activity at 1, 5 and 25% level. At all the three concentrations, the essential oil showed more significant activity than 1% permethrin based product. [7] Previous reports on this plant occurring in different regions yielded, furanoditerpenoids, [8] terpenoids, [9][10] steroids [11] and aromatic esters.[1] However, no information was available on the preparation of an appropriate selective extract or fraction of the plant and its efficacy directed towards promoting hair growth or retarding hair fall or isolation of hair growth active compounds based on bioassay. In continuation of our interest on the isolation of biologically active molecules from medicinal plants for personal care applications, [12][13][14][15][16][17][18][19][20][21] we have undertaken the chemical examination of the rhizomes of H. spicatum. The present study describes the isolation of two known compounds, pentadecane (1) and an aromatic ester, ethyl p-methoxycinnmate (2) and hair growth studies of crude hexane extract, fractions and active compound.
MATERIALS AND METHODS
GeneralMelting points reported are uncorrected. UV spectra were recorded on Shimadzu UV spectrophotometer. IR spectra were recorded on a Shimadzu IR prestige 21. GC spectra were recorded in Shimadzu GC-17A capillary GC. 1 H and 13 C NMR spectra were recorded on a Bruker AMX 400 in CDCl 3 with TMS an internal standard and the chemical shifts being represented in parts per million (ppm, d values). GC-MS mass spectrum on a Jeol SX 102/DA 6000 mass spectrometer. Column chromatography was performed on silica gel (100-200 mesh, Acme synthetic chemicals, Mumbai, India). Fractions and purity of the compounds were monitored by analytical thin layer chromatography (TLC) and the spots were visualized by exposure to iodine vapour or 5% sulphuric acid in methanol followed by heating the plate at
INTRODUCTIONHedychium spicatum (Zingiberaceae), also known as spiked Ginger Lily is employed in the preparation of Abir, a fragrant coloured powder used during the Holi festival. The rhizomes possess strong aromatic odour and bitter camphoraceous smell. The rhizomes of the plant have been used in the preparation of cosmetic powders used for promoting hair growth. The rhizomes are also considered to have insect-repelling properties and are used for preservating clothes. The rhizomes are stomachic, carminative, stimulant and tonic, and are used in dyspepsia in the form of powder or decoction.[1] The rhizomes are much used in veterinary medicine.[2] The prior literature on Hedychium spicatum reveals that the cosmetic composition containing this plant extract regulates the firmness, tone or structure of skin or regulate wrinkles.[3] The compositions containing extract of Hedychium spicatum are useful for treating Tinea infections by topical application.[4] The ethanolic extract of rhizomes of H. spicatum possessed anti-inflammatory and analgesic activity. The anti-inflammatory activity was found in the hexane fraction and the compound hedychienone was found responsible for such activity and the analge...