This study aims to determine the relationship between oestrus character and the success of double dosage AI at the 2 and 8 h AI intervals in Madura cattle. The research was conducted in Lenteng, Sumenep Regency. The material used was 45 cows Madura cattle which were selected by purposive sampling. The semen used was frozen semen from male Madura cattle produced by the Singosari Center for Artificial Insemination (SCAI), Malang. The method used in this study was observational; namely, the primary data collection was done by direct observation, including oestrus, signs of heat, feed, and direct interviews with farmers. At the same time, secondary data on livestock reproduction was obtained from data recording by inseminator officers. The results showed that the best percentage of red vulva color was NRR-1 93.33%, NRR-2 88.89%, CR 77.78%, and PR 82.22%. The percentage of vaginal temperature > 38 ºC is the value of NRR-1 and NRR-2 68.89%, CR 64.44%, and PR 68.89%. The pH indicator value of 8 showed the percentage of NRR-1 75.56%, NRR-2 71.00%, CR 62.00%, and PR 66.67%. There are many conditions of cervical mucus, namely NRR-1 48.89%, NRR-2 44,44%, CR 46.67%, and PR 48.89%. The categories of vulvar swelling were very swollen NRR-1 62.22%, NRR-2 60.00%, CR 55.56%, and PR 60.00%. Vaginal smear values for the oestrus category were NRR-1 84.00%, NRR-2 82.22%, CR 68.00%, and PR 70.00%. In conclusion, A high percentage of pregnancy was indicated in oestrus conditions with an evenly red vulva color, temperature > 38 ºC, a pH value of 8, and there was much mucus in the condition, the vulva had a very swollen swelling value, and a vaginal smear value in the oestrus category.