Sex differences in the effect of ethanol injection and consumption on brain allopregnanolone levels in C57BL/6 mice

Finn, Deborah A.; Sinnott, Rachna S.; Ford, Matthew M.; Long, Season L.; Tanchuck, Michelle A.; Phillips, Tamara J.

doi:10.1016/j.neuroscience.2003.11.017

Cited by 85 publications

(102 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When taken in conjunction with the recent observations that ethanol consumption increased endogenous ALLO concentrations in B6 mice [10] and human adolescents [43,44], and that the inhibition of ALLO biosynthesis attenuated ethanol intake [13], the current findings are consistent with the hypothesis that alterations in endogenous ALLO levels may influence the regulatory processes governing ethanol consumption and reinforcement. The development of pharmacological interventions that manipulate endogenous ALLO concentrations may prove to be a beneficial treatment strategy.…”

Section: Discussionsupporting

confidence: 87%

Allopregnanolone influences the consummatory processes that govern ethanol drinking in C57BL/6J mice

Ford

Mark

Nickel

et al. 2007

Behavioural Brain Research

View full text Add to dashboard Cite

Although systemic allopregnanolone (ALLO; a positive modulator of GABA A receptors) has been shown to enhance ethanol-reinforced responding and to modulate drinking patterns in rodents, the effects of centrally administered ALLO on ethanol intake are not known. The current work examined the effects of intracranial ALLO on operant ethanol self-administration in food-and water-satiated mice, with a procedure designed to estimate ALLO's influence on appetitive versus consummatory processes. Male C57BL/6J (B6) mice were trained to press an ethanol-appropriate lever by being reinforced with 30-min of continuous access to a 10% ethanol solution. Following surgical implantation of a guide cannula aimed at the lateral ventricle and subsequent habituation to vehicle infusions, ALLO (50-400 ng; ICV) was delivered immediately prior to session start. ALLO doses of 100 and 400 ng were further evaluated for their effects on locomotor behavior within activity chambers. ALLO selectively modulated ethanol intake patterns associated with the onset and maintenance of self-administration, while leaving appetitive (i.e., ethanol seeking) measures unaltered. The effects of ALLO on drinking patterns were dissociable from changes in locomotor behavior, as evidenced by the absence of ALLO's influence on response frequency and horizontal distance traveled. These findings support the premise that manipulations in brain ALLO levels may influence the regulatory processes governing ethanol consumption.

show abstract

Section: Discussionsupporting

confidence: 87%

Allopregnanolone influences the consummatory processes that govern ethanol drinking in C57BL/6J mice

Ford

Mark

Nickel

et al. 2007

Behavioural Brain Research

View full text Add to dashboard Cite

show abstract

“…Female B6 mice had significantly greater intake of 6E, with a trend for an increase in 10E, when compared to their male counterparts. Nonetheless, these findings are consistent with clinical and preclinical studies documenting the existence of sex differences in sensitivity to a number of alcohol-related behaviors associated with neuroadaptation and reinforcement (e.g., Devaud et al, 2003;Finn et al, 2004aFinn et al, , 2004bGreen et al, 1999;Hashimoto and Wiren, 2007;Lancaster, 1995;Middaugh and Kelly, 1999;Rhodes et al, 2007;Vivian et al, 2001;Wiren et al 2006). …”

Section: Discussionsupporting

confidence: 82%

Voluntary ethanol consumption in 22 inbred mouse strains

Yoneyama

Crabbe

Ford

et al. 2008

Alcohol

276

269

View full text Add to dashboard Cite

Inbred strains are genetically stable across time and laboratories, allowing scientists to accumulate a record of phenotypes, including physiological characteristics and behaviors. To date, the C57/C58 family of inbred mouse strains has been identified as having the highest innate ethanol consumption, but some lineages have rarely or never been surveyed. Thus, the purpose of the present experiment was to measure ethanol preference and intake in 22 inbred mouse strains, some of which have never been tested for ethanol consumption. Male and female mice (A/J, BALB/cByJ, BTBR+T tf/tf , BUB/ BnJ, C57BL/6J, C57BLKS/J, C58/J, CZECH/Ei, DBA/2J, FVB/NJ, I/LnJ, LP/J, MA/MyJ, NOD/ LtJ, NON/LtJ, NZB/B1NJ, NZW/LacJ, PERA/Ei, RIIIS/J, SEA/GnJ, SM/J, and 129S1/SvlmJ) were individually housed and given unlimited access in a two-bottle choice procedure to one bottle containing tap water and a second containing increasing concentrations of ethanol (3%, 6%, 10%), 0.2% saccharin, and then increasing concentrations of ethanol (3%, 6%, 10%) plus 0.2% saccharin. Mice were given access to each novel solution for a total of 4 days, with a bottle side change every other day. Consistent with previous studies, C57BL/6J (B6) mice consumed an ethanol dose of > 10g/kg/day while DBA/2J (D2) mice consumed < 2g/kg/day. No strain voluntarily consumed greater doses of ethanol than B6 mice. While the C58 and C57BLKS strains showed high ethanol consumption levels that were comparable to B6 mice, the BUB and BTBR strains exhibited low ethanol intakes similar to D2 mice. The addition of 0.2% saccharin to the ethanol solutions significantly increased ethanol intake by most strains and altered the strain distribution pattern. Strong positive correlations (rs ≥ 0.83) were determined between consumption of the unsweetened versus sweetened ethanol solutions. Consumption of saccharin alone was significantly positively correlated with the sweetened ethanol solutions (rs = 0.62 -0.81), but the correlation with unsweetened ethanol solutions was considerably lower (rs = 0.37 -0.45). These results add new strains to the strain mean database that will facilitate the identification of genetic relationships between voluntary ethanol consumption, saccharin preference, and other phenotypes.

show abstract

“…Electrophysiologically, ethanol has been shown to have a direct and indirect effect to potentiate GABA A receptor function, with the indirect effect related to an increase in steroidogenesis (Sanna et al, 2004). This finding is consistent with reports that acute exposure to ethanol by injection or consumption in rodents (Barbaccia et al, 1999;Finn et al, 2004b;Morrow et al, 1999;VanDoren et al, 2000) or consumption in human adolescents (Torres & Ortega, 2003 significantly increased endogenous ALLO levels to pharmacologically active concentrations. The fact that the 5α-reductase inhibitor finasteride (which decreases endogenous ALLO levels) reduced sensitivity to some, but not all, effects of ethanol (e.g., Dazzi et al, 2002;Gabriel et al, 2004;Hirani et al, 2002Hirani et al, , 2005Khisti et al, 2004;Murphy et al, 2006;VanDoren et al, 2000) suggests that there is a complex interaction between ALLO and ethanol in biological systems.…”

Section: Introductionsupporting

confidence: 87%

Decreased anticonvulsant efficacy of allopregnanolone during ethanol withdrawal in female Withdrawal Seizure-Prone vs. Withdrawal Seizure-Resistant mice

Beckley¹,

Fretwell²,

Tanchuck³

et al. 2008

Neuropharmacology

View full text Add to dashboard Cite

SUMMARYThe GABAergic neurosteroid allopregnanolone (ALLO) has been repeatedly shown to have an increased anticonvulsant effect during ethanol withdrawal in rats and in C57BL/6J mice. In contrast, the seizure prone DBA/2J inbred strain and the Withdrawal Seizure-Prone (WSP) selected line exhibited decreased sensitivity to ALLO's anticonvulsant effect during ethanol withdrawal, with no change in sensitivity in the Withdrawal Seizure-Resistant (WSR) line. To date, only male mice have been tested. Thus, the present study examined ALLO sensitivity during ethanol withdrawal in female WSP and WSR mice, since females display less severe physical symptoms of withdrawal and have higher circulating ALLO levels than males. Female WSP and WSR mice were exposed to ethanol vapor or air for 72 hr. During peak ethanol withdrawal, separate groups of mice were injected with vehicle or ALLO (0, 3.2, 10, or 17 mg/kg, ip) prior to the timed tail vein infusion of pentylenetetrazol (PTZ). ALLO injection significantly increased the threshold dose for onset to PTZ-induced convulsions, indicating an anticonvulsant effect, in female WSP and WSR mice. During ethanol withdrawal, sensitivity to ALLO's anticonvulsant effect was slightly increased in female WSR mice but was significantly decreased in female WSP mice. This line difference in sensitivity to ALLO during ethanol withdrawal in female mice was similar to that in the male mice. Notably, all seizure prone genotypes tested to date displayed tolerance to the anticonvulsant effect of ALLO during ethanol withdrawal, suggesting that decreased sensitivity of GABA A receptors to ALLO may contribute to the increased ethanol withdrawal phenotype.

show abstract

Sex differences in the effect of ethanol injection and consumption on brain allopregnanolone levels in C57BL/6 mice

Cited by 85 publications

References 21 publications

Allopregnanolone influences the consummatory processes that govern ethanol drinking in C57BL/6J mice

Allopregnanolone influences the consummatory processes that govern ethanol drinking in C57BL/6J mice

Voluntary ethanol consumption in 22 inbred mouse strains

Decreased anticonvulsant efficacy of allopregnanolone during ethanol withdrawal in female Withdrawal Seizure-Prone vs. Withdrawal Seizure-Resistant mice

Contact Info

Product

Resources

About