Sex hormone profiles and cellular changes of reproductive organs of mice experimentally infected with C. pseudotuberculosis and its exotoxin phospholipase D (PLD)

Khuder, Zaid

doi:10.9790/2380-0132429

Cited by 16 publications

(17 citation statements)

References 20 publications

(19 reference statements)

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“…Furthermore, the lower concentrations of estrogen induced by both inoculation of P. multocida type B: 2 and its LPS in the female mice seems to decrease the susceptibility of the female mice to infections, this could be due to the correlations between the decrease in the concentration of estrogen and the levels of the severity in the histopathological alterations in the pituitary glands and the ovaries of the female mice. This finding was also similar to the study conducted by (Khuder et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

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Oral infections of mice as animal model with Pasteurella multocida type B: 2 and its lipopolysaccharides is liable to cause detrimental alterations in the pituitary glands, which results in disruption of reproductive hormonal levels as well as organ functions in the male and female animals; thereby impeding the fertility of at risk animal population. Therefore, this study was designed to evaluate the histopathological modifications in the pituitary glands, reproductive organs and the associated hormonal levels in male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharides. Forty eight mice were divided into three groups consisting of sixteen mice each (8 male and 8 female in separate cages). Group 1 served as the control group and were inoculated orally with 0.4 mL Phosphate Buffer Saline (PBS), group 2 were inoculated orally with 0.4 mL of 10 9 Colony Forming Unit (CFU) of P. multocida type B: 2 while group 3 were inoculated orally with 0.4 mL of LPS from 10 9 of colony P. multocida type B: 2. There were significant differences (p<0.05) in the lesions (oedema, haemorrhage, degeneration and necrosis) observed in the testes, ovaries and the pituitary glands amongst the three groups. Similarly, there were significant differences in the levels of testosterone, progesterone and estrogen (p<0.05) in the male and female mice inoculated orally with P. multocida type B: 2 and its lipopolysaccharide. There is paucity of information on changes related to reproductive hormones, pituitary glands and reproductive organs of male and female mice following oral inoculation with P. multocida type B: 2 and its endotoxin. Hence, the sex hormonal levels could be used to assess the immune statuses of at risk animal population and proffer amenable Veterinary medical care to afflicted animal population.

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Section: Discussionsupporting

confidence: 92%

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Abdullah¹,

Adamu²,

Tijjani³

et al. 2015

American Journal of Animal and Veterinary Sciences

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“…At the same time, the LPS may have direct effects on the cells and may cause septicemic phase. Similar results were also obtained by Khuder et al (2012) in female mice inoculated with Corynebacterium pseudotuberculosis, and the main changes were related to septicemia. These changes included congestion, infiltration of neutrophil and macrophages, degeneration, necrosis, haemorrhage and oedema.…”

Section: Discussionsupporting

confidence: 88%

Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)

et al. 2015

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Pasteurella multocida is responsible for one of the major animal diseases with economic importance in both developed and developing countries. P. multocida B:2 causes haemorrhagic septicemia (HS) in cattle and buffaloes, which result in heavy economic losses through direct effect, resulting in high mortality rates and indirect effects through impairment of the animal productivity. It is well known that HS affects mainly the respiratory and digestive tracts of host animals. However, involvement of the reproductive system in the pathogenesis of HS has not been reported previously. This study was designed to present detailed detection and histopathological changes of the reproductive system and mammary glands of buffaloes experimentally infected with P. multocida B:2. Briefly, nine healthy buffalo calves aged 8-month-old were divided into three groups of three calves each. Calves from groups 1 and 2 were inoculated subcutaneously and orally with 10 mL of 1×10 12 cfu/mL of P. multocida B:2, respectively, while calves of group 3 were inoculated orally with 10 mL of phosphate buffer saline as a sham control. All the calves in group 1 had to be euthanized after 12 h post-infection, while calves in group 2 were euthanized after day 21 post-infection. Successful isolation and PCR confirmation of P. multocida B:2 was achieved from different parts of the reproductive system, including ovary, oviduct, uterine horn, uterine body and vagina as well as mammary glands and supramammary lymph nodes of the buffaloes in group 1 after 12 h post-infection. However, P. multocida B:2 was not detected in the reproductive organs of buffaloes in group 2 and group 3 after 21 days post-infection. Significant differences (P<0.05) were found in histopathological changes between the two groups, which mainly affected different anatomic regions of the reproductive system. This work provides an insight into the involvement of the female reproductive system of buffaloes during the pathogenesis of HS and shows that route of inoculation strongly affect the localization of the bacterium in the reproductive system.

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“…C. pseudotuberculosis is the causative agent of CLA in ruminants [ 12 , 13 ]. Although the bacterium causes low mortality, but it may influences the fertility of the infected animal, which may lead to major economic losses [ 14 ]. Infertility is one of the major problem dealt by goat industry where anything interferences with the reproductive system will lead to an infertile animal [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The presence of this organism in the reproductive organs of the infected does after 60 and 90 days post-infection may cause infertility where according to Jesse et al . (2014), stated that presence of the bacterium in the reproductive organs may alter the secretion of female reproductive hormone [ 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form

Latif¹,

Abdullah²,

Othman³

et al. 2015

Vet World

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Aim:Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis that affects sheep and goats. This study was designed to determine the presence of the causative organism in the female reproductive organs and associated lymph nodes in non-pregnant does experimentally inoculated through intradermal route in the chronic form.Materials and Methods:18 non-pregnant healthy Katjang does aged 2-year-old were divided randomly into two groups. The first and second group consists of nine non-pregnant does each and the two groups were subdivided into three subgroups. The first group was experimentally inoculated with 1 ml of 107cfu of live C. pseudotuberculosis through intradermal route, whereas the second group was inoculated with 1 ml phosphate buffer saline (pH 7) solution intradermally. The first group were further subdivided into three subgroups where, the first subgroup (B1) were kept for 30 days post-infection, second subgroup (B2) were kept for 60 days post-infection, and third subgroup (B3) were kept for 90 days. The second group was further subdivided into three subgroups (C1, C2, and C3) where they were kept for 39, 60, and 90 days post-infection, respectively.Results:From this study, there was successful isolation of C. pseudotuberculosis from the reproductive organs of the treatment group after 60 days post-infection. The subgroups (B1, C1, C2, and C3) did not show any presence of the causative organism in the reproductive organs. The second subgroup B2 and third subgroup B3 showed positive isolation of the causative organisms from the ovary, uterine horns, uterus, cervix, vagina, and inguinal lymph node of the experimental non-pregnant does.Conclusion:This study showed that chronic infection of C. pseudotuberculosis via intradermal route may cause effect toward the reproductive organs and may be able to influence the reproductive efficiency of the infected animals.

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Sex hormone profiles and cellular changes of reproductive organs of mice experimentally infected with C. pseudotuberculosis and its exotoxin phospholipase D (PLD)

Cited by 16 publications

References 20 publications

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Hormonal and Histopathological Alterations in Pituitary Glands and Reproductive Organs of Male and Female Mice Orally Inoculated with <i>Pasteurella Multocida</i> Type B: 2 and its Lipopolysaccharides

Molecular detection and pathology of Pasteurella multocida B:2 in the reproductive system of pre-pubertal buffalo calves (Bubalus bubalis)

Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form

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