Sexing two-week old bovine embryos by chromosomal analysis prior to surgical transfer: Preliminary methods and results

Hare, W.C.D.; Mitchell, David; Betteridge, K.; Eaglesome, M.D.; Randall, G. C. B.

doi:10.1016/0093-691x(76)90236-3

Cited by 69 publications

(20 citation statements)

References 3 publications

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“…They demonstrate that most of the ES cell lines (70-90%) possessed a normal and stable karyotype Robertson et al 1983). The chromosomal configuration in our study is identical to previous reports for bovine embryos (Hare et al 1976;King et al 1979;Wintenberger-Torres and Popescu 1980).…”

Section: Resultssupporting

confidence: 80%

Bovine embryonic stem cell-like cell lines cultured over several passages

Saito

Strelchenko

Niemann

1992

Roux's Arch Dev Biol

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A total of 14 microsurgically produced zona pellucida-free bovine demi-blastocysts were cultured for 3 days in tissue culture medium (TCM) 199 supplemented with 10% heat-inactivated newborn calf serum (NBCS). Developing embryos were continuously cultured in TCM 199 plus 10% NBCS on a feeder-layer of murine embryonic fibroblasts, that had been incubated with mitomycin C (10 μg/ml) for 3 h prior to the onset of embryo cultivation to block mitotic activity of the fibroblasts. After 2 days, 3 expanded blastocysts were attached to the feeder-layer and both trophoblastic cells and inner cell mass (ICM) cells became apparent on the 9th day of culture in 2 out of the 3 expanded blastocysts. Five days later, the ICM cells were disaggregated by a short-term trypsin treatment. The resulting dissociated clumps were seeded on a new murine embryonic fibroblast feeder-layer and covered with modified minimum essential medium (MEM)-Alpha with 10% fetal calf serum (FCS), 0.1 mm mercaptoethanol, 4.5 g/l glucose and 20 mM HEPES-buffer (=passage 0). To prevent differentiation of the cells, approximately 1/3 of the MEM-Alpha was replaced by MEM previously incubated on cell line 5637 containing leucaemia inhibitory factor (LIF) for 3 days. Colonies of embryonic stem cell (ES)-like cells were observed 5 days after the 1st passage. These colonies were repeatedly passaged at approximately 2-week intervals. Two bovine ES-like cell lines were established, which grew considerably slower than murine ES cells, but were lost after the 4th passage, possibly because of toxic effects of a new FCS batch. After cytogenetic analysis, 16 out of 18 metaphase plates contained an euploid number of chromosomes with 2 X-chromosomes and 58 autosomes. Distribution of G-banding on the chromosomes of ES-like cells was in accordance with the diploid set of the bovine genome. ES-like cells were fused to in vitro matured bovine oocytes and, upon successful fusion, cultured in vitro over 5 days. Successful fusion was observed in 79.8% (67/84), 31.3% initiated cleavege and 10.4% reached the 8-16 cell stage at termination of culture.

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Section: Resultssupporting

confidence: 80%

Bovine embryonic stem cell-like cell lines cultured over several passages

Saito

Strelchenko

Niemann

1992

Roux's Arch Dev Biol

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“…It is now possible to collect and transfer embryos using non-surgical techniques (Sreenan, 1975;Testart & Godard-Siour, 1975;Brand, Drost, Aarts & Gunnink, 1976;Elsden, Hasler & Seidel, 1976;Rowe, Del Campo, Eilts, French, Winch & Ginther, 1976). Furthermore, embryos can be sexed before being transferred (Hare, Mitchell, Betteridge, Eaglestome & Randall, 1976) and can be stored for years by deep freezing (Wilmut & Rowson, 1973;Bilton & Moore, 1976;Willadsen, Trounson, Polge, Rowson & Newcomb, 1976;Willadsen, Polge & Rowson, 1978). However, even though studies on superovulation in cattle began 40 years ago (Zavadowski & Eskin, 1939), the variability of responses in quantity and quality is still so large that it is now the limiting factor in the development of practical embryo transfer programmes in cattle breeding.…”

Section: Introductionmentioning

confidence: 99%

CONCENTRATIONS OF LUTEINIZING HORMONE, OESTRADIOL-17β AND PROGESTERONE IN THE PLASMA OF HEIFERS TREATED TO INDUCE SUPEROVULATION

Saumande¹

1980

Journal of Endocrinology

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Concentrations of luteinizing hormone (LH), oestradiol-17beta and progesterone were studied in 14 heifers treated with pregnant mare serum gonadotrophin (PMSG) and an analogue of prostaglandin F2alpha (PGF2alpha) 48 h later to induce superovulation. Plasma levels of oestradiol-17beta before ovulation and of progesterone after ovulation were related to the number of ovulations but the concentrations of LH were not so related; however, hormone concentrations at the time of injection of PMSG could not be used to predict the rate of ovulation. The injected gonadotrophin also appeared to affect folliculogenesis after ovulation and this may explain the presence of large follicles in the ovary when the corpora lutea were counted. It was possible to demonstrate by analysis of plasma levels of hormones that few non-superovulated animals were really insensitive to PMSG.

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“…(Hare et al, 1976), which appears to be much less viable after freezing and storage than the week-old embryo . (Hare et al, 1976), which appears to be much less viable after freezing and storage than the week-old embryo .…”

Section: Frozen Cattle Embryosmentioning

confidence: 95%

Embryo transfer and twinning in cattle

Gordon

Boland

1979

Vet Res Commun

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Gordon, I. and Boland, M.P., 1979. Embryo transfer and twinning in cattle. Vet. Sci. Commun., 3: 177-186.Recent developments in cattle embryo transfer are discussed, particularly those that are relevant to the commercial application of the technique.Cattle twinning for beef production could offer impressive economic advantages where nutrition is not a limiting factor and intensive management is possible.Evidence is discussed which suggests that cattle twins induced by embryo transfer may hold several advantages over naturally-occurring twins.

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Sexing two-week old bovine embryos by chromosomal analysis prior to surgical transfer: Preliminary methods and results

Cited by 69 publications

References 3 publications

Bovine embryonic stem cell-like cell lines cultured over several passages

Bovine embryonic stem cell-like cell lines cultured over several passages

CONCENTRATIONS OF LUTEINIZING HORMONE, OESTRADIOL-17β AND PROGESTERONE IN THE PLASMA OF HEIFERS TREATED TO INDUCE SUPEROVULATION

Embryo transfer and twinning in cattle

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