Sex ratios in mule duck embryos at various stages of incubation

Batellier, Florence; Marchal, Francis; Scheller, Marie; Gautron, Joël; Sellier, Nadine; Taouis, Mohammed; Monbrun, C.; Vignal, Alain; Brillard, Jean-Pierre

doi:10.1016/s0093-691x(03)00208-5

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…However, several studies reported that hatching success in mule ducks was generally measured at between 70 and 80% of fertile eggs (Brun et al, 2005;Marie-Etancelin et al, 2008), which implies that even though this capacity is clearly affected by heat treatment, hatching performance remains in the same range (at between 75 and 82%). Concerning the sex ratio, TM did not change the imbalance induced by mule hybrid generation, leading to an increase in female embryonic mortality (Batellier et al, 2004).…”

Section: Early Impact Of Embryonic Tmmentioning

confidence: 78%

Positive Impact of Thermal Manipulation During Embryogenesis on Foie Gras Production in Mule Ducks

Massimino

Davail

Bernadet³

et al. 2019

Front. Physiol.

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Section: Early Impact Of Embryonic Tmmentioning

confidence: 78%

Positive Impact of Thermal Manipulation During Embryogenesis on Foie Gras Production in Mule Ducks

Massimino

Davail

Bernadet³

et al. 2019

Front. Physiol.

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“…For each embryo, primary cells were obtained after trypsinization of the embryo tissues and the rest of the tissues were stored for DNA extraction. Duplex PCR was performed to test the sex of embryos according to Batellier et al [62]. Fibroblasts from only one female embryo were propagated in complete DMEM medium (DMEM Glutamax (Gibco Co.) supplemented with 10% foetal calf serum (Gibco Co.), 1% penicillin and streptomycin) at 40°C with 5% CO 2 and used as donor cells.…”

Section: Methodsmentioning

confidence: 99%

A duck RH panel and its potential for assisting NGS genome assembly

et al. 2012

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BackgroundOwing to the low cost of the high throughput Next Generation Sequencing (NGS) technology, more and more species have been and will be sequenced. However, de novo assemblies of large eukaryotic genomes thus produced are composed of a large number of contigs and scaffolds of medium to small size, having no chromosomal assignment. Radiation hybrid (RH) mapping is a powerful tool for building whole genome maps and has been used for several animal species, to help assign sequence scaffolds to chromosomes and determining their order.ResultsWe report here a duck whole genome RH panel obtained by fusing female duck embryonic fibroblasts irradiated at a dose of 6,000 rads, with HPRT-deficient Wg3hCl2 hamster cells. The ninety best hybrids, having an average retention of 23.6% of the duck genome, were selected for the final panel. To allow the genotyping of large numbers of markers, as required for whole genome mapping, without having to cultivate the hybrid clones on a large scale, three different methods involving Whole Genome Amplification (WGA) and/or scaling down PCR volumes by using the Fluidigm BioMarkTM Integrated Fluidic Circuits (IFC) Dynamic ArrayTM for genotyping were tested. RH maps of APL12 and APL22 were built, allowing the detection of intrachromosomal rearrangements when compared to chicken. Finally, the panel proved useful for checking the assembly of sequence scaffolds and for mapping EST located on one of the smallest microchromosomes.ConclusionThe Fluidigm BioMarkTM Integrated Fluidic Circuits (IFC) Dynamic ArrayTM genotyping by quantitative PCR provides a rapid and cost-effective method for building RH linkage groups. Although the vast majority of genotyped markers exhibited a picture coherent with their associated scaffolds, a few of them were discordant, pinpointing potential assembly errors. Comparative mapping with chicken chromosomes GGA21 and GGA11 allowed the detection of the first chromosome rearrangements on microchromosomes between duck and chicken. As in chicken, the smallest duck microchromosomes appear missing in the assembly and more EST data will be needed for mapping them. Altogether, this underlines the added value of RH mapping to improve genome assemblies.

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“…This finding is not at all surprising since skewed sex ratio values have been yet reported in other water birds as well as in Anas platyrhynchos (Bellrose et al 1961;Blums & Mednis 1996;Batellier et al 2004). The reason may be due to many factors, such as sex-differential mortality at or after hatching or the lower visibility during egg deposition of females since they hide in the riparian vegetation.…”

Section: Discussionmentioning

confidence: 55%

“…For both the rivers a significant difference in sex ratio values among seasons (observed vs. observed) was recorded (Arno: G5168.8, df53, P,0.001; Mugnone, G585.38, df53, P,0.001: G test): the highest value for both rivers is that recorded during the summer, while the lowest occur in the autumn (Table II). We then tested the null hypothesis of a sex ratio not different from 50% (observed versus expected), as indicated by genetic analyses carried out on 1-5-day-old mallard embryos (Batellier et al 2004). Most of the tertiary (adult) sex ratio values (with the exception of winter and autumn Mugnone values) are strongly skewed in favour of males (Table II).…”

Section: Ecological Observationsmentioning

confidence: 99%

Molecular and ecological characterization of urban populations of the mallard (Anas platyrhynchosL.) in Italy

Baratti

Cordaro

Dessì‐Fulgheri

et al. 2009

Italian Journal of Zoology

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Urban habitats are increasing worldwide and represent new potential environments for many species of animals, in particular birds. As a result of contact with humans, urban wildlife can experience ecological and ethological modifications, mainly regarding parental and feeding behaviour. One of the most common birds in urban landscapes is the mallard, Anas platyrhynchos L., which is widespread in temperate regions. This study aimed to characterize two Italian populations of mallards living in a metropolitan area, from both an ecological and a genetic point of view. Field surveys were carried out for 27 consecutive months along two rivers, focusing on the biological cycle and reproductive behaviour of the mallard. The ecological results were integrated with molecular data, obtained by the analysis of 10 microsatellites, genotyped in the two urban, in a wild and in a captive-bred population. No significant changes in eco-ethological features of the mallards living in the urban area were observed, whereas the urban populations were genetically differentiated from the wild and the bred ones.

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Sex ratios in mule duck embryos at various stages of incubation

Cited by 8 publications

References 13 publications

Positive Impact of Thermal Manipulation During Embryogenesis on Foie Gras Production in Mule Ducks

Positive Impact of Thermal Manipulation During Embryogenesis on Foie Gras Production in Mule Ducks

A duck RH panel and its potential for assisting NGS genome assembly

Molecular and ecological characterization of urban populations of the mallard (Anas platyrhynchosL.) in Italy

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