Sexual Recombination in <i>Gibberella zeae</i>

Bowden, Robert L.; Leslie, John F.

doi:10.1094/phyto.1999.89.2.182

Cited by 238 publications

(170 citation statements)

References 32 publications

Supporting

Mentioning

167

Contrasting

Order By: Relevance

“…Similarity matrix generated through Jacquard coefficient. Bowden and Leslie (1999). They reported that sexual reproduction is considered as an important assumption for molecular divergence among the population from different geographical regions.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India

Sharma¹,

P²,

S³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most serious disease and causes damage to oilseed Brassica. Seventeen geographical isolates of S. sclerotiorum from different geographical locations of India were compared for their growth under artificial culture conditions and the result was validated through random amplified polymorphic DNA (RAPD) markers. Based on the morphological studies, the isolates were grouped into fast growing (SR-01, SR-02, SR-05, SR-16) and intermediate (SR-03, SR-04, SR-07, SR-10 to SR-13, SR-18), though the rest of the isolates showed comparatively a slow mycelial growth rate. In RAPD analysis for their genetic diversity, fifty decamer primers of arbitrary sequences produced 692 scorable amplicons ranging from 180 to 3900 bp in size. Out of these, 385 fractionated fragments were reported polymorphic. As an average, 13-14 unambiguous and reproducible amplified products were generated by single primer (minimum of 4 and maximum of 23 fragments). Hence, the present investigation confirmed the diversity among the different geographical isolates from India.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India

Sharma¹,

P²,

S³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…This strain was derived from the same parental strain (CS3005) as the TRI5-GFP strain (see Table 1 for a summary of the fungal strains used in this study). Crossing was performed on carrot agar plates as described elsewhere (Bowden and Leslie, 1999). Ascospores were harvested from the Petri plate lid and spread on to ½ PDA plates containing 50 mg L -1 G418 and 200 mg L -1 hygromycin.…”

Section: Backcrossing and Genotypingmentioning

confidence: 99%

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Blum

Benfield

Stiller

et al. 2016

Fungal Genetics and Biology

View full text Add to dashboard Cite

., High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production, Fungal Genetics and Biology (2016), doi: http://dx.doi. org/10.1016/j.fgb. 2016.02.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACTFusarium head blight and crown rot, caused by the fungal plant pathogen Fusarium graminearum, impose a major threat to global wheat production. During the infection, plants are contaminated with mycotoxins such as deoxynivalenol (DON), which can be toxic for humans and animals. In addition, DON is a major virulence factor during wheat infection. However, it is not fully understood how DON production is regulated in F. graminearum. In order to identify regulators of DON production, a high-throughput mutant screen using Fluorescence Activated Cell Sorting (FACS) of a mutagenized TRI5-GFP reporter strain was established and a mutant over-producing DON under repressive conditions identified. A gain-of-function mutation in the F. graminearum adenylyl cyclase (FAC1), which is a known positive regulator of DON production, was identified as the cause of this phenotype through genome sequencing and segregation analysis. Our results show that the high-throughput mutant screening procedure developed here can be applied for identification of fungal proteins involved in diverse processes.

show abstract

“…The F. graminearum wild-type strain, Z-3639 (Bowden & Leslie, 1999), and mutants derived from this strain were used in this study and are listed in Table 1. Conidial suspensions or agar blocks containing mycelia of each strain were stored in 20 % glycerol at 270 uC.…”

Section: Methodsmentioning

confidence: 99%

A novel gene, GEA1, is required for ascus cell-wall development in the ascomycete fungus Fusarium graminearum

Son

Lee

2013

Microbiology

View full text Add to dashboard Cite

Sexual Recombination in Gibberella zeae

Cited by 238 publications

References 32 publications

Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India

Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

A novel gene, GEA1, is required for ascus cell-wall development in the ascomycete fungus Fusarium graminearum

Contact Info

Product

Resources

About