Sex determination in the silkworm, Bombyx mori: A female determinant on the W chromosome and the sex-determining gene cascade

Fujii, Tsuguru; Shimada, Toru

doi:10.1016/j.semcdb.2007.02.008

Cited by 67 publications

(63 citation statements)

References 53 publications

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“…A translocation event between the W and Z chromosomes was documented to result in a hybrid WZ chromosome causing a lethal phenotype when transmitted to female progeny . Additional rearrangements have been documented to cause male muscle dystrophy and abnormal oogenesis in B. mori females (Fujii and Shimada 2007).…”

Section: Discussionmentioning

confidence: 99%

A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis

et al. 2011

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Males are homogametic (ZZ) and females are heterogametic (WZ) with respect to the sex chromosomes in many species of butterflies and moths (insect order Lepidoptera). Genes on the Z chromosome influence traits involved in larval development, environmental adaptation, and reproductive isolation. To facilitate the investigation of these traits across Lepidoptera, we developed 43 degenerate primer pairs to PCR amplify orthologs of 43 Bombyx mori Z chromosome-linked genes. Of the 34 orthologs that amplified by PCR in Ostrinia nubilalis, 6 co-segregated with the Z chromosome anchor markers kettin (ket) and lactate dehydrogenase (ldh), and produced a consensus genetic linkage map of ~89 cM in combination with 5 AFLP markers. The O. nubilalis and B. mori Z chromosomes are comparatively co-linear, although potential gene inversions alter terminal gene orders and a translocation event disrupted synteny at one chromosome end. Compared to B. mori orthologs, O. nubilalis Z chromosome-linked genes showed conservation of tissuespecific and growth-stage-specific expression, although some genes exhibited species-specific expression across developmental stages or tissues. The O. nubilalis Z chromosome linkage map provides new tools for isolating quantitative trait loci (QTL) involved in sex-linked traits that drive speciation and it exposes genome rearrangements as a possible mechanism for differential gene regulation in Lepidoptera. Abstract Males are homogametic (ZZ) and females are heterogametic (WZ) with respect to the sex chromosomes in many species of butterflies and moths (insect order Lepidoptera). Genes on the Z chromosome influence traits involved in larval development, environmental adaptation, and reproductive isolation. To facilitate the investigation of these traits across Lepidoptera, we developed 43 degenerate primer pairs to PCR amplify orthologs of 43 Bombyx mori Z chromosomelinked genes. Of the 34 orthologs that amplified by PCR in Ostrinia nubilalis, 6 co-segregated with the Z chromosome anchor markers kettin (ket) and lactate dehydrogenase (ldh), and produced a consensus genetic linkage map of *89 cM in combination with 5 AFLP markers. The O. nubilalis and B. mori Z chromosomes are comparatively co-linear, although potential gene inversions alter terminal gene orders and a translocation event disrupted synteny at one chromosome end. Compared to B. mori orthologs, O. nubilalis Z chromosomelinked genes showed conservation of tissue-specific and growth-stage-specific expression, although some genes exhibited species-specific expression across developmental stages or tissues. The O. nubilalis Z chromosome linkage map provides new tools for isolating quantitative trait loci (QTL) involved in sex-linked traits that drive speciation and it exposes genome rearrangements as a possible mechanism for differential gene regulation in Lepidoptera.

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Section: Discussionmentioning

confidence: 99%

A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis

et al. 2011

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“…A comparison of sex-determining cascades in different insect groups reveals that diversity essentially starts at the level of regulation of tra splicing and that tra acts as receptor for various primary signals. These signals are very diverse and include X-chromosome dose [10 ], a male determining factor on the Y chromosome and/or autosome [36 ,57], and a feminizing factor on the W chromosome [47] in diploids, as well as complementary sex determination and genomic imprinting sex determination in haplodiploids [42 ,58,59 ]. Therefore, the conserved part of the insect sex-determining cascade must be extended to include tra, and from an evolutionary perspective, tra appears to serve as the gene around which flexibility in sex determination is manifested.…”

Section: Doublesexmentioning

confidence: 99%

Insect sex determination: it all evolves around transformer

Verhulst

Zande

Beukeboom

2010

Current Opinion in Genetics & Development

233

225

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“…Sex determination cascades that drive the somatic sexual differentiation are some of the most rapidly evolved developmental events (Revised in Fujii and Shimada 2007;Traut et al 2007). Nevertheless, recent studies indicate that some of the downstream sex determination genes are functionally similar in diverse species.…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

Cavaliere

Cara²,

Polito³

et al. 2009

Int. J. Dev. Biol.

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The intersex (ix) gene works in concert with doublesex (dsx) at the bottom of the sexdetermination hierarchy to control somatic sexual differentiation in Drosophila melanogaster females. Here we report the isolation and characterization of the Drosophila intersex (ix) homologue in the pest lepidopteron Maruca vitrata (Mvix). The Mvix gene exhibits major complexity with respect to the Drosophila homolog. It is expressed in males and females and its pre-mRNA is subject to differential splicing events which affect both the protein coding and the non-coding regions. Moreover, Northern blot experiments revealed the presence of a femalespecific transcript in pupae RNA, which appears to be the first described sex specific transcript of ix homologs characterized to date. The expression of Mvix cDNA in D.melanogaster transgenic flies indicates that the MvIX product, which shares a relatively high degree of homology with the D.melanogaster IX protein, is able to partially rescues the Drosophila mutant phenotype.

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Sex determination in the silkworm, Bombyx mori: A female determinant on the W chromosome and the sex-determining gene cascade

Cited by 67 publications

References 53 publications

A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis

A rearrangement of the Z chromosome topology influences the sex-linked gene display in the European corn borer, Ostrinia nubilalis

Insect sex determination: it all evolves around transformer

Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

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