Serum TNF-α, GTH and MDA of high-fat diet-induced obesity and obesity resistant rats

Wu, Peixian; Zhang, Fan; Dai, Yali; Han, Le; Chen, Shuo

doi:10.1016/j.jsps.2016.04.011

Cited by 23 publications

(22 citation statements)

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“…It was also observed that the hyperlipidemia regulated activity of SCU may be correlated with the hepatic blood flow and liver function. Nguyen [ 38 ] reported that hepatic fatty acids regulated overall lipid metabolism by binding nuclear receptors that modulate gene transcription, consistent with our results.…”

Section: Discussionsupporting

confidence: 92%

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats

Fan

Lin

et al. 2017

Med Sci Monit

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BackgroundNonalcoholic fatty liver disease (NAFLD) is a condition characterized by excessive fat accumulation in the form of triglycerides. The incidence of NAFLD and hyperlipidemia, with their associated risks of end-stage liver and cardiovascular diseases, is increasing rapidly. This study aimed to investigate the effects of scutellarin on the experimental NAFLD in high-fat diet fed and chronic stress rats, and its possible mechanism.Material/MethodsSprague-Dawley rats were fed with high-fat diet and subjected to chronic stress for 12 weeks, and administered orally with scutellarin for 4 weeks (n=8), and then blood and livers were harvested for analyzing. Enzyme activity assay, immunofluorescence, Western blot, and quantitative RT-PCR were performed to analyze the factors of the oxidant/antioxidant system and pathway.ResultsAfter the high-fat diet and chronic stress administration for 12 weeks, serum and liver lipid metabolism of treatment groups with the different doses of SCU effectively improved and the degree of oxidative damage reduced. Using Western blot assay and immunofluorescence (IF) staining assay, Nrf2, HO-1, and PI3K, and AKT proteins significantly increased after SCU treatment for 4 weeks (P<0.01). The hepatic mRNA expression of HO-1, NQO1, and Nrf2 in SCU treatment groups was upregulated significantly through quantitative RT-PCR assay (P<0.05). However, compared to the positive control group, no difference was detected in the SCU (100 or 300 mg/kg) groups (P>0.05). These results indicate that SCU protects against NAFLD in rats via attenuation of oxidative stress.ConclusionsThe antioxidant effects of SCU on NAFLD are possibly dependent on PI3K/AKT activation with subsequent Nrf2 nuclear translocation, which increases expression of HO-1 and NQO1. We therefore suggest that breviscapine may be a potentially useful therapeutic strategy for NAFLD and hyperlipidemia.

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Section: Discussionsupporting

confidence: 92%

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats

Fan

Lin

et al. 2017

Med Sci Monit

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“…Interestingly, no significant changes in MDA levels were observed in other studies. However, it is important to emphasize that the feeding time was longer (six months) and the composition of the diet was different in these studies, which indicates that the type of diet, as well as the feeding time, are important to induce changes in OS markers such as MDA levels [53].…”

Section: Discussionmentioning

confidence: 95%

Acute Administration of Tibolone Prevents Oxidative Stress in Ovariectomized Rats Fed High-Fat-and-Fructose Diet

Cruz

Pérez

Barrera

et al. 2018

Exp Clin Endocrinol Diabetes

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In addition to oxidative stress due to the increase of free radicals, estrogen deficiency is associated with changes in enzymatic activity, glutathione redox ratio (GSH/GSSG), and the content of oxidative markers such as malondialdehyde. Tibolone, a synthetic steroid, has been used as an elective treatment for the relief of menopausal symptoms. However, the acute effects of hormonal therapy with tibolone on metabolic parameters and oxidative stress markers associated with the first stages of estrogen deficiency are still unknown. The study aimed to evaluate if the acute administration of tibolone reduces oxidative stress in ovariectomized rats fed high-fat-and-fructose diet. Rats were fed a standard diet or a diet consisting of 10% lard-supplemented chow and 20% high-fructose syrup in the drinking water plus tibolone or vehicle for seven days. Weight, cholesterol, triglycerides, and glucose levels, as well as antioxidant enzymes and oxidative stress markers were quantified in the serum of each experimental group. It was observed that seven days of diet and tibolone treatment in the ovariectomized group reduced weight, triglycerides, cholesterol, glucose levels and advanced glycation end products but did not change GSH/GSSG ratio nor the enzymatic activity of superoxide dismutase. Also, both glutathione peroxidase and glutathione reductase activity decreased, as well as malondialdehyde levels. These results suggest that the acute treatment with tibolone prevented the changes in the metabolic parameters analyzed as well as the increase in the levels of malondialdehyde and AGEs induced by ovariectomy and high-fat diet.

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“…Numerous scientific studies demonstrate that the use of a high-fat diet (especially long-term) impairs antioxidant defence mechanisms and leads to oxidative stress in the body [ 7 , 8 ]. Excessive fat intake results in mitochondrial β-oxidation of free fatty acids and excessive electron flow involving cytochrome c oxidase.…”

Section: Discussionmentioning

confidence: 99%

“…There are also reports indicating that excessive fat consumption and obesity adversely affect the nervous system, increasing the risk of dementia and Alzheimer’s disease [ 5 , 6 ]. Impaired functioning of the body due to a high-fat diet is believed to result from the excessive production of reactive oxygen species (ROS) that accompany it [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium

Dworzański

Cholewińska

Fotschki

et al. 2020

Animals

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The aim of the study was to determine how feeding rats a high-fat diet supplemented with various forms of chromium affects DNA methylation and oxidation reactions as well as the histology of heart and brain tissue. The rats received standard diet or high-fat diet and chromium at 0.3 mg/kg body weight (BW) in form of chromium (III) picolinate, chromium (III)-methionine, or nano-sized chromium. The content of malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHDG), the level of global DNA methylation and the activity of selected DNA repair enzymes were determined in the blood. In the brain and heart, the content of MDA, PC, 8-OHDG, and levels of global DNA methylation were determined. The brain was subjected to histological examination. The use of a high-fat diet was found to intensify epigenetic changes and oxidation reactions in the heart and brain. It was concluded that epigenetic changes and oxidation of lipids, proteins, and DNA in the heart and brain of rats resulting from the use of a high-fat diet cannot be limited by supplementing the diet with chromium. It was established that the use of chromium to supplement a high-fat diet intensifies the negative epigenetic and oxidative changes in the heart and brain, especially in the case of chromium nanoparticles.

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Serum TNF-α, GTH and MDA of high-fat diet-induced obesity and obesity resistant rats

Cited by 23 publications

References 1 publication

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats

Acute Administration of Tibolone Prevents Oxidative Stress in Ovariectomized Rats Fed High-Fat-and-Fructose Diet

Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium

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