Serum, tissue and body fluid concentrations of tigecycline after a single 100 mg dose

Rodvold, Keith A.; Gotfried, Mark H.; Cwik, Michael; Korth‐Bradley, Joan M.; Dukart, Gary; Ellis-Grosse, Evelyn J.

doi:10.1093/jac/dkl403

Cited by 273 publications

(202 citation statements)

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“…Resistance during treatment with daptomycin has raised similar concerns about its effectiveness for life-threatening MRSA infections (3). Low serum levels and the bacteriostatic activity of tigecycline against MRSA limit its use for the treatment of MRSA bacteremia (4).…”

mentioning

confidence: 99%

Synergy of β-Lactams with Vancomycin against Methicillin-Resistant Staphylococcus aureus: Correlation of Disk Diffusion and Checkerboard Methods

Huang

Chen

et al. 2016

J Clin Microbiol

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Modified disk diffusion (MDD) and checkerboard tests were employed to assess the synergy of combinations of vancomycin and ␤-lactam antibiotics for 59 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and Mu50 (ATCC 700699).Bacterial inocula equivalent to 0.5 and 2.0 McFarland standard were inoculated on agar plates containing 0, 0.5, 1, and 2 g/ml of vancomycin. Oxacillin-, cefazolin-, and cefoxitin-impregnated disks were applied to the surface, and the zones of inhibition were measured at 24 h. The CLSI-recommended checkerboard method was used as a reference to detect synergy. The MICs for vancomycin were determined using the Etest method, broth microdilution, and the Vitek 2 automated system. Synergy was observed with the checkerboard method in 51% to 60% of the isolates when vancomycin was combined with any ␤-lactam. The fractional inhibitory concentration indices were significantly lower in MRSA isolates with higher vancomycin MIC combinations (P < 0.05). The overall agreement between the MDD and checkerboard methods to detect synergy in MRSA isolates with bacterial inocula equivalent to McFarland standard 0.5 were 33.0% and 62.5% for oxacillin, 45.1% and 52.4% for cefazolin, and 43.1% and 52.4% for cefoxitin when combined with 0.5 and 2 g/ml of vancomycin, respectively. Based on our study, the simple MDD method is not recommended as a replacement for the checkerboard method to detect synergy. However, it may serve as an initial screening method for the detection of potential synergy when it is not feasible to perform other labor-intensive synergy tests.V ancomycin and other glycopeptide antibiotics are currently the standard treatment for methicillin-resistant Staphylococcus aureus (MRSA) (1). The emergence of MRSA with reduced vancomycin susceptibility (SA-RVS) raises concerns about the reliability of vancomycin for the treatment of MRSA (2). Resistance during treatment with daptomycin has raised similar concerns about its effectiveness for life-threatening MRSA infections (3). Low serum levels and the bacteriostatic activity of tigecycline against MRSA limit its use for the treatment of MRSA bacteremia (4).The combination of vancomycin and ␤-lactam antibiotics offers a potential option for management of MRSA infections (5). This concept is based on several in vitro and animal studies that demonstrated a synergistic effect of vancomycin-␤-lactam combinations against MRSA (6-11).The current study was designed to determine the utility of a modified disk diffusion (MDD) test to assess synergy and antagonism for combinations of vancomycin and ␤-lactam antibiotics among clinical isolates of MRSA (8, 12). The CLSI checkerboard method was used as the reference for the determination of synergy. We determined the vancomycin MIC breakpoint where synergy is most commonly observed in MRSA isolates. MATERIALS AND METHODSBacterial isolates. Fifty-nine unique clinical isolates of MRSA with vancomycin MICs of Ն0.5 g/ml were obtained from patients at the Kaohsiung Veterans General Hospital in Taiwan ...

