Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain

Becker, Nathalie; Seitanidou, Tania; Murphy, Paula; Mg, Mattei; Topilko, Piotr; Ma, Nieto; Wilkinson, David G.; Charnay, Patrick; Gilardi-Hebenstreit, Pascale

doi:10.1016/0925-4773(94)90091-4

Cited by 135 publications

(76 citation statements)

References 58 publications

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“…In situ hybridization analysis of ephrin-B3 expression during organogenesis reveals that by far the strongest expression occurs in the dorsal and ventral midlines of the neural tube. This expression positions ephrin-B3 temporally and spatially so that it could interact with EphB3, which also shows neural expression (Becker et al, 1994;Ciossek et al, 1996), and suggests possible functions for ephrin-B3 in patterning of the midline of the central nervous system.…”

Section: Introductionmentioning

confidence: 79%

“…At day 8.5 (4 ± 10 somites) of development, m-EphB3 is expressed in the ventral midbrain, ventro-posterior forebrain and in the third and ®fth rhombomeres (Becker et al, 1994). In a study of day 16.5 embryos, a very restricted expression pattern for m-EphB3 was observed in the central nervous system, with expression seen in the mantle layer of the medulla, while in the spinal cord expression is in the gray matter mantle layer of the dorsal and ventral horns (Ciossek et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the distinctive expression patterns of the receptors soon suggested interesting roles in cellcell signaling. Almost all the receptors are expressed prominently in the developing or adult nervous system, suggesting roles in neural development or function (Becker et al, 1994;Cheng and Flanagan, 1994a;Ciossek et al, 1995;Ganju et al, 1994;Henkemeyer et al, 1994;Lai and Lemke, 1991;Maisonpierre et al, 1993;Nieto et al, 1992;Pasquale et al, 1992;Ruiz and Robertson, 1994;Sajjadi and Pasquale, 1993;Soans et al, 1994;Tuzi and Gullick, 1994;Zhou et al, 1994). Also, many of the receptors are expressed in highly restricted patterns at the stage of gastrulation and organogenesis, suggesting functions in patterning the embryo.…”

Section: Introductionmentioning

confidence: 99%

“…Also, many of the receptors are expressed in highly restricted patterns at the stage of gastrulation and organogenesis, suggesting functions in patterning the embryo. For example, many of the receptors display characteristic rhombomere-speci®c patterns in the hindbrain (Becker et al, 1994;Cheng and Flanagan, 1994a;Ganju et al, 1994;Henkemeyer et al, 1994;Nieto et al, 1992;Ruiz and Robertson, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube

et al. 1998

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The ephrins are a family of ligands that bind to Eph family receptor tyrosine kinases, and have been implicated in axon guidance and other patterning processes during vertebrate development. We describe here the identi®cation and characterization of murine ephrin-B3. The cDNA encodes a 340 amino acid transmembrane molecule, most closely related to the two other known transmembrane ligands, ephrin-B1 and ephrin-B2. In addition to homology in their extracellular receptor binding domains, these transmembrane ligands share striking homology between their cytoplasmic domains, with 31 of the last 34 amino acids of ephrin-B3 being identical to ephrin-B2, suggesting functional interactions of the cytoplasmic tail. While most Eph family ligands are promiscuous in their interactions with Eph receptors, binding studies with the ®ve receptors known to bind other transmembrane ligands only revealed a high a nity interaction of ephrin-B3 with EphB3, with a dissociation constant of approximately 1 nM. In situ hybridization of mouse embryos showed ephrin-B3 is expressed prominently at the dorsal and ventral midline of the neural tube, particularly in the¯oor plate, a structure with key functions in patterning the nervous system. The isolation of this ligand may help to elucidate the molecular basis of patterning activities at the neural tube midline.

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Section: Introductionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube

et al. 1998

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“…Eck/eph related RTKs also take part in vertebrate hindbrain segmentation. Several RTKs of this family have restricted expression domains in di erent rhombomeres (Becker et al, 1994). Especially Sek (Gilardi-Hebenstreit et al, 1992) seems to be playing a role in segmental restriction of gene expression in the odd-numbered rhombomeres r3 and r5 (Xu et al, 1995) as well as in patterning of the zebra®sh forebrain (Xu et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Identification of Elf-1 and B61 as high affinity ligands for the receptor tyrosine kinase MDK1

Ciossek

Ullrich

1997

Oncogene

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Mouse Developmental Kinase 1 (MDK1) is a receptor tyrosine kinase of the eck/eph subfamily expressed in a variety of tissues during early mouse embryogenesis. To obtain further insight into the function of MDK1, we determined identity and localisation of its physiological ligand(s). Staining whole embryos with fusion proteins between the extracellular domain of MDK1 and human secreted alkaline phosphatase revealed areas of high receptor binding in the caudal mesencephalon, the frontal neocortex and the limb buds. This staining was sensitive to treatment with phosphatidylinositol-speci®c phospholipase C. Using Scatchard analysis, high a nity binding of Elf-1 (1.7610 710 M) and B61 (2.2610 710 M) towards MDK1 could be demonstrated. However, the transmembrane ligand Lerk2 displayed no measurable a nity for MDK1. Elf-1 and B61 bind to the three full-length MDK1 isoforms with similar dissociation constants. Slightly lower a nities were observed for the two truncated receptors MDK1-T1 and MDK1-T2. The activation of MDK1 with Elf-1 or B61 leads to the rapid autophosphorylation of MDK1 as well as tyrosine phosphorylation of an unknown 62 kDa phosphoprotein in Rat1 cells. These ®ndings implicate MDK1 in patterning processes during early mouse embryogenesis and suggest MDK1 involvement in early organogenesis and midbrain development.

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PMP-22 expression in the central nervous system of the embryonic mouse defines potential transverse segments and longitudinal columns

et al. 1997

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PMP-22, a major constituent of peripheral nervous system (PNS) myelin, is also present in the central nervous system (CNS), in motoneurons of the cranial nerve motor nuclei and spinal cord (Parmantier et al. [1995] Eur. J. Neurosci. 7:1080-1088). The expression of PMP-22 in the CNS during embryonic and early postnatal development was investigated and showed a biphasic spatio-temporal pattern. The expression of PMP-22 started at embryonic day (E)11.5, in restricted longitudinal and transverse domains, in the ventricular zone of the spinal cord, rhombencephalon, mesencephalon and prosencephalon. In the mid- and forebrain, the PMP-22 signal was detectable in a longitudinal domain that followed ventrally the basal/alar boundary but could no longer be detected dorsally at some distance from the roof plate. Along the caudo-rostral axis, the territory in which PMP-22 was detected spanned the mesencephalon and the prosencephalon, extending caudally from the limit between the isthmus and the mesencephalon, and rostrally to the boundary between prosomeres 4 and 5 (p4/p5). In agreement with the prosomeric model of forebrain organization proposed by Puelles and Rubenstein ([1993] TINS 16:472-479), differences in the level of PMP-22 expression in p2, p3, and p4 clearly defined the p2/p3 and p3/p4 neuromeric boundaries. By E17.5, PMP-22 was no longer detected in the ventricular zone, but at E18.5 it began to be expressed in motoneurons of cranial nerve motor nuclei and, after birth, following a rostro-caudal gradient, in the ventral spinal cord.

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Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain

Cited by 135 publications

References 58 publications

Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube

Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube

Identification of Elf-1 and B61 as high affinity ligands for the receptor tyrosine kinase MDK1

PMP-22 expression in the central nervous system of the embryonic mouse defines potential transverse segments and longitudinal columns

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