Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grasses

Sonawane, Balasaheb V.; Sharwood, Robert E.; Whitney, Spencer M.; Ghannoum, Oula

doi:10.1093/jxb/ery129

Cited by 48 publications

(76 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oxidoreductase activity is often linked with the cyclic electron flow (CEF) around photosystem I (Takabayashi et al , ), and NADP‐ME species have been reported to utilize the highest amount of CEF (Hernández‐Prieto et al , ). Plasticity within CEF could have implications in the ability to acclimate to low light, and the subtype‐specific nature of this plasticity could have implications in the lower susceptibility of NADP‐ME species to shade that has previously been reported (Sonawane et al , ). Within NAD‐ME species an increased enrichment in categories related to starvation was noted within the downregulated DE transcripts.…”

Section: Discussionmentioning

confidence: 90%

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

et al. 2019

Self Cite

View full text Add to dashboard Cite

Summary Three subtypes of C4 photosynthesis exist (NADP‐ME, NAD‐ME and PEPCK), each known to be beneficial under specific environmental conditions. However, the influence of photosynthetic subtype on transcriptomic plasticity, as well as the genes underpinning this variability, remain largely unknown. Here, we comprehensively investigate the responses of six C4 grass species, spanning all three C4 subtypes, to two controlled environmental stresses: low light (200 µmol m−2 sec−1) and glacial CO2 (subambient; 180 ppm). We identify a susceptibility within NADP‐ME species to glacial CO2. Notably, although glacial CO2 phenotypes could be tied to C4 subtype, biochemical and transcriptomic responses to glacial CO2 were largely species specific. Nevertheless, we were able to identify subtype specific subsets of significantly differentially expressed transcripts which link resource acquisition and allocation to NADP‐ME species susceptibility to glacial CO2. Here, low light phenotypes were comparable across species with no clear subtype response, while again, transcriptomic responses to low light were largely species specific. However, numerous functional similarities were noted within the transcriptomic responses to low light, suggesting these responses are functionally relatively conserved. Additionally, PEPCK species exhibited heightened regulation of transcripts related to metabolism in response to both stresses, likely tied to their C4 metabolic pathway. These results highlight the influence that both species and subtype can have on plant responses to abiotic stress, building on our mechanistic understanding of acclimation within C4 grasses and highlighting avenues for future crop improvements.

show abstract

Section: Discussionmentioning

confidence: 90%

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…NADP-ME genes can be classified into photosynthetic and non-photosynthetic types. The former mostly function in the chloroplasts [13] and improve photosynthetic efficiency by facilitating the release of CO 2 from decarboxylation of malate in proximal bundle-sheath cells, and in C 4 plants by providing CO 2 to Rubisco for carbon fixation [14,15]. Genomic and phylogenetic analyses showed that the NADP-ME gene family in the Poaceae has four branches, with one branch (NADP-ME IV) being expressed in the plastids.…”

Section: Introductionmentioning

confidence: 99%

Identification and evolution of C4 photosynthetic pathway genes in plants

et al. 2020

View full text Add to dashboard Cite

Background: NADP-malic enzyme (NAPD-ME), and pyruvate orthophosphate dikinase (PPDK) are important enzymes that participate in C 4 photosynthesis. However, the evolutionary history and forces driving evolution of these genes in C 4 plants are not completely understood. Results: We identified 162 NADP-ME and 35 PPDK genes in 25 species and constructed respective phylogenetic trees. We classified NADP-ME genes into four branches, A1, A2, B1 and B2, whereas PPDK was classified into two branches in which monocots were in branch I and dicots were in branch II. Analyses of selective pressure on the NAPD-ME and PPDK gene families identified four positively selected sites, including 94H and 196H in the a5 branch of NADP-ME, and 95A and 559E in the e branch of PPDK at posterior probability thresholds of 95%. The positively selected sites were located in the helix and sheet regions. Quantitative RT-PCR (qRT-PCR) analyses revealed that expression levels of 6 NADP-ME and 2 PPDK genes from foxtail millet were up-regulated after exposure to light. Conclusion: This study revealed that positively selected sites of NADP-ME and PPDK evolution in C 4 plants. It provides information on the classification and positive selection of plant NADP-ME and PPDK genes, and the results should be useful in further research on the evolutionary history of C 4 plants.

show abstract

“…Nevertheless, in most field situations where C 4 crops and grasses form thick canopies, a substantial proportion of the vegetative part of the plant may experience shade or natural low light. Under such unfavourable conditions, C 4 leaves can undergo both biochemical and anatomical changes as part of their acclimatory response (Pengelly et al, ; Sonawane, Sharwood, Whitney, & Ghannoum, ; Tazoe, Hanba, Furumoto, Noguchi, & Terashima, ; Tazoe, Noguchi, & Terashima, ). Responses of C 4 plants, however, appear to differ depending on the decarboxylation subtype (NADP‐ME, NAD‐ME, or PCK; Sonawane et al, ), whether the plants are monocots or dicots (Pengelly et al, ; Sonawane et al, ; Tazoe et al, ; Tazoe et al, ), or even between species (Ward & Woolhouse, ).…”

Section: Introductionmentioning

confidence: 99%

Response of plasmodesmata formation in leaves of C₄ grasses to growth irradiance

Danila

Quick

White

et al. 2019

Plant Cell & Environment

View full text Add to dashboard Cite

Rapid metabolite diffusion across the mesophyll (M) and bundle sheath (BS) cell interface in C 4 leaves is a key requirement for C 4 photosynthesis and occurs via plasmodesmata (PD). Here, we investigated how growth irradiance affects PD density between M and BS cells and between M cells in two C 4 species using our PD quantification method, which combines three-dimensional laser confocal fluorescence microscopy and scanning electron microscopy. The response of leaf anatomy and physiology of NADP-ME species, Setaria viridis and Zea mays to growth under different irradiances, low light (100 μmol m −2 s −1 ), and high light (1,000 μmol m −2 s −1 ), was observed both at seedling and established growth stages. We found that the effect of growth irradiance on C 4 leaf PD density depended on plant age and species.The high light treatment resulted in two to four-fold greater PD density per unit leaf area than at low light, due to greater area of PD clusters and greater PD size in high light plants. These results along with our finding that the effect of light on M-BS PD density was not tightly linked to photosynthetic capacity suggest a complex mechanism underlying the dynamic response of C 4 leaf PD formation to growth irradiance.

show abstract

Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grasses

Abstract: Photosynthesis and productivity are less compromised by low light in NADP-ME C4 grasses relative to PEP-CK and NAD-ME counterparts.

Cited by 48 publications

References 79 publications

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

Identification and evolution of C4 photosynthetic pathway genes in plants

Response of plasmodesmata formation in leaves of C₄ grasses to growth irradiance

Contact Info

Product

Resources

About

Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grasses

Abstract: Photosynthesis and productivity are less compromised by low light in NADP-ME C4 grasses relative to PEP-CK and NAD-ME counterparts.

Cited by 48 publications

References 79 publications

The transcriptomic responses of C4 grasses to subambient CO2 and low light are largely species specific and only refined by photosynthetic subtype

The transcriptomic responses of C4 grasses to subambient CO2 and low light are largely species specific and only refined by photosynthetic subtype

Identification and evolution of C4 photosynthetic pathway genes in plants

Response of plasmodesmata formation in leaves of C4 grasses to growth irradiance

Contact Info

Product

Resources

About

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

The transcriptomic responses of C₄ grasses to subambient CO₂ and low light are largely species specific and only refined by photosynthetic subtype

Response of plasmodesmata formation in leaves of C₄ grasses to growth irradiance