Sesamol-loaded solid lipid nanoparticles for treatment of skin cancer

Abstract

Role of reactive oxygen species (ROS) in skin carcinogenesis is well documented. Natural molecules, like sesamol, with marked antioxidant potential can be useful in combating skin cancers. In vitro antiproliferative (using MTT assay) and DNA fragmentation studies in HL 60 cell lines, confirmed the apoptotic nature of sesamol. However, it showed a significant flux across the mice skin upon topical application, such that its local availability in skin is limited. Former is attributed mainly to its properties like small size, low molecular weight (138.28), and a sufficient lipid and water solubility (log P 1.29; solubility 38.8 mg/ml). To achieve its maximum epicutaneous delivery, packaging it into a suitable carrier system is thus indicated. Sesamol-loaded solid lipid nanoparticles (S-SLN) were thus prepared with particle size of 127.9 nm (PI: 0.256) and entrapment efficiency of 88.21%. Topical application of S-SLN in a cream base indicated significant retention in the skin with minimal flux across skin as confirmed by the in-vivo skin retention and ex-vivo skin permeation studies. In vivo anticancer studies performed on TPA-induced and benzo(a)pyrene initiated tumour production (ROS mediated) in mouse epidermis showed the normalization (in histology studies) of skin cancers post their induction, upon treatment with S-SLN.

Dermal delivery of doxorubicin-loaded solid lipid nanoparticles for the treatment of skin cancer

“…Solid lipid nanoparticles as a new generation drug carrier can be good candidate for enhancement of bioavailability and permeability of Dox in skin cancer cells (Calder on-Col on et al, Geetha et al, 2015;Simões et al, 2015). In this study, Dox-SLN particles were successfully prepared by hot homogenisation method.…”

Advances in encapsulated dermal formulations in chemoprevention of melanoma: An overview

“…Geetha et al proved the need for sesamol‐loaded SLNs in skin cancer treatment since a significant flux across mouse skin upon topical application of sesamol was observed. The apoptotic nature of sesamol was confirmed by in vitro antiproliferative MTT assay and DNA fragmentation studies in HL‐60 cell lines …”

“…Geetha et al proved the need for sesamol-loaded SLNs in skin cancer treatment since a significant flux across mouse skin upon topical application of sesamol was observed. The apoptotic nature of sesamol was confirmed by in vitro antiproliferative MTT assay and DNA fragmentation studies in HL-60 cell lines 26. Bharadwaj et al27 studied paclitaxel SLNs prepared by highspeed homogenization and ultrasonication methods, exhibiting biphasic release pattern.…”

Application of quality-by-design approach to optimize diallyl disulfide-loaded solid lipid nanoparticles

“…Natural antioxidants, especially dietary flavonoids and polyphenolic compounds, have been well-documented for their prophylactic and therapeutic actions in chemical-induced carcinogenesis. They concentrate on various carcinogen bioactivating steps, which are essential for the covalent binding of the carcinogen to cellular DNA (Geetha et al 2014). Allium species include garlic, onion, leeks, chives, and scallions which have great potential in treatment/prevention of cancers and cardiovascular diseases.…”