Sevoflurane attenuates hepatic ischemia reperfusion injury by the miR-122/Nrf2 pathway

Zhang, Kai; Xu, Xia; Hu, Lihong

doi:10.21037/atm-22-115

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…IRI, the imbalance between oxidant–antioxidant processes, accompanied by excessive production of ROS, 29 can be expressed as a pathophysiological stress that develops with local and systemic inflammation caused by the inability to ensure and restore blood flow to the organ. 30 The increase in ROS and inflammatory cells in the ischemic tissue affects blood flow in the ischemic tissue, causing even more inflammation.…”

Section: Discussionmentioning

confidence: 99%

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

Polat,

Şengel,

Küçük

et al. 2024

Science Progress

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Background: Ischemia–reperfusion injury (IRI) poses a significant challenge for physicians, necessitating the management of cell damage and the preservation of organ functions. Various surgical procedures, such as vascular surgery on extremities, temporary cross-clamping of the abdominal aorta in aortic surgery, and the use of a tourniquet in extremity surgeries, may induce lower limb IRI. The susceptibility to IRI is heightened in individuals with diabetes. This study aimed to investigate the effects of fullerenol C60 and sevoflurane on mouse muscle tissue in a lower limb IRI model and to assess their potential in preventing complications arising from ischemia–reperfusion in mice with streptozocin-induced diabetes. Methods: A total of 36 adult Swiss albino mice were randomly divided into six groups, each consisting of six mice: control group (group C), diabetes group (group D), diabetes–ischemia/reperfusion group (group DIR), diabetes–ischemia/reperfusion–fullerenol C60 group (group DIR-FC60), diabetes–ischemia/reperfusion–sevoflurane group (group DIR-S), and diabetes–ischemia/reperfusion–sevoflurane–fullerenol C60 group (DIR-S-FC60). Streptozocin (55 mg/kg) was intraperitoneally administered to induce diabetes in the relevant groups, with mice displaying blood glucose levels of 250 mg/dL or higher at 72 h were considered diabetic. After 4 weeks, all groups underwent laparotomy under anesthesia. In DIR-FC60 and DIR-S-FC60 groups, fullerenol C60 (100 mg/kg) was intraperitoneally administrated 30 min before the ischemia period. Sevoflurane, delivered in 100% oxygen at a rate of 2.3% and 4 L/min, was administered during the ischemia period in DIR-S and DIR-S-FC60 groups. In the IR groups, a microvascular clamp was placed on the infrarenal abdominal aorta for 120 min during the ischemia period, followed by the removal of the clamp and a 120-min reperfusion period. At the end of the reperfusion, gastrocnemius muscle tissues were removed for histopathological and biochemical parameter examinations. Results: Histopathological examination revealed a significant reduction in the disorganization and degeneration of muscle cells in the DIR-S-FC60 group compared to the DIR group (p = 0.041). Inflammatory cell infiltration was notably lower in the DIR-S, DIR-FC60, and DIR-S-FC60 groups than in the DIR group (p = 0.031, p = 0.011, and p = 0.013, respectively). The total damage scores in the DIR-FC60 and DIR-S-FC60 groups were significantly lower than in the DIR group (p = 0.018 and p = 0.008, respectively). Furthermore, the levels of malondialdehyde (MDA) in the DIR-S, DIR-FC60, and DIR-S-FC60 groups were significantly lower than in the DIR group (p < 0.001, p < 0.001, and p < 0.001, respectively). Catalase (CAT) enzyme activity in the DIR-S, DIR-FC60, and DIR-S-FC60 groups was higher than in the DIR group (p = 0.001, p = 0.014, and p < 0.001, respectively). Superoxide dismutase (SOD) enzyme activity in the DIR-FC60 and DIR-S-FC60 groups was also higher than in the DIR group (p < 0.001 and p = 0.001, respectively). Conclusion: Our findings indicate that administering fullerenol C60 30 min prior to ischemia in diabetic mice, in combination with sevoflurane, led to a reduction in oxidative stress and the correction of IR-related damage in muscle tissue histopathology. We believe that the administration of fullerenol C60 before IR, coupled with sevoflurane administration during IR, exerts a protective effect in mice.

