Sex-specific Metabolic Characterization of Serum and Liver of Mice with High-fat Diet

Abstract

Background: Obesity exhibit sex differences is well established, but its metabolic mechanism remains unclear. Thus, investigation of metabolic pattern of male and female mice with high-fat diet (HFD) is of substantial importance for explore the potential mechanism linking gender differences in obesity.Methods: In the present study, we analyzed the metabolic changes in serum and liver of male and female mice with high-fat diet using nuclear magnetic resonance-based metabolomic approach.Results: Principle component analysis show that the metabolic pattern of serum and liver of male mice with HFD was significantly distinguished from the other groups. Furthermore, the accumulation of low-density lipoprotein/very low-density lipoprotein was found in the serum of male mice with HFD. Moreover, metabolomic results of liver reveal that tricarboxylic acid cycle and amino acid metabolism are increased in female mice with HFD.Conclusion: In conclusion, our results suggest that the differences in energy and amino acid metabolism of males and females were most likely influence the predisposition to obesity.