Shell colors and microstructures of four pearl oyster species in the South China Sea

Deng, Zhenghua; Sun, Jing; Wei, Haijun; Wang, Zhao; Chen, Mingqiang; Li, Youning; Yu, Gang; Wang, Yu

doi:10.1016/j.aqrep.2022.101214

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…However, the structure of the periostracal groove is somehow different in that two cell types are distinguishable in C. gigas , columnar cells to the base and planar cells to the tip of the periostracal groove, which are not observed in pearl oysters (Jabbour-Zahab et al, 1992;Parvizi et al, 2017). Given the morphological similarities in the mantle tissue between C. gigas and pearl oysters, it is likely that the differences observed, in terms of the shape and microstructure of their shells (Farre et al, 2011;Lee et al, 2011;Marie et al, 2011;Marie et al, 2012;Mouchi et al, 2016;Checa et al, 2018;Deng et al, 2022), are the product of different shell matrix proteins secreted by the mantle in a species-specific manner (Aguilera et al, 2017), rather than as a product of mantle morphology. Inside the mantle tissue, there are contractile muscle fibers possibly involved in mantle retraction and adipose tissue containing high glycogen content (Berthelin et al, 2000;Álvarez Nogal and Molist García, 2015;Parvizi et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

Zúñiga-Soto

Pinto-Borguero

Quevedo

et al. 2023

Preprint

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Since the Industrial Revolution, the concentration of atmospheric carbon dioxide (CO2) due to anthropogenic activities has increased at unprecedented rates. One-third of the atmospheric anthropogenic CO2 emissions are dissolved in the oceans affecting the chemical equilibrium of seawater, which in turn leads to a decrease in pH and carbonate ion (CO32-) concentration, a phenomenon known as ocean acidification (OA). This chemical disequilibrium can be detrimental to marine organisms (e.g., mollusks) that fabricate mineralized structures based on calcium carbonate (CaCO3). Most studies on the effect of reduced pH in seawater have been conducted on the early developmental stages of shell-building invertebrates, neglecting how adult individuals face OA stress. Here, we evaluate histological, secretory, and transcriptional changes in the mantle of adult oysters (Crassostrea gigas) exposure to ambient (8.0 +- 0.2) and reduced (7.6 +- 0.2) pH during 20 days. Most histological observations did not show differences in terms of mantle cell morphology. However, Alcian Blue/PAS staining revealed significant differences in the number of Alcian Blue positive cells in the mantle edge, suggesting a decrease in the secretory activity in this morphogenetic zone. Transcriptomic analysis revealed 172 differentially expressed genes (DEGs) between mantle tissues from adult oysters kept in normal and reduced pH conditions. Almost 18% of the DEGs encode secreted proteins that are likely to be contributing to shell fabrication and patterning. 17 of 31 DEGs encoding secreted proteins correspond to oyster-specific genes, highlighting the fact that molluscan shell formation is underpinned by a rapidly evolving secretome. The GO analysis of DEGs encoding secreted proteins showed that they are involved in the cellular response to stimulus, response to stress, protein binding, and ion binding, suggesting these biological processes and molecular functions are altered by OA. This study demonstrates that histology and gene expression profiling can advance our understanding of the cellular and molecular mechanisms underlying adult oyster tolerance to low pH conditions.

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Section: Discussionmentioning

confidence: 99%

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

Zúñiga-Soto

Pinto-Borguero

Quevedo

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the structure of the periostracal groove is somehow different in that two cell types are distinguishable in C. gigas, columnar cells to the base and planar cells to the tip of the periostracal groove, which are not observed in pearl oysters (Jabbour-Zahab et al, 1992;Parvizi et al, 2017). Given the morphological similarities in the mantle tissue between C. gigas and pearl oysters, it is likely that the differences observed, in terms of the shape and microstructure of their shells (Farre et al, 2011;Lee et al, 2011;Marie et al, 2011;Marie et al, 2012;Mouchi et al, 2016;Checa et al, 2018;Deng et al, 2022), are the product of different shell matrix proteins secreted by the mantle in a species-specific manner (Aguilera et al, 2017), rather than as a product of mantle morphology. Inside the mantle tissue, there are contractile muscle fibers possibly involved in mantle retraction and adipose tissue containing high glycogen content (Berthelin et al, 2000;A ́lvarez Nogal and Molist Garcıá, 2015;Parvizi et al, 2017).…”

Section: Effects Of Ocean Acidification On Mantle Anatomy and Cell Mo...mentioning

confidence: 99%

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

et al. 2023

View full text Add to dashboard Cite

Since the Industrial Revolution, the concentration of atmospheric carbon dioxide (CO2) due to anthropogenic activities has increased at unprecedented rates. One-third of the atmospheric anthropogenic CO2 emissions are dissolved in the oceans affecting the chemical equilibrium of seawater, which in turn leads to a decrease in pH and carbonate ion (CO32-) concentration, a phenomenon known as ocean acidification (OA). This chemical disequilibrium can be detrimental to marine organisms (e.g., mollusks) that fabricate mineralized structures based on calcium carbonate (CaCO3). Most studies on the effect of reduced pH in seawater have been conducted on the early developmental stages of shell-building invertebrates, given less attention to how adult individuals face OA stress. Here, we evaluate histological, secretory, and transcriptional changes in the mantle of adult oysters (Crassostrea gigas) exposure to ambient (8.0 ± 0.2) and reduced (7.6 ± 0.2) pH during 20 days. Most histological observations did not show differences in terms of mantle cell morphology. However, Alcian Blue/PAS staining revealed significant differences in the number of Alcian Blue positive cells in the mantle edge, suggesting a decrease in the secretory activity in this morphogenetic zone. Transcriptomic analysis revealed 172 differentially expressed genes (DEGs) between mantle tissues from adult oysters kept in normal and reduced pH conditions. Almost 18% of the DEGs encode secreted proteins that are likely to be contributing to shell fabrication and patterning. 17 of 31 DEGs encoding secreted proteins correspond to oyster-specific genes, highlighting the fact that molluscan shell formation is underpinned by a rapidly evolving secretome. The GO analysis of DEGs encoding secreted proteins showed that they are involved in the cellular response to stimulus, response to stress, protein binding, and ion binding, suggesting these biological processes and molecular functions are altered by OA. This study demonstrates that histology and gene expression profiling can advance our understanding of the cellular and molecular mechanisms underlying adult oyster tolerance to low pH conditions.

show abstract

Study on the Development of an Oyster Shell-Based Clarifier for Enhancing Sugar Industry Processes

Hu,

Mo,

Zhang

2024

Sugar Tech

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Shell colors and microstructures of four pearl oyster species in the South China Sea

Cited by 5 publications

References 41 publications

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

Study on the Development of an Oyster Shell-Based Clarifier for Enhancing Sugar Industry Processes

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