Shewanella species as the origin of blaOXA-48 genes: insights into gene diversity, associated phenotypes and possible transfer mechanisms

Tacão, Marta; Araújo, Susana; Vendas, Maria do Carmo Oliveira; Alves, Artur; Henriques, Isabel

doi:10.1016/j.ijantimicag.2017.05.014

Cited by 42 publications

(37 citation statements)

References 38 publications

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“…Further, the Shewanella sp. BC20 genome harbors a class D β-lactamase, bla OXA-48 , with the same upstream and downstream genetic context that others have reported (Tacao et al, 2018). Numerous reports suggest the occurrence of bla OXA-48 in environmental Shewanella spp.…”

Section: Discussionmentioning

confidence: 75%

“…BC20, isolated from a heavy-metal contaminated estuary, showed resistance to carbapenems, cephalosporins, and penicillins; three classes of drugs to which Shewanella are commonly susceptible (Janda, 2014). The strain showed an elevated resistance profile compared to the 28 out of the 33 environmental Shewanella isolates tested by Tacao et al (Tacao et al, 2018), with resistance to 9 of the antibiotics reported. Further, the Shewanella sp.…”

Section: Discussionmentioning

confidence: 99%

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Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Lloyd

Nazaret

Barkay

2018

Marine Pollution Bulletin

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Antibiotic resistance is a global public health issue and metal exposure can co-select for antibiotic resistance. We examined genome sequences of three multi-drug and metal resistant bacteria: one Shewanella sp., and two Vibrio spp., isolated from the gut of the mummichog fish (Fundulus heteroclitus). Our primary goal was to understand the mechanisms of co-selection. Phenotypically, the strains showed elevated resistance to arsenate, mercury, and various types of β-lactams. The genomes contained genes of public health concern including one carbapenemase (bla OXA-48). Our analyses indicate that the co-selection phenotype is mediated by chromosomal resistance genes and cross-resistance. No evidence of co-resistance was found; most resistance genes were chromosomally located. Moreover, the identification of many efflux pump gene homologs indicates that cross-resistance and/or co-regulation may further contribute to resistance. We suggest that the mummichog gut microbiota may be a source of clinically relevant antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Lloyd

Nazaret

Barkay

2018

Marine Pollution Bulletin

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“…The KPC gene has been transferred to many different plasmids, is now widely dispersed amongst Enterobacteriaceae and has also found its way into Pseudomonas [28] and Acinetobacter [29]. The OXA--48 carbapenemase originates from Shewanella [30] and was first detected in K. pneumoniae in Turkey in 2003 [31]. It was initially associated with hospital outbreaks across Europe and is now reported worldwide [32], although not as widely dispersed as KPC.…”

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confidence: 99%

Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria

Wyres

Holt

2018

Preprint

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Klebsiella pneumoniae is an opportunistic bacterial pathogen known for its high frequency and diversity of antimicrobial resistance (AMR) genes. In addition to being a significant clinical problem in its own right, K. pneumoniae