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confidence: 99%

Synergy of β-Lactams with Vancomycin against Methicillin-Resistant Staphylococcus aureus: Correlation of Disk Diffusion and Checkerboard Methods

Huang

Chen

et al. 2016

J Clin Microbiol

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“…Tigecycline concentration in bone was also evaluated in an experimental rat model and a single-dose human study (7,8). The rat model showed an area under the curve in bone ≈250× higher than plasma (7).…”

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confidence: 99%

Multidrug-ResistantAcinetobacter baumanniiOsteomyelitis from Iraq

Schafer

Mangino

2008

Emerg. Infect. Dis.

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“…However, in contrast to LLE extraction, SPE method is time-and labour-consuming, because it includes numerous steps needed to perform the entire extraction process. The literature describes the procedure of sample preparation for LC-MS/MS determination of TIG, which involved the addition of acetonitrile (Conte et al 2005, Muralidharan et al 2005, Ozcimen et al 2014, an internal standard dissolved in acetonitrile (Rodvold et al 2006, Bhattacharya et al 2014 or trifluoroacetic acid (Hoffmann et al 2007, Xie et al 2014) to a sample. However, our test extraction of TIG from turkey plasma with all the above methods was ineffective, which manifested itself by additional, unidentified peaks appearing in chromatograms.…”

Section: Discussionmentioning

confidence: 99%

“…There are only a few reports on determination of TIG; the validated LC-MS/MS assay was developed for determination of TIG in human plasma (Conte et al 2005, Rodvold et al 2006, Hoffmann et al 2007, Pai 2014, Xie et al 2014, bone (Ji et al 2007, Ji et al 2008, Bhattacharya et al 2014) and skin blister (Sun et al 2005). Most of the methods for determination of TIG in a biological matrix mentioned above lack sufficient information about the settings of a detector, therefore any attempt to their adaptation to animal experiments may cause difficulties and often require optimization followed by repeated validation.…”

Section: Introductionmentioning

confidence: 99%

Determination of tigecycline in turkey plasma by LC-MS/MS: validation and application in a pharmacokinetic study

Jasiecka-Mikołajczyk

Jaroszewski

2017

Polish Journal of Veterinary Sciences

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Tigecycline (TIG), a novel glycylcycline antibiotic, plays an important role in the management of complicated skin and intra-abdominal infections. The available data lack any description of a method for determination of TIG in avian plasma. In our study, a selective, accurate and reversed-phase high performance liquid chromatography-tandem mass spectrometry method was developed for the determination of TIG in turkey plasma. Sample preparation was based on protein precipitation and liquid-liquid extraction using 1,2-dichloroethane. Chromatographic separation of TIG and minocycline (internal standard, IS) was achieved on an Atlantis T3 column (150 mm x 3.0 mm, 3.0 μm) using gradient elution. The selected reaction monitoring transitions were performed at 293.60 m/z → 257.10 m/z for TIG and 458.00 m/z → 441.20 m/z for IS. The developed method was validated in terms of specificity, selectivity, linearity, lowest limit of quantification, limit of detection, precision, accuracy, matrix effect, carry-over effect, extraction recovery and stability. All parameters of the method submitted to validation met the acceptance criteria. The assay was linear over the concentration range of 0.01-100 μg/ml. This validated method was successfully applied to a TIG pharmacokinetic study in turkey after intravenous and oral administration at a dose of 10 mg/kg at various time-points.

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Serum, tissue and body fluid concentrations of tigecycline after a single 100 mg dose

Cited by 273 publications

References 13 publications

Synergy of β-Lactams with Vancomycin against Methicillin-Resistant Staphylococcus aureus: Correlation of Disk Diffusion and Checkerboard Methods

Synergy of β-Lactams with Vancomycin against Methicillin-Resistant Staphylococcus aureus: Correlation of Disk Diffusion and Checkerboard Methods

Multidrug-ResistantAcinetobacter baumanniiOsteomyelitis from Iraq

Determination of tigecycline in turkey plasma by LC-MS/MS: validation and application in a pharmacokinetic study

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