Severity of Fusarium ear rot and concentration of fumonisin in grain of Argentinian maize hybrids

Presello, Daniel A.; Iglesias, Juliana; Botta, Grisela; Eyherabide, Guillermo Hugo

doi:10.1016/j.cropro.2006.08.004

Cited by 37 publications

(27 citation statements)

References 18 publications

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“…Since resistant and susceptible pools consisted in composite samples of hybrids, screening in S 5 generation seems to be a valuable approach to develop parental inbreds of ear rot resistant hybrids. Selection for disease resistance to F. proliferatum caused indirect responses in disease resistance to the ear rots caused by F. verticillioides and F. graminearum indicating, consistently with previous reports (Presello et al 2007;Löffler et al 2010), that broad-based resistance to Fusarium exists. Thus, selection after inoculation of a single species should be effective to develop maize genotypes with improved resistance to prevalent ear rotting Fusarium pathogens.…”

Section: Discussionsupporting

confidence: 80%

Responses to selection of S5 inbreds for broad-based resistance to ear rots and grain mycotoxin contamination caused by Fusarium spp. in maize

Presello

Pereyra²,

Iglesias

et al. 2010

Euphytica

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Random S 5 inbreds derived from three F 2 maize (Zea mays L.) populations (L1934 9 LP918, LP915 9 LP2541 and L7310 9 L7266) were selected for ear rot resistance after inoculation with a lowfumonisin producing isolate belonging to F. proliferatum. The four less susceptible and the four most susceptible inbreds from each population were crossed and F 1 seeds were pooled. Resistant and susceptible pools from each population were evaluated for disease severity (percentage of the ear visibly diseased) after inoculation with the isolate used for selection, and high toxigenic isolates belonging to F. verticillioides and F. graminearum. Grain mycotoxin concentration was assessed by ELISA. Differences in disease resistance to each fungus were observed between resistant and susceptible pools in most populations and environments indicating that selection after inoculation with a single species might be effective to develop broad-based resistance to Fusarium. Resistant pools exhibited, after inoculation with F. verticillioides, low grain fumonisin concentrations in most populations and years. Positive genotypic correlations between disease severity and fumonisin concentration (0.89 \ r g \ 0.98, depending on fungal species and year) indicate that selection for disease severity accounted for most of the variability for field fumonisin accumulation. Selection seemed to be also effective to reduce grain deoxynivalenol and zearalenone concentrations after inoculation with F. graminearum. Ratios between grain deoxynivalenol concentration and disease severity were lower in L7310 9 L7266 than those observed in the other populations suggesting that mechanisms affecting mycotoxin accumulation might exist in this population and additional responses should be feasible if including deoxynivalenol concentration as another selection parameter.

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Section: Discussionsupporting

confidence: 80%

Responses to selection of S5 inbreds for broad-based resistance to ear rots and grain mycotoxin contamination caused by Fusarium spp. in maize

Presello

Pereyra²,

Iglesias

et al. 2010

Euphytica

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“…Inbred L4637 was classified as resistant and L4674 as susceptible according to their field behavior after a fungal inoculation treatment in a previous experiment [14], [18], [19]. F. verticillioides inoculation was carried out through the silk channel, considering that it is the principal entry route of this fungal pathogen.…”

Section: Resultsmentioning

confidence: 99%

“…Two maize inbreds that had exhibited moderate resistance (L4637) and susceptibility (L4674) to Fusarium ear rot [14] were evaluated after inoculation with conidial suspensions of F. verticillioides . Completely randomized block design experiments with three replicates were conducted in Pergamino, Province of Buenos Aires.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance

et al. 2013

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Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.

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“…Ear rot effects may be reduced by developing and using less susceptible hybrids. High genotypic correlations between ear rot symptoms and grain mycotoxin concentration were observed in several host ranges (Robertson-Hoyt et al 2006a;Presello et al 2007) and QTLs were mapped at the same chromosome regions for both traits (Robertson-Hoyt et al 2006b). These results indicate that disease severity accounts for most of grain mycotoxin concentration and selection for disease resistance would produce indirect responses for grain mycotoxin accumulation.…”

Section: Introductionmentioning

confidence: 97%

Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm

Giomi

Kreff²,

Iglesias

et al. 2016

Euphytica

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Fusarium verticillioides and F. graminearum cause ear rots in maize (Zea mays L.) that reduce yield and contaminate the grain with mycotoxins produced by the fungi. To map QTLs for resistance to these ear rots, a F 5 mapping population, consisting of 298 recombinant inbreds obtained by randomly selfing of the cross between LP4637 (moderately resistant) and L4674 (susceptible), was genotyped with 250 single nucleotide polymorphism markers and phenotyped 2 years for disease severity after silk inoculation with conidial suspensions of F. verticillioides and F. graminearum. Four QTLs were mapped in chromosomes 2, 3 and 5, bins 2.03, 3.05, 3.07 and 5.07, explaining ranges of 11.2-11.8, 3.4-5.1, 6.2-7.6 and 3.8-5.0 of phenotypic variances (%), respectively, depending on year and fungus. Additive effects of each QTL ranged from 5.0 to 11.9 % of ear area covered by mold and no epistatic interactions were observed. The four QTLs were effective for both Fusarium species and environments indicating that LP4637 is a source of broad resistance to Fusarium stable across environments. These results are consistent with previous research reporting QTLs for ear rot resistance in the same chromosome regions from sources of resistance growing in North America, Africa, Europe and China.

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Severity of Fusarium ear rot and concentration of fumonisin in grain of Argentinian maize hybrids

Cited by 37 publications

References 18 publications

Responses to selection of S5 inbreds for broad-based resistance to ear rots and grain mycotoxin contamination caused by Fusarium spp. in maize

Responses to selection of S5 inbreds for broad-based resistance to ear rots and grain mycotoxin contamination caused by Fusarium spp. in maize

Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance

Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm

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