1992
DOI: 10.1016/0147-5975(92)90039-t
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Sexual crosses of the homothallic fungusGaeumannomyces graminis var.tritici based on use of an auxotroph obtained by transformation

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Cited by 20 publications
(16 citation statements)
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“…However, intraisolate crosses have been shown to occur in vitro (Zadocks et al, 1974). Ggt genotypic frequencies and disease severity 495 (Pilgeram and Henson, 1992) and sexual recombination could sometimes take place between different strains in Gg natural populations (Bryan et al, 1999). But the generation of ascospores on the base of stems at the end of the cropping season is a rare event only observed after a severe outbreak of the disease under favourable climatic conditions and doubt persists about the infectivity of the generated ascospores (Asher, 1981).…”
Section: Discussionmentioning
confidence: 99%
“…However, intraisolate crosses have been shown to occur in vitro (Zadocks et al, 1974). Ggt genotypic frequencies and disease severity 495 (Pilgeram and Henson, 1992) and sexual recombination could sometimes take place between different strains in Gg natural populations (Bryan et al, 1999). But the generation of ascospores on the base of stems at the end of the cropping season is a rare event only observed after a severe outbreak of the disease under favourable climatic conditions and doubt persists about the infectivity of the generated ascospores (Asher, 1981).…”
Section: Discussionmentioning
confidence: 99%
“…Because G. graminis is homothallic (self‐fertile), attempts to perform genetic crosses often result in relatively small proportions of hybrid perithecia compared with the numbers that result from selfing of each parental strain (Asher, 1981; Blanch et al ., 1981). In intra‐isolate crosses, using mutants or antibiotic‐resistant transformants, the percentage of hybrid perithecia was generally around 10–25% but sometimes higher frequencies were obtained (Hornby et al ., 1998; Musker, 1994; Pilgeram and Henson, 1992). Also, numerous vegetative incompatibility groups exist within G. graminis (Asher, 1981; Jamil and Buck, 1991; Jamil et al ., 1984) and this places restrictions on the isolate combinations that can be sucessfully paired.…”
Section: Genetics and Transformation Of G Graminismentioning
confidence: 99%
“…The stability of the integrated DNA varies. Transgenes in the majority of transformants are mitotically stable, but their stability through meiosis varies with copy number and with different genes (Pilgeram and Henson, 1990, 1992; Pilgeram et al ., 1993). For example, benomyl‐ and phleomycin‐resistant transformants of Ggt and Ggg were mitotically stable irrespective of transgene copy number.…”
Section: Genetics and Transformation Of G Graminismentioning
confidence: 99%
“…In a few cases, tagged mutations have been generated by random insertion, during transformation, of a plasmid that has no homology with the fungal genome (4)(5)(6)(7). This procedure, however, is generally inefficient because the frequency of transformation with nonhomologous plasmids is often low.…”
mentioning
confidence: 99%