Shell-crosslinked Pluronic L121 micelles as a drug delivery vehicle

Yang, Ting; Chen, Chun Nan; Chen, Mei-Chin; Lai, Chien Hsun; Liang, Hsiang Fa; Sung, Hsing‐Wen

doi:10.1016/j.biomaterials.2006.09.035

Cited by 127 publications

(84 citation statements)

References 30 publications

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“…Then they were placed into the wells and the cells were allowed to attach to the surfaces of hydrogels for 7 days. On Days 1, 2, 4, and 7, the viability of cells was quantitatively measured by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, 34 the morphology of the cells, which were stained with crystal violet, was observed using an inverted phase contrast microscope (Leica). All experiments were performed in triplicate at least.…”

Section: Cell Culturementioning

confidence: 99%

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Zhang

Wang

Song

et al. 2011

J of Applied Polymer Sci

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This work describes a comprehensive study of hydrogels based on polyethylene glycol diacrylates (PEGDAs) with the molecular weight (MW) range of 400-2000. The blends of low-and high-molecular weight PEGDA macromers with different ratios were photopolymerized under visible light irradiation, using a blue light sensitive photoinitiator Irgacure819, at the total polymer concentration of 60 wt %. Swelling ratios, wetting property, elastic moduli, transparency, and the microstructure of the resulting hydrogels were investigated. Among them, equilibrium water contents, hydrophilicity, and mesh size of the hydrogels increased while the elastic moduli decreased when increased the PEGDA MW or the content of higher MW PEGDA in the blends. Most of the hydrogels possessed excellent transparency in visible region. The viability of L929 cells on the surface of hydrogel was also estimated. All the selected hydrogels exhibited a relatively high proliferation rate, which demonstrated this hydrogel system with photoinitiator Irgacure819 had good biocompatibility. These results show the properties of PEGDA hydrogel could be easily adjusted by varying PEGDA MW or the ratios of low-and high-MW macromers in the composites. It could be helpful for the design of proper PEGDA hydrogels in the applications as tissue engineering or drug delivery system.

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Section: Cell Culturementioning

confidence: 99%

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Zhang

Wang

Song

et al. 2011

J of Applied Polymer Sci

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“…The cell uptake was evaluated by fluorescence microscopy (Yang et al, 2007). Rh-123 (a poorly water-soluble fluorescent substance) was loaded into the micelles using the same method with preparation of PTX-loaded micelles.…”

Section: In Vitro Cytotoxicity Assaymentioning

confidence: 99%

Preparation of sodium cholate-based micelles through non-covalent ıbonding interaction and application as oral delivery systems for paclitaxel

Zhao

2015

Drug Delivery

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In present study, two types of micelles based on sodium cholate (NaC) were prepared through non-covalent bonding interaction and the potential of micelles as oral drug delivery systems for paclitaxel (PTX) was evaluated. Pluronic-chitosan (F127-CS) and Pluronic-poly (acrylic acid) (F127-PAA) copolymers were synthesized. Electrostatic interaction and hydrogen bond were used to prepare F127-CS/NaC micelles and F127-PAA/NaC micelles, respectively. The physicochemical characteristics of micelles were determined. An average diameter of 67.5 nm and unimodal pattern of size distribution were observed for F127-CS/NaC micelles. While for F127-PAA/NaC micelles, an average diameter of 85.89 nm and non-unimodal pattern of size distribution were observed. The results revealed that F127-CS/NaC micelles were more integrated than F127-PAA/NaC micelles. Further experiments showed that the F127-CS/NaC micelles had a higher drug-loading content of 12.8% and a lower critical micelle concentration (CMC) of 2.5 Â 10 À3 mol/L compared with F127-PAA/NaC micelles. In vitro cytotoxicity analysis demonstrated that the PTX-loaded F127-CS/NaC micelles were of great efficiency in inhibiting the growth of drug-resistant breast cancer MCF-7 cells (MCF-7/Adr). The intragastric administration of the PTX-loaded F127-CS/NaC micelles in rats provided a 4.33-fold higher absolute bioavailability compared to commercial Taxol Õ , indicating an efficient oral absorption of PTX delivered by micelles. These findings signify that F127-CS/NaC micelle may be a promising carrier for the delivery of PTX.

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“…However, DOX-P micelles showed tightly packed nanospheres with an average size of about 20 nm ( Figure 5B). It was likely that hydrophobic interaction stacking of conjugated DOX molecules in the core were responsible for the self-assembling core/shell structure of DOX-P micelles, which would make the micelles more stable in theory [12]. It is known that drug encapsulation in micelles can diminish drug extravasation into normal tissues and provide for a passive drug targeting to tumors via the enhanced permeability and retention (EPR) effect.…”

Section: Tem Of Dox-p Micellesmentioning

confidence: 99%

“…Dilution in the blood upon injection would quickly dissolve the micelle and prematurely release the drug into the blood stream, which limits their practical uses for systemic delivery of DOX. To enhance stability of micelles in the blood stream upon dilution, Pluronic L121 micelles were cross-linked through their hydrophilic shells [12]. To form the crosslinks, the end hydroxyl groups of Pluronic L121 were first chemically converted to aldehydes and then bridged via Schiff bases.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Doxorubicin-Pluronic F68 Conjugate Micelles and Their Effect on Doxorubicin Resistant Human Erythroleukemic Cancer Cells

Sun¹,

Lu²,

Zhao³

2011

J Nanomedic Nanotechnol

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Doxorubicin-pluronic F68 conjugate (DOX-P) was synthesized and its structure was confirmed by FTIR and 1 H-NMR spectra. Using human erythroleukemic cancer cells as model, DOX-P application in chemotherapy was further investigated. Differential scanning calorimetry analysis was applied to compare the fusion and crystallization characterization between pluronic F68 and DOX-P. Morphology and size assessment were measured using a transmission electron microscopy (TEM) to confirm the capability of forming micelles of DOX-P. Tumor cell lines K562 and K562/AO2 were used to investigate the effect of DOX-P on tumor cell resistance. The Tm and Tc of DOX-P were lower than pluronic F68 resulted from the connection of DOX to pluronic F68. Morphology images confirmed the existence of DOX-P micelles, with an average size of about 20 nm. Drug release profile showed that the DOX-P conjugate maintained a sustained DOX release. From cell experiment in vitro, DOX-P micelles could circumvent the DOX resistance of K562/AO2 cells. With advantages of EPR effect and reducing tumor resistance, DOX-P micelles might develop as new tumor targeted delivery system for chemotherapy. HO

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Shell-crosslinked Pluronic L121 micelles as a drug delivery vehicle

Cited by 127 publications

References 30 publications

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Controllable properties and microstructure of hydrogels based on crosslinked poly(ethylene glycol) diacrylates with different molecular weights

Preparation of sodium cholate-based micelles through non-covalent ıbonding interaction and application as oral delivery systems for paclitaxel

Characterization of the Doxorubicin-Pluronic F68 Conjugate Micelles and Their Effect on Doxorubicin Resistant Human Erythroleukemic Cancer Cells

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