Shear acoustic wave biosensor for detecting DNA intrinsic viscosity and conformation: A study with QCM-D

Tsortos, Achilleas; Papadakis, George; Gizeli, Electra

doi:10.1016/j.bios.2008.07.006

Cited by 88 publications

(147 citation statements)

References 34 publications

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“…2e, 48-kbp lambda phage DNA in STE buffer was attached to the gold sensor electrode via a complimentary thiolated oligo. Previous studies have shown that, through the use of dissipation monitoring, QCMs are sensitive to not only the adsorbed mass and viscosity, but the physical conformal state ('shape') of DNAs hybridized to the sensor surface 20 . In the experiment, even though the force on the lambda DNAs is on the order of femtonewtons, we observe a strong linear decrease (DG ¼ À 2.9120(95) Hz g À 1 ) in the bandwidth as function of g-force, indicating an increase in viscoelastic loss.…”

Section: δF Loadingmentioning

confidence: 99%

Probing biomechanical properties with a centrifugal force quartz crystal microbalance

2014

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Application of force on biomolecules has been instrumental in understanding biofunctional behaviour from single molecules to complex collections of cells. Current approaches, for example, those based on atomic force microscopy or magnetic or optical tweezers, are powerful but limited in their applicability as integrated biosensors. Here we describe a new force-based biosensing technique based on the quartz crystal microbalance. By applying centrifugal forces to a sample, we show it is possible to repeatedly and non-destructively interrogate its mechanical properties in situ and in real time. We employ this platform for the studies of micron-sized particles, viscoelastic monolayers of DNA and particles tethered to the quartz crystal microbalance surface by DNA. Our results indicate that, for certain types of samples on quartz crystal balances, application of centrifugal force both enhances sensitivity and reveals additional mechanical and viscoelastic properties.

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Section: δF Loadingmentioning

confidence: 99%

Probing biomechanical properties with a centrifugal force quartz crystal microbalance

2014

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“…However, while the system presented here can distinguish between the optical and acoustic masses of the analyte, it has been stated before that the ratio of acoustic dissipation to acoustic frequency contains additional information about the size and shape of a biomolecule [11], [22]. For the current set-up the signal-to-noise ratio for the acoustic dissipation is low, resulting in a significant change in dissipation only for DNA but not for neutravidin (results not shown).…”

Section: Discussionmentioning

confidence: 90%

“…• As a result of the latter, acoustic sensors are sensitive to size and shape of the target molecule [10], [11], [22]. This is important since the conformation and shape of a biomolecule can change as a result of a biochemical reaction or a change in environmental conditions [6], [10], [11].…”

Section: Introductionmentioning

confidence: 99%

Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules

Bender

Roach

Tsortos

et al. 2009

Meas. Sci. Technol.

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It is known that acoustic sensor devices, if operated in liquid phase, are sensitive not just to the mass of the analyte but to various other parameters, such as size, shape, charge and elastic constants of the analyte as well as bound and viscously entrained water. This can be used to extract valuable information about a biomolecule, particularly if the acoustic device is combined with another sensor element which is sensitive to the mass or amount of analyte only. The latter is true in good approximation for various optical sensor techniques. This work reports on the development of a combined surface plasmon resonance / surface acoustic wave sensor system which is designed for the investigation of biomolecules such as proteins or DNA. Results for deposition of neutravidin and DNA are reported.

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“…QCM applications have been extended to measure viscoelastic macromolecular interactions with the surfaces of fluids including natural and synthetic macromolecules such as DNA [5,6], proteins [7,8], microorganisms [9], endothelial cells [10], polymers [11][12][13], and polyelectrolyte multilayers [14]. Through these studies, QCM measurements have shown that viscoelastic fluids containing macromolecules exhibit different behavior from that of Newtonian fluids.…”

Section: Introductionmentioning

confidence: 99%

Effect of a Non-Newtonian Load on SignatureS2for Quartz Crystal Microbalance Measurements

2014

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The quartz crystal microbalance (QCM) is increasingly used for monitoring the interfacial interaction between surfaces and macromolecules such as biomaterials, polymers, and metals. Recent QCM applications deal with several types of liquids with various viscous macromolecule compounds, which behave differently from Newtonian liquids. To properly monitor such interactions, it is crucial to understand the influence of the non-Newtonian fluid on the QCM measurement response. As a quantitative indicator of non-Newtonian behavior, we used the quartz resonator signature, 2 , of the QCM measurement response, which has a consistent value for Newtonian fluids. We then modified De Kee's non-Newtonian three-parameter model to apply it to our prediction of 2 values for non-Newtonian liquids. As a model, we chose polyethylene glycol (PEG400) with the titration of its volume concentration in deionized water. As the volume concentration of PEG400 increased, the 2 value decreased, confirming that the modified De Kee's three-parameter model can predict the change in 2 value. Collectively, the findings presented herein enable the application of the quartz resonator signature, 2 , to verify QCM measurement analysis in relation to a wide range of experimental subjects that may exhibit non-Newtonian behavior, including polymers and biomaterials.

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Shear acoustic wave biosensor for detecting DNA intrinsic viscosity and conformation: A study with QCM-D

Cited by 88 publications

References 34 publications

Probing biomechanical properties with a centrifugal force quartz crystal microbalance

Probing biomechanical properties with a centrifugal force quartz crystal microbalance

Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules

Effect of a Non-Newtonian Load on SignatureS2for Quartz Crystal Microbalance Measurements

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