Shear Sensitivity

Chisti, Yusuf

doi:10.1002/9780470054581.eib543

Cited by 20 publications

(15 citation statements)

References 236 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because microalgae are often far more sensitive to turbulence than other microorganisms that are commonly grown in commercial stirred bioreactors. 43 An agitation speed of 300 rpm in a dual impeller bioreactor with a working volume of 2-L of a water-like algal culture, as in the present work, equates to a high level of turbulence. Hydrodynamic forces are known to affect Chlorella vulgaris 44,45 and possibly other members of this genus.…”

Section: Concentration Of Mediummentioning

confidence: 77%

Heterotrophic production of Chlorella sp. TISTR 8990—biomass growth and composition under various production conditions

Bouyam

Choorit

Sirisansaneeyakul

et al. 2017

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

The green microalga Chlorella sp. TISTR 8990 was grown heterotrophically in the dark using various concentrations of a basal glucose medium with a carbon-to-nitrogen mass ratio of 29:1. The final biomass concentration and the rate of growth were highest in the fivefold concentrated basal glucose medium (25 g L glucose, 2.5 g L KNO ) in batch operations. Improving oxygen transfer in the culture by increasing the agitation rate and decreasing the culture volume in 500-mL shake flasks improved growth and glucose utilization. A maximum biomass concentration of nearly 12 g L was obtained within 4 days at 300 rpm, 30°C, with a glucose utilization of nearly 76% in batch culture. The total fatty acid (TFA) content of the biomass and the TFA productivity were 102 mg g and 305 mg L day , respectively. A repeated fed-batch culture with four cycles of feeding with the fivefold concentrated medium in a 3-L bioreactor was evaluated for biomass production. The total culture period was 11 days. A maximum biomass concentration of nearly 26 g L was obtained with a TFA productivity of 223 mg L day . The final biomass contained (w/w) 13.5% lipids, 20.8% protein and 17.2% starch. Of the fatty acids produced, 52% (w/w) were saturated, 41% were monounsaturated and 7% were polyunsaturated (PUFA). A low content of PUFA in TFA feedstock is required for producing high quality biodiesel. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1589-1600, 2017.

show abstract

Section: Concentration Of Mediummentioning

confidence: 77%

Heterotrophic production of Chlorella sp. TISTR 8990—biomass growth and composition under various production conditions

Bouyam

Choorit

Sirisansaneeyakul

et al. 2017

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

show abstract

“…The hydrodynamic shear force imposed through the aeration rate of the reactor system will control the development of biogranules (Chisti, 1999). The size of biogranules is the net result of the balance between the growth and the hydrodynamic shear force imposed by superficial air velocity .…”

Section: Characteristics Of Biogranulesmentioning

confidence: 99%

Sequential Anaerobic-Aerobic Phase Strategy Using Microbial Granular Sludge for Textile Wastewater Treatment

Muda¹,

Aris²,

Salim³

et al. 2013

Biomass Now - Sustainable Growth and Use

View full text Add to dashboard Cite

“…In addition to microfluidic channels, there were other tools, especially viscometers, that had been used for hydrodynamic sensitivity studies. These studies have been reviewed previously (Chalmers, 2000;Chisti, 1999Chisti, , 2000Joshi et al, 1996;Ma et al, 2006;Varley and Birch, 1999).…”

Section: Introductionmentioning

confidence: 99%

Physiological responses of CHO cells to repetitive hydrodynamic stress

Godoy-Silva

Chalmers

Casnocha

et al. 2009

Biotech & Bioengineering

View full text Add to dashboard Cite

A majority of the previous investigations on the hydrodynamic sensitivity of mammalian cells have focused on lethal effects as determined by cell death or lysis. In this study, we investigated the effect of hydrodynamic stress on CHO cells in a fed-batch process using a previously reported system which subjects cells to repetitive, high levels of hydrodynamic stress, quantified by energy dissipation rate (EDR). The results indicated that cell growth and monoclonal antibody production of the test cells were very resistant to the hydrodynamic stress. Compared to the control, no significant variation was observed at the highest EDR tested, 6.4 x 10(6) W/m(3). Most product quality attributes were not affected by intense hydrodynamic stress either. The only significant impact was on glycosylation. A shift of glycosylation pattern was observed at EDR levels at or higher than 6.0 x 10(4) W/m(3), which is two orders of magnitude lower than the EDR where physical cell damage, as measured by lactate dehydrogenase release, was observed. While not as extensively investigated, a second monoclonal antibody produced in a different CHO clone exhibited the same glycosylation change at an intensive EDR, 2.9 x 10(5) W/m(3). Conversely, a low EDR of 0.9 x 10(2) W/m(3) had no effect on the glycosylation pattern. As 6.0 x 10(4) W/m(3), the lowest EDR that triggers the glycosylation shift, is about one order of magnitude higher than the estimated, maximum EDR in typically operated, large-scale stirred tank bioreactors, further studies in a lower EDR range of 1 x 10(3)-6.0 x 10(4) W/m(3) are needed to assess the glycosylation shift effect under typical large-scale bioreactor operation conditions. Follow-up studies in stirred tanks are also needed to confirm the glycosylation shift effect and to validate the repetitive hydrodynamic stress model.

show abstract

Shear Sensitivity

Cited by 20 publications

References 236 publications

Heterotrophic production of Chlorella sp. TISTR 8990—biomass growth and composition under various production conditions

Heterotrophic production of Chlorella sp. TISTR 8990—biomass growth and composition under various production conditions

Sequential Anaerobic-Aerobic Phase Strategy Using Microbial Granular Sludge for Textile Wastewater Treatment

Physiological responses of CHO cells to repetitive hydrodynamic stress

Contact Info

Product

Resources

About