Shark-derived single-domain antibodies for the detection of decapod iridescent virus 1

Chen, Jinlin; Wang, Wei; Zheng, Qin; Cheng, Yunying; Zhang, Qian; Jiang, Sheng; Chen, Jianming

doi:10.1016/j.aqrep.2023.101597

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Moreover, VNAR exhibits the capability to bind to diverse antigens, encompassing proteins, viruses, and small molecules, showcasing significant potential as a diagnostic and therapeutic tool. VNAR can also be extensively engineered into multivalent and specific antigen-binding formats for diagnosis, disease monitoring, and viral infection . Phage display technology employs phages as carriers for presenting foreign proteins or peptides .…”

Section: Introductionmentioning

confidence: 99%

“…22 VNAR surpasses traditional IgG antibodies in terms of high affinity and specificity, nanoscale size, simple structure, remarkable thermal and chemical stability, and cost-effectiveness. 23 Moreover, VNAR exhibits the capability to bind to diverse antigens, encompassing proteins, 24 viruses, 25 and small molecules, 26 showcasing significant potential as a diagnostic and therapeutic tool. VNAR can also be extensively engineered into multivalent and specific antigen-binding formats for diagnosis, disease monitoring, and viral infection.…”

Section: Introductionmentioning

confidence: 99%

“…VNAR can also be extensively engineered into multivalent and specific antigen-binding formats for diagnosis, disease monitoring, and viral infection. 25 Phage display technology employs phages as carriers for presenting foreign proteins or peptides. 27 The advances in phage display technology expand the diversity of peptide recognition molecules for biosensors and hold a crucial role in biodetection.…”

Section: Introductionmentioning

confidence: 99%

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Lateral Flow Immunochromatographic Assay for Competitive Detection of Crustacean Allergen Tropomyosin Using Phage-Displayed Shark Single-Domain Antibody

Jiao,

Xie,

et al. 2024

J. Agric. Food Chem.

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The common food allergy crustacean tropomyosin (TM) poses a significant food safety challenge, which requires rapid and sensitive methods for screening TM in food. Herein, the variable new antigen receptor (VNAR) single-domain antibodies specific for the crustacean TM were isolated from a nai ̈ve phage-displayed shark VNAR library. Subsequently, a lateral flow immunochromatographic assay (LFIA) based on the gold nanoparticle-labeled phage-displayed shark VNAR (AuNPs@PSV) probe was developed for the detection of TM in food. The AuNPs@PSV-LFIA took 15 min for one test and had a visual limit of detection (vLOD) of 0.1 μg/mL and an instrumental LOD of 0.02 μg/mL. Good selectivity, accuracy, precision, and stability were confirmed for the AuNPs@PSV-LFIA. Moreover, the test results of 21 commercially available food products consisted of the allergen labels and were validated by a commercial ELISA kit. Therefore, this work demonstrated the great potential of VNAR for detecting TM in food by LFIA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lateral Flow Immunochromatographic Assay for Competitive Detection of Crustacean Allergen Tropomyosin Using Phage-Displayed Shark Single-Domain Antibody

Jiao,

Xie,

et al. 2024

J. Agric. Food Chem.

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Development of Two Recombinase-Aided Amplification Assays Combined with Lateral Flow Dipstick (Raa-Lfd) and Real-Time Fluorescence (Rf-Raa) for the Detection of Frog Virus 3-Like Ranaviruses

Gui,

Wu,

Liao

et al. 2024

Preprint

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Safety Assessment of Camelid-Derived Single-Domain Antibody as Feed Additive for Juvenile Whiteleg Shrimp (Litopenaeus vannamei) Against White Spot Syndrome Virus

Aulia,

Lim,

Jang

et al. 2024

Animals

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A six-week feeding trial was conducted to assess the safety of single-domain antibodies (sdAbs) derived from camelids against the white spot syndrome virus (WSSV) (WSSVvp28 was used as the antigen), focusing on the whole-organism responses and molecular-level changes in juvenile whiteleg shrimp (Litopenaeus vannamei). Five experimental diets with varying levels of sdAbs were formulated: CON (no sdAb supplementation); SDA8.2 (8.20% of sdAbs); SDA16.4 (16.40% of sdAbs); SDA24.6 (24.60% of sdAbs); and SDA32.8 (32.80% of sdAbs). In the CON diet, 450 mL of water per kg of diet (45%) was used to form a feed dough, while sdAbs were used to replace the water in the treatment diets. A total of 450 shrimp, with an initial body weight of 3.27 ± 0.02 g (mean ± SEM), were randomly distributed in 15 tanks (30 shrimp per tank; three tanks per treatment). Each tank was filled with 30 L of seawater (77 L capacity) in an indoor semi-recirculating system with a constant water flow rate of 1.2 L min−1. The photoperiod was maintained at 12 h of light and 12 h of dark. The water temperature, pH, salinity, and dissolved oxygen were 27.3 ± 0.1 °C, 7.61 ± 0.01, 34 ± 1 ppt, and 5.94 ± 0.04 mg L−1, respectively. During the feeding trial, the shrimp were fed the experimental diet (40% protein and 11% lipid) three times a day for six weeks. Following the feeding trial, an acute cold-water-temperature stress test was conducted by abruptly exposing the shrimp from each treatment to 15 °C for 4 h, down from 27 °C. The results showed no significant differences in the growth performance (weight gain, feed utilization efficiency, survival, etc.), plasma metabolites (aspartate aminotransferase activity, alanine aminotransferase activity, total protein, and glucose), or antioxidant enzymes (superoxide dismutase and glutathione peroxidase) among all the experimental diets (p > 0.05). In the acute cold-temperature stress test, there was no significant interaction between sdAb supplementation and temperature stress, nor any main effect from either factor, except for the main effect of temperature stress on the glucose levels, which was significantly higher in shrimp exposed to cold-temperature stress (p < 0.05). The next-generation sequencing of differentially expressed genes (DEGs) in the hepatopancreases of shrimp fed the CON, SDA16.4, and SDA32.8 diets, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, indicated that DEGs were significantly enriched in signaling pathways associated with growth, cold stress, and antioxidant systems. Overall, the results from conventional measurements suggest that the use of sdAbs against the WSSV may be safe for juvenile whiteleg shrimp. However, findings from the sophisticated analysis indicate that further research is needed to understand the molecular mechanisms underlying the observed changes, and to evaluate the long-term effects of sdAb supplementation in shrimp diets.

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Shark-derived single-domain antibodies for the detection of decapod iridescent virus 1

Cited by 3 publications

References 25 publications

Lateral Flow Immunochromatographic Assay for Competitive Detection of Crustacean Allergen Tropomyosin Using Phage-Displayed Shark Single-Domain Antibody

Lateral Flow Immunochromatographic Assay for Competitive Detection of Crustacean Allergen Tropomyosin Using Phage-Displayed Shark Single-Domain Antibody

Development of Two Recombinase-Aided Amplification Assays Combined with Lateral Flow Dipstick (Raa-Lfd) and Real-Time Fluorescence (Rf-Raa) for the Detection of Frog Virus 3-Like Ranaviruses

Safety Assessment of Camelid-Derived Single-Domain Antibody as Feed Additive for Juvenile Whiteleg Shrimp (Litopenaeus vannamei) Against White Spot Syndrome Virus

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