Shape Engineering Boosts Magnetic Mesoporous Silica Nanoparticle-Based Isolation and Detection of Circulating Tumor Cells

Abstract

Magnetic mesoporous silica nanoparticles (M-MSNs) are attractive candidates for the immunomagnetic isolation and detection of circulating tumor cells (CTCs). Understanding of the interactions between the effects of the shape of M-MSNs and CTCs is crucial to maximize the binding capacity and capture efficiency as well as to facilitate the sensitivity and efficiency of detection. In this work, fluorescent M-MSNs were rationally designed with sphere and rod morphologies while retaining their robust fluorescence and uniform surface functionality. After conjugation with the antibody of epithelial cell adhesion molecule (EpCAM), both of the differently shaped M-MSNs-EpCAM obtained achieved efficient enrichment of CTCs and fluorescent-based detection. Importantly, rodlike M-MSNs exhibited faster immunomagnetic isolation as well as better performance in the isolation and detection of CTCs in spiked cells and real clinical blood samples than those of their spherelike counterparts. Our results showed that shape engineering contributes positively toward immunomagnetic isolation, which might open new avenues to the rational design of magnetic-fluorescent nanoprobes for the sensitive and efficient isolation and detection of CTCs.

Redox/pH dual-controlled release of chlorhexidine and silver ions from biodegradable mesoporous silica nanoparticles against oral biofilms

“…[24][25][26][27] Mesoporous silica nanoparticles (MSNs) have gained substantial attention as promising inorganic nanocarriers for controlled drug delivery because of their large surface area, tunable pore size, and easy surface functionalization. [28][29][30][31][32][33][34][35] In particular, MSNs show the pH-activated release of antibacterial agents to achieve efficient bacterial killing. [36][37][38][39][40] However, the slow degradation of MSNs in biological systems has not yet been fully addressed and remains a major obstacle impeding their further clinical translation.…”

Janus nanocarriers for magnetically targeted and hyperthermia-enhanced curcumin therapy of liver cancer

“…1C, the magnetization saturation value of Janus M-MSNs is 59 emu g À1 , which is lower than pure magnetic sphere whereas higher than core-shell structure according to our previous reports. 35,[40][41][42][43] This is because the coating of silica in core-shell structure decreases the magnetization of magnetic spheres, whereas magnetic spheres in the Janus structure are bare. Hence, the non-interfered structures of Janus M-MSNs led to a better magnetic property in comparison to core-shell M-MSNs.…”

Implantable and Injectable Biomaterial Scaffolds for Cancer Immunotherapy

“…Kim et al developed a promising approach for in situ tumor vaccine based on self-assembled mesoporous silica rods (MSRs) of a high aspect ratio. MSRs have been extensively applied because of the sustained delivery of drugs due to its high porosity, extended superficial area, and biocompatibility (Chang et al, 2018;Wang Z et al, 2018). After administration in mice, this system can nonspecifically assemble into pore structures, allowing for the long and controlled release of payloads.…”