quartile remained significantly associated with logS14 level.We developed an ELISA to measure serum S14 levels in human. Female patients with hyperthyroidism had higher serum S14 levels than the female subjects with euthyroidism. The serum logS14 concentrations were negatively associated with TSH levels. Changes of serum S14 level in the whole thyroid function spectrum deserve further investigation.

The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome

“…However, the expression level of S14R was demonstrated to be far lower than S14, approximately 1/10 in the liver and 1/100 in the adipose tissues at the mRNA level [37]. The use of our S14 immunoassay is reliable and has been reported to be related human thyroid function before [19]. Second, this is a cross-sectional study.…”

“…A competitive ELISA for human serum S14 was developed as previously described [19]. Briefly, 100 μl of human recombinant S14 proteins (100 μg/L, diluted in cold 1x PBS; cat no.…”

Common and differential transcriptional responses to different models of traumatic stress exposure in rats

“…It is possible that markers in the bloodstream directly related to changes in adrenal cell gene expression described here will ultimately prove useful for a diagnostic test. The products of several of the genes in the index, including Thrsp , Scd and Pah , can be measured in the serum or plasma 22 – 24 , which could make them, or similar molecules, a practical pathway for testing. Alternatively, metabolites in the pathways in which these stress-sensitive genes function are a potential test substrate.…”

Elevated serum S14 levels are associated with more severe liver steatosis by ultrasonography

“…We developed an ELISA to measure serum S14 levels in our lab as previously described 19 . In brief, polystyrene MaxiSorp 96-well plates (Nunc A/S, Roskilde, Denmark) were coated with 100 μL/well human recombinant S14 proteins (100 ng/mL, diluted in PBS; cat no.…”