Sex in Penicillium: Combined phylogenetic and experimental approaches

López‐Villavicencio, Manuela; Aguileta, Gabriela; Giraud, Tatiana; Vienne, Damien M. de; Lacoste, Sandrine; Couloux, Arnaud; Dupont, Joëlle

doi:10.1016/j.fgb.2010.05.002

Cited by 38 publications

(30 citation statements)

References 63 publications

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“…(Lopez- Villavicencio et al 2010). In addition, the 3 0 untranslated region (3 0 UTR) of P. marneffei areA contains a highly conserved sequence present in areA orthologues from Aspergillus and Penicillium species, which influences areA mRNA stability (Supp.Fig 2;Platt et al 1996;Morozov et al 2001).…”

mentioning

confidence: 99%

AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

2012

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mentioning

confidence: 99%

AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

2012

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“…Amplification and sequencing of the mating type genes confirmed that T. flavovirens is heterothallic, with individual isolates containing either the MAT1 or MAT2 genes, never both. A recent paper reported the development of a sexual stage for T. pinophilus (López-Villavicencio et al 2010), a close relative of T. flavovirens, induced only after media dried out. The similarity with the arid habitat of T. flavovirens is interesting, but in our experiments slow drying of the colonies did not induce maturation of the putative ascoma initials.…”

Section: Discussionmentioning

confidence: 99%

“…Further, the strains DAOM 236381-236383 and IBT 27044 were crossed in all possible combinations on OA, and on 2% water agar with Q. macrocarpa leaves. After several months of incubation at RT under ambient light, the Parafilm was removed to allow the agar to dry out (López-Villavicencio et al 2010), in an attempt to mimic the species' arid natural habitat.…”

Section: Teleomorph-anamorph Connection and Mating Experimentsmentioning

confidence: 99%

“…Mating-type genes were amplified from all strains using the primers MAT1-1b and MAT1-2b with a touchdown PCR (López-Villavicencio et al 2010). The PCR profile had an initial denaturing step at 95°C for 5 min, followed by 30 cycles of denaturing at 95°C for 30 sec, annealing starting at 60°C (decreased -0.5°C for every cycle until 50°C) for 30 sec, elongation at 72°C for 1 min, followed by a final elongation step at 72°C for 7 min (López-Villavicencio et al 2010).…”

Section: Teleomorph-anamorph Connection and Mating Experimentsmentioning

confidence: 99%

“…The PCR profile had an initial denaturing step at 95°C for 5 min, followed by 30 cycles of denaturing at 95°C for 30 sec, annealing starting at 60°C (decreased -0.5°C for every cycle until 50°C) for 30 sec, elongation at 72°C for 1 min, followed by a final elongation step at 72°C for 7 min (López-Villavicencio et al 2010). To confirm the presence or absence of the MAT1 and MAT2 genes, products were run on a 1% agarose gel.…”

Section: Teleomorph-anamorph Connection and Mating Experimentsmentioning

confidence: 99%

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Phylogenetic relationships and the newly discovered sexual state of Talaromyces flavovirens, comb. nov.

Visagie¹,

Llimona²,

Vilà³

et al. 2013

mycotaxon

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-Typical Talaromyces ascomata were observed on dry Quercus suber leaf litter amongst the characteristic synnemata of Penicillium aureocephalum, and they appear to represent the sexual state of the latter species. The species is a synonym of the older Lasioderma flavovirens, and we propose the new combination Talaromyces flavovirens. Lectotype and epitype specimens are designated for this name. The defining characters of the asexual state include yellow, short-stalked, mycetozoan-like synnemata with an unusual, almost closed terminal head of penicillate conidiophores intermixed with sinuous hyphae, and dark green conidia. Ascomata could not be induced in culture, but PCR amplifications of mating-type genes indicate the species is heterothallic. In nature, ascocarp initials appear to be antheridia coiled around clavate ascogonia, similar to those of T. flavus, and the thick-walled, spiny ascospores are also similar to those of T. flavus. ITS barcodes and β-tubulin sequences place T. flavovirens in a clade with T. apiculatus, T. flavus, T. funiculosus, T. galapagensis, T. pinophilus, T. macrosporus, and seven other species.

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Induction of the Sexual Cycle in Filamentous Ascomycetes

Houbraken

Dyer

2014

Fungal Biology

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The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

show abstract

Sex in Penicillium: Combined phylogenetic and experimental approaches

Cited by 38 publications

References 63 publications

AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

Phylogenetic relationships and the newly discovered sexual state of Talaromyces flavovirens, comb. nov.

Induction of the Sexual Cycle in Filamentous Ascomycetes

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