Graphene based composite materials have been extensively studied for the sensing applications attributing to their 2D structures, high conductivity, controlled modification and large specific surface areas, unique mechanical, optical, chemical, electrical, and catalytic properties. Therefore, a number of high quality sensors have been fabricated in recent years. Graphene based composite films (GCFs) that is base of such sensors can be prepared by combining Graphene with different functional nano-materials (carbon materials, noble metals, polymer materials, metal compounds etc.). In this review, we focus on the recent advances in bio-sensing applications of Graphene based composite films.