Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells

Hibasami, Hiroshige; Yamada, Yayoi; Moteki, Hiroyuki; Katsuzaki, Hirotaka; Imai, Kunio; Yoshioka, Kazumi; Komiya, Takashi

doi:10.3892/ijmm.12.2.147

Cited by 16 publications

(20 citation statements)

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“…While the mechanisms of the growth inhibitory effect of costunolide in some cancer cells have previously been demonstrated (6,19,25,27), our study is the first time to describe this in human lung squamous carcinoma cells. In conclusion, costunolide induced apoptosis in SK-MES-1 cells accompany with a marked loss of G1/S phase cells.…”

Section: Discussionmentioning

confidence: 84%

“…Furthermore, costunolide potentiates 1,25-(OH)2D3-induced differentiation in HL-60 promyelocytic leukemia cells by modulating NF-κB activation (16,17). Previous studies have demonstrated that costunolide has anti-tumor potential by inhibiting proliferation, inducing apoptosis and reducing invasion and metastasis in a number of tumor cells including intestinal neoplasia and melanoma, leukemia and hepatocellular carcinoma cells, and cervical, prostate, bladder, colon and breast cancer cells (6,(18)(19)(20)(21)(22)(23)(24)(25). The effects of costunolide on human lung squamous carcinoma cells are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Costunolide induces G1/S phase arrest and activates mitochondrial-mediated apoptotic pathways in SK-MES 1 human lung squamous carcinoma cells

Hua

Zhang

et al. 2016

Oncology Letters

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Abstract. Despite the availability of several therapeutic options, a safer and more effective modality strategy is required for the treatment of lung cancer. Costunolide, a sesquiterpene lactone which isolated from the Saussurea lappa, has potent anticancer properties. In the present study, the effects of costunolide on cell viability, the cell cycle and apoptosis in SK-MES-1 human lung squamous carcinoma cells were investigated. Costunolide induced morphological changes and inhibited growth of SK-MES-1 cells growth. Flow cytometric analysis data demonstrated that costunolide significantly induced apoptosis of SK-MES-1 cells and induced cell cycle arrest at G1/S phase in a dose-dependent manner. Through upregulation in the expression of p53 and Bax, and downregulation in the expression of Bcl-2 and activation of caspase-3, costunolide-induced apoptosis was confirmed by western blot analysis. In addition, the significant loss of mitochondrial membrane potential indicated that costunolide may induce apoptosis via the mitochondria-dependent pathway in SK-MES-1 cells. These results highlight the potential effects of costunolide as an anti-cancer agent in a human lung squamous carcinoma cell line.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Costunolide induces G1/S phase arrest and activates mitochondrial-mediated apoptotic pathways in SK-MES 1 human lung squamous carcinoma cells

Hua

Zhang

et al. 2016

Oncology Letters

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“…It has been reported that some kinds of sesquiterpene compounds induced apoptosis in cancer cells (Furuya et al, 1994;Woynarowski et al, 1997) and costunolide was also proven to have preventive effects on intestinal carcinogenesis, (Mori et al, 1994) suggesting an apoptosis-inducing activity of costunolide. Studies demonstrate that costunolide induces apoptosis in HL-60 human leukemia cells by the ROS-mediated mitochondrial permeability transition and resultant cytochrome c release , activates the cleavage of poly-(ADP-ribose) polymerase (Park et al, 2001a), by depleting intracellular thiols (Choi et al, 2002a) and inducing the chromatin condensation (Hibasami et al, 2003;Komiya et al, 2004). Costunolide -induced apoptotic mechanisms are that the receptormediated pathway precedes the mitochondriadependent pathway, caused by the inhibition of telomerase activity via suppression of hTERT in NALM-6 cells (Kanno et al, 2008), alters the balance of antiapoptotic Bcl-2 (Choi et al, 2002a;Rasul et al, 2011) and induces apoptosis by activation of c-Jun N-terminal kinase (JNK) in leukemic U937 cells (Choi and Lee, 2009) and inhibition of the prosurvival Akt and nuclear factor kappa B signaling pathway in human endometriotic epithelial cells (Kim et al, 2011a).…”

Section: Effects On Apoptosismentioning

confidence: 99%

“…Furthermore, costunolide potentiated 1,25-(OH)2D3-induced differentiation in HL-60 promyelocytic leukemia cells (Choi et al, 2002b;Kim et al, 2008;Kim et al, 2002), via the interference with NF-κB activation. Further studies demonstrate that costunolide has antitumor potential by inhibiting proliferation, inducing apoptosis and reducing invasion and metastasis of a wide variety of tumor cells, including breast cancer cells (Bocca et al, 2004;Choi et al, 2005;Choi et al, 2011), hepatocellular carcinoma cells (Chen et al, 1995;Liu et al, 2011a;Sun et al, 2003), prostate cancer cells (Hsu et al, 2011), leukemia cells (Choi et al, 2002a;Choi and Lee, 2009;Choi et al, 2002b;Hibasami et al, 2003;Kanno et al, 2008;Kim et al, 2008;Kim et al, 2002;Kim et al, 2011b;Komiya et al, 2004;Lee et al, 2001;Song et al, 2001;Srivastava et al, 2006), gastric cancer cells (Ko et al, 2005;Rasul et al, 2011), colon cancer cells (Kawamori et al, 1995;Mori et al, 1994), melanoma cells (Chen et al, 2007;Park et al, 2001b), cervical cancer cells (Sun et al, 2003), KB and P388 tumor cell (Mondranondra et al, 1990), and platinum-resistant human ovarian cancer cells . It was also reported that costunolide inhibited angiogenic response by blocking the angiogenic factor signaling pathway (Jeong et al, 2002) and microtubule-interactting activity of costunolide (Bocca et al, 2004).…”

Section: Biological Functionsmentioning

confidence: 99%

Costunolide: A novel anti-cancer sesquiterpene lactone

Rasul

Parveen

2012

Bangladesh J Pharmacol

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Costunolide: A novel anti Costunolide: A novel anti Costunolide: A novel anti---cancer cancer cancer sesquiterpene lactone sesquiterpene lactone sesquiterpene lactone BJP IntroductionCancer is the leading cause of death and represents one of the most threatening diseases worldwide. In the recent report, it has been estimated that 12.7 million new cancer cases and 7.6 million cancer deaths occur in 2008, with 56% of new cancer cases and 63% of the cancer deaths occurring in the less developed regions of the world (Ferlay et al., 2010). Throughout the history of civilization, the human have relied on natural products as a primary source of medicine. Herbal medicines have been proven to be an important source of novel agents with a pharmaceutical potential. Many anti-cancer drugs in current use are either natural products or are derived from natural products. Herbal medicines, such as paclitaxel, camptothecin, vinca alkaloids, and etoposide hold great potential as promising agents for the treatment of cancer (Cragg and Newman, 2005). Natural products have tradition-ally provided a rich source of drugs for many diseases, including cancer and plants are an important source of novel natural products (Amin et al., 2009). In 2008, of the 225 drugs being developed, 164 were of natural origin, with 108 being derived from plants, 25 from bacterial sources, 7 from fungal and 24 from animal sources. And, to throw some more numbers around, of the 108 plant-based drugs, 46 were in preclinical development, 14 in phase I, 41 in phase II, 5 in phase III and two had already reached pre-registration stage (Harvey, 2008). David Newman and Gordon Cragg (Newman and Cragg, 2007) found that of 155 FDA-approved small molecule anti-cancer drugs, 47% were either natural products or directly derived there from.C o s t u n o l i d e ( 6 E , 1 0 E , 1 1 a R ) -6 , 1 0 -d i m e t h y l -3 -methylidene-3a, 4,5,8,9,11a-hexahydrocyclodeca [b] furan-2-one) is a sesquiterpene lactone. It is a colorless crystalline powder with molecular formula of C15H20O2 and molecular weight of 232.318 g/mol (Figure 1). This review is aimed to summarize the recent researches on costunolide focusing on anti-cancer activity and to lay emphasis on molecular mechanisms which suggests a promising for such pursuits in oncology. Literature was searched through Pub Med, Scopus and Elsevier Science Direct Journal. Access to the Elsevier Science Direct Journal was made possible through library of AbstractCurrently an ample interest is found among oncologists to explore anti-cancer compounds from herbs. Sesquiterpene lactones have accredited significant attention in pharmacological research. Costunolide is a well-known sesquiterpene lactone present in plants used as popular herbal remedy. Several plantderived compounds are currently successfully employed in cancer treatment. Growing evidences demonstrated that costunolide possesses anti-cancer activities by inhibiting cell proliferation, tumor invasion, angiogenesis, metastasis and inducing apoptosis of a variety of tumor...

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“…It also had cytotoxic properties against MCF-7, NCI-H460, and SF-268 cancer cell lines in vitro (Chang et al, 2010), P-388, L-1210 leukemia and SNU-5 stomach cancer cells and against human A549, SK-OV-3, SK-MEL-2, and HCT15 tumor cells . Costunolide (1) and the guainolide zaluzanin D (147) displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells by induction of chromatin condensation in the HL-60 cells (Hibasami et al, 2003).…”

Section: Antitumor and Cytotoxicmentioning

confidence: 99%

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

et al. 2013

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Sesquiterpenes (costunolide and zaluzanin D) isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia HL-60 cells

Cited by 16 publications

References 0 publications

Costunolide induces G1/S phase arrest and activates mitochondrial-mediated apoptotic pathways in SK-MES 1 human lung squamous carcinoma cells

Costunolide induces G1/S phase arrest and activates mitochondrial-mediated apoptotic pathways in SK-MES 1 human lung squamous carcinoma cells

Costunolide: A novel anti-cancer sesquiterpene lactone

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

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