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Section: Discussionmentioning

confidence: 99%

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

Polat,

Şengel,

Küçük

et al. 2024

Science Progress

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“…The authors stated that they achieved this by increasing the expression of HO –1 , supporting the transport of Nrf2 from the cytoplasm to the nucleus, and decreasing the expression of miR-122 and p53. 28 Wang et al reported that sevoflurane preconditioning reduced IR-induced hepatocyte apoptosis, hepatic inflammation, and the serum levels of GST, ALT, and AST. They stated that this might be related to the inhibition of the IR-mediated down-regulation of miR-124-3p.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Sevoflurane and Fullerenol C 60 on the Liver and Kidney in Lower Extremity Ischemia-Reperfusion Injury in Mice with Streptozocin-Induced Diabetes

Şengel,

Küçük,

Özdemir

et al. 2023

IJN

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Objective This study aimed to demonstrate whether fullerenol C60, sevoflurane anesthesia, or a combination of both had protective effects on the liver and kidneys in lower extremity ischemia-reperfusion injury (IRI) in mice with streptozocin-induced diabetes. Methods A total of 46 Swiss albino mice were divided into six groups as follows: control group (group C, n=7), diabetes group (group D, n=7), diabetes-ischemia/reperfusion (group DIR, n=8), diabetes-ischemia/reperfusion-fullerenol C60 (group DIR-FC60, n=8), diabetes-ischemia/reperfusion-sevoflurane (group DIR-S, n=8), and the diabetes-ischemia/reperfusion-fullerenol C60-sevoflurane (group DIR-S-FC60, n=8). Fullerenol C60 (100mg/kg) was administered intraperitoneally 30 min before the ischemia-reperfusion procedure to the fullerenol groups (DIR-FC60 and DIR-S-FC60). In the DIR groups, 2 hours (h) ischemia-2h reperfusion periods were performed. In the sevoflurane groups, sevoflurane was applied during the ischemia-reperfusion period with 100% O 2 . Liver and kidney tissues were removed at the end of the reperfusion procedure for biochemical and histopathological examinations. Results In liver tissue, hydropic degeneration, sinusoidal dilatation, pycnotic nuclei, prenecrotic cells, and mononuclear cell infiltration in parenchyma were significantly more frequent in group DIR than in groups D and group C. In terms of the histopathologic criteria examined, more positive results were seen in group DIR-FC60, and when group DIR-FC60 was compared with group DIR, the difference was significant. The best results in AST, ALT, glucose, TBARS levels, and SOD enzyme activities in liver tissue were in group DIR-FC60 compared with group DIR, followed by groups DIR-S-FC60 and DIR-S, respectively. Regarding TBARS levels and SOD enzyme activities in kidney tissue, the best results were in groups DIR-FC60, DIR-S-FC60, and DIR-S, respectively. Conclusion According to our findings, it is clear that fullerenol C60 administered intraperitoneally 30 min before ischemia, alone or together with sevoflurane, reduces oxidative stress in distant organ damage caused by lower extremity IRI, and reduces liver and kidney tissue damage in histopathologic examinations.

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“…The prepared preparations were examined under a research microscope (Olympus CX31-Japan) and their photographs were taken at 40 X magni cation. Then, the severity of the damage was evaluated according to the Suzuki Score (Zhang et al 2022).…”

Section: Methodsmentioning

confidence: 99%

Effect of Thymoquinon on TRPM Channels in Rats with Liver Ischemia

Caglar

Tümer

Tutuk

et al. 2023

Preprint

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Ischemia/Reperfusion (I/R) injury is surgery and clinically important problem. It was showed that Ca+ 2 concentration increases into cell with I/R injury. TRPM channels have a role in determining the amount of Ca+ 2 concentration into cell. Thymoquinone (Tmq) which was isolated from Nigella Sativa, particularly has the beneficial effects such as antioxidant, anticancerogenic, antiinflamatuar. We aimed to investigate the antioxidant and histopathologic effect of thymoquinone in hepatic I/R rat model. In addition the other purpose of the our study was determination effects of thymoquinone on levels of TRPM gene expression. Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Shame; Group 3: Hepatic I/R (45min/45min); Group 4: Tmq (50 mg/kg); Group 5: Tmq + I/R (ten days before from I/R at dose 50 mg/kg of Tmq by oral gavage.) Hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular a clamp in ischemia groups. The liver tissues and blood samples which were taken at the end of study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL and apoptosis parameters statistically decreased in Tmq and Tmq + I/R groups in comparision with I/R group in histopathological evaluation. It was obsorved that biochemical parameters, AST, ALT, GGT, LDH, creatinine and urea levels significantly increased in I/R group as compared with, shame, Tmq and Tmq + I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq + I/R groups as compared to I/R group. Based on our findings it was observed that application of Tmq in the treatment of liver diseases associated with I/R injury is important in terms of both ischemia and apoptosis and also using in the treatment of liver-related diseases. Additionally we showed that thymoquinone may inhibit Ca+ 2 entry into the cell by reducing TRPM2,6,7,8 gene expression. In conclusion this situation is brought to mind that cell injury related with I/R is reduced by Tmq.

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Sevoflurane attenuates hepatic ischemia reperfusion injury by the miR-122/Nrf2 pathway

Cited by 8 publications

References 32 publications

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

The Effect of Sevoflurane and Fullerenol C 60 on the Liver and Kidney in Lower Extremity Ischemia-Reperfusion Injury in Mice with Streptozocin-Induced Diabetes

Effect of Thymoquinon on TRPM Channels in Rats with Liver Ischemia

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