Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells

Stoehr, Linda C.; González, Edgar; Stampfl, Andreas; Casals, Eudald; Duschl, Albert; Puntes, Víctor; Oostingh, Gertie Janneke

doi:10.1186/1743-8977-8-36

Cited by 247 publications

(170 citation statements)

References 39 publications

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“…1 A wide variety of synthesis techniques and routes make it possible to produce Ag nanoparticles (AgNPs) of controlled size, shape, and surface functionality optimized for specific applications. [2][3][4][5][6][7][8][9][10][11][12][13] Pure AgNPs (Ag p NPs) grown and structured in a variety of ways 14 are used in many applications, although challenges related to uniformity and stability remain. 15 There is growing interest in AgNPs with Au cores (Ag Au NPs) due to the ability to tune or optimize their optical and catalytic behaviors.…”

Section: Introductionmentioning

confidence: 99%

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

et al. 2015

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Widespread use of silver nanoparticles raises questions of environmental and biological impact. Many synthesis approaches are used to produce pure silver and silver-shell gold-core particles optimized for specific applications. Since both nanoparticles and silver dissolved from the particles may impact the biological response, it is important to understand the physicochemical characteristics along with the biological impact of nanoparticles produced by different processes. The authors have examined the structure, dissolution, and impact of particle exposure to macrophage cells of two 20 nm silver particles synthesized in different ways, which have different internal structures. The structures were examined by electron microscopy and dissolution measured in Rosewell Park Memorial Institute media with 10% fetal bovine serum. Cytotoxicity and oxidative stress were used to measure biological impact on RAW 264.7 macrophage cells. The particles were polycrystalline, but 20 nm particles grown on gold seed particles had smaller crystallite size with many high-energy grain boundaries and defects, and an apparent higher solubility than 20 nm pure silver particles. Greater oxidative stress and cytotoxicity were observed for 20 nm particles containing the Au core than for 20 nm pure silver particles. A simple dissolution model described the time variation of particle size and dissolved silver for particle loadings larger than 9 μg/ml for the 24-h period characteristic of many in-vitro studies.

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Section: Introductionmentioning

confidence: 99%

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

et al. 2015

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“…9,10 More recently, therefore, silver nanoparticles (AgNPs) have been evaluated as a safer alternative to ionic silver. 1,[11][12][13][14][15][16][17][18][19][20] The recent work from our team showed that in comparison with silver, biomolecule-coated, photochemically-produced AgNPs can have both bactericidal and bacteriostatic properties with almost negligible cytotoxic effects. 12 We also showed that oxidation of Ag to AgO is most likely the cause of the cytotoxic effects observed with AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

et al. 2015

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The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering.The resulting hybrid materials at [AgNPs] < 0.4 µM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 µM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant. IntroductionBiomaterial-associated infections are a significant healthcare problem and have been linked to medical morbidity and death. [2][3][4][5][6] This has motivated the development of materials with anti-infective properties, such as biomaterials loaded with antibiotics. 7 However, with the increasing number of bacteria that are resistant to antibiotics, 8 silver with historically documented anti-microbial activity has re-gained its attractiveness as an alternative to antibiotics. 9 While toxic to bacteria, it unfortunately is also toxic to mammalian cells. 9,10 More recently, therefore, silver nanoparticles (AgNPs) have been evaluated as a safer alternative to ionic silver. 1,[11][12][13][14][15][16][17][18][19][20] The recent work from our team showed that in comparison with silver, biomolecule-coated, photochemically-produced AgNPs can have both bactericidal and bacteriostatic properties with almost negligible cytotoxic effects. 12 We also showed that oxidation of Ag to AgO is most likely the cause of the cytotoxic effects observed with AgNPs. Our overarching goal is to expand the safe use of AgNPs in the development of implantable hybrid-biomaterials with antiinfective properties for future use as scaffolds to enable regeneration of tissue and organs at a risk of bacterial colonization and concomitant biofilm formation like diabetic foot ulcers. Although some collagen-based materials including † Electronic supplementary information (ESI) available: Representative absorption spectra of AgNP@collagen nanoparticles before and after lyophilization. Absorption spectra for the washes obtained from a 1.0 µM AgNP hydrogel over the course of 5 days. Area under the curve (AUC) calculated from the absorption spectra of 500 µm thickness collagen hydrogels prepared using different concentrations of AgNP@collagen. Selected Cryo-SEM images of BDDGE type I collagen-based hydrogels in the absence or presence of 1.0 µM AgNP. An image of a sel...

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“…super-bugs, bacteria strains (Tenover, 2006), has triggered the search for new, more effective, therapies for bacterial infection control, such as nanoparticulated silver (AgNP) (Varner et al, 2010;Rai et al, 2012;Griffith et al, 2015). Despite some studies suggesting that AgNP can present health risks similar to those of ionic silver (Eckhardt et al, 2013;De Alwis Weerasekera et al, 2015), growing evidence indicates otherwise (Asharani et al, 2008;Travan et al, 2009;Moulton et al, 2010;Trickler et al, 2010;Bouwmeester et al, 2011;Stoehr et al, 2011;Alarcon et al, 2012Alarcon et al, , 2013Lu et al, 2012b;Simpson et al, 2013;Vignoni et al, 2014).…”

Section: Nanomaterials As Therapeutic Agents For Skin Regenerationmentioning

confidence: 99%

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

et al. 2016

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Nanomaterials have attracted the interest of tissue engineers for the last two decades. Their unique properties make them promising for de novo fabrication of bio-inspired hybrid/composite materials with improved regenerative properties, including, for example, the capacity for electric conductivity and the provision of antimicrobial properties. However, to this day, the use of such materials in medical applications is rather limited and most of the studies have only reached the archetypical proof-of-concept stage. Herein, we present a review on the use of nanomaterials in tissue engineering for regenerative therapies of heart, skin, eye, skeletal muscle, and nervous system. The advantages and limitations of nano-engineering materials are presented in this review alongside with the future challenges and milestones nanotechnology must overcome to make an impact in biomedical applications.

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Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells

Cited by 247 publications

References 39 publications

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

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