Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi, Claudia; Pacheco, Daniela; Formenti, Elisa; Pellegata, Alessandro Filippo; Mantero, Sara; Petrini, Paola

doi:10.1016/j.msec.2019.110035

Cited by 10 publications

(8 citation statements)

References 58 publications

(73 reference statements)

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“…Additionally, the loss modulus crossed the storage modulus at very high frequencies, which indicates possible structural rearrangements during the 72 h of the continuous operation in the bioreactor. These results are comparable with those recently reported for a hydrogel-packed bioreactor [ 87 , 88 ].…”

Section: Resultssupporting

confidence: 92%

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Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Patarroyo¹,

Florez-Rojas²,

Pradilla³

et al. 2020

Polymers

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One of the main issues when orally administering microorganism-based probiotics is the significant loss of bioactivity as they pass through the gastrointestinal (GI) tract. To overcome these issues, here, we propose to encapsulate the probiotic yeast Kluyveromyces lactis on chemically crosslinked gelatin hydrogels as a means to protect the bioactive agents in different environments. Hydrogels were prepared by the chemical crosslinking of gelatin, which is commercially available and inexpensive. This is crucial to ensure scalability and cost-effectiveness. To explore changes in key physicochemical parameters and their impact on cell viability, we varied the concentration of the crosslinking agent (glutaraldehyde) and the gelatin. The synthesized hydrogels were characterized in terms of morphological, physical-chemical, mechanical, thermal and rheological properties. This comprehensive characterization allowed us to identify critical parameters to facilitate encapsulation and enhance cell survival. Mainly due to pore size in the range of 5–10 μm, sufficient rigidity (breaking forces of about 1 N), low brittleness and structural stability under swelling and relatively high shear conditions, we selected hydrogels with a high concentration of gelatin (7.5% (w/v)) and concentrations of the crosslinking agent of 3.0% and 5.0% (w/w) for cell encapsulation. Yeasts were encapsulated with an efficiency of about 10% and subsequently tested in bioreactor operation and GI tract simulated media, thereby leading to cell viability levels that approached 95% and 50%, respectively. After testing, the hydrogels’ firmness was only reduced to half of the initial value and maintained resistance to shear even under extreme pH conditions. The mechanisms underlying the observed mechanical response will require further investigation. These encouraging results, added to the superior structural stability after the treatments, indicate that the proposed encapsulates are suitable to overcome most of the major issues of oral administration of probiotics and open the possibility to explore additional biotech applications further.

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Section: Resultssupporting

confidence: 92%

“…bioreactor. These results are comparable with those recently reported for a hydrogel-packed bioreactor [87,88].…”

Section: Figuresupporting

confidence: 92%

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Patarroyo¹,

Florez-Rojas²,

Pradilla³

et al. 2020

Polymers

View full text Add to dashboard Cite

show abstract

“…After the bioreactor operation, the loss modulus crossed the storage modulus at very high frequencies, indicating possible structural rearrangements during the 72 h of the bioreactor's continuous operation. These results are comparable with those recently reported for a hydrogel-packed bioreactor [28,29].…”

Section: Discussionsupporting

confidence: 92%

Formulation and characterization of gelatin-based hydrogels for the encapsulation of <em>Kluyveromyces lactis</em> and chitosan nanoparticles: biotech and biomedical applications

Reyes

Patarroyo

Cruz

2020

Proceedings of 2nd International Online-Conference on Nanomaterials

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A difficulty when orally administering microorganism-based probiotics is the significant loss of their bioactivity as they pass through the gastrointestinal (GI) tract. To overcome these issues, we propose to encapsulate the probiotic yeast Kluyveromyces lactis on chemically crosslinked gelatin hydrogels to protect the bioactive agents in different environments. Moreover, a challenge to obtain favorable results with therapeutic drugs and biomolecules is the inefficient cellular administration process. For this reason, we considered chitosan and gelatin nanoparticle loading systems to improve therapeutic efficacy. Also, we prepared hydrogels to encapsulate such nanoparticles by the chemical crosslinking of gelatin, an inexpensive and commercially available polymer. To explore changes in key physicochemical parameters and their impact on cell viability, we varied the concentration of the crosslinking agent (glutaraldehyde) and the gelatin. The synthesized hydrogels were characterized in morphological, physical-chemical, mechanical, thermal, and rheological properties. This comprehensive characterization allowed us to identify critical parameters to facilitate encapsulation and enhance system performance. Mainly due to pore size in the range of 5-10 µ m, sufficient rigidity (breaking forces of about 1 N), low brittleness and structural stability under swelling and relatively high shear conditions, we selected hydrogels with a high concentration of gelatin (7.5% (w/v)) and concentrations of the crosslinking agent of 3.0% and 5.0% (w/w) for cell and nanoparticles encapsulation. Yeasts and nanoparticles were encapsulated and subsequently tested in bioreactor operation and GI tract simulated media, thereby leading to cell viability levels that approached 95% and 50%, respectively. After testing, the hydrogels' firmness was only reduced to half of the initial value and maintained resistance to shear even under extreme pH conditions. These encouraging results indicate that the proposed encapsulates are suitable for overcoming most of the major issues of oral administration of drugs and probiotics and open the possibility to further explore additional biotech applications.

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“…Based on the responsive stimuli, smart hydrogels could be divided into physical, chemical, and biochemical responsive hydrogels. They are extensively applied in biomedical fields, including therapeutic delivery ( Liang Y. et al, 2019 ), contact lenses ( Alvarez-Rivera et al, 2018 ), corneal prosthesis ( Koivusalo et al, 2019 ), wound healing ( Zhang et al, 2020 ), bone regeneration ( Bao et al, 2020 ), and tissue engineering ( Kaiser et al, 2019 ; Tresoldi et al, 2019 ; George et al, 2020 ; Wang et al, 2020 ).…”

Section: Smart Hydrogel In Biomedical Applicationmentioning

confidence: 99%

Rational Design of Smart Hydrogels for Biomedical Applications

Zhang

Huang

2021

Front. Chem.

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Hydrogels are polymeric three-dimensional network structures with high water content. Due to their superior biocompatibility and low toxicity, hydrogels play a significant role in the biomedical fields. Hydrogels are categorized by the composition from natural polymers to synthetic polymers. To meet the complicated situation in the biomedical applications, suitable host–guest supramolecular interactions are rationally selected. This review will have an introduction of hydrogel classification based on the formulation molecules, and then a discussion over the rational design of the intelligent hydrogel to the environmental stimuli such as temperature, irradiation, pH, and targeted biomolecules. Further, the applications of rationally designed smart hydrogels in the biomedical field will be presented, such as tissue repair, drug delivery, and cancer therapy. Finally, the perspectives and the challenges of smart hydrogels will be outlined.

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Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Cited by 10 publications

References 58 publications

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Formulation and characterization of gelatin-based hydrogels for the encapsulation of <em>Kluyveromyces lactis</em> and chitosan nanoparticles: biotech and biomedical applications

Rational Design of Smart Hydrogels for Biomedical Applications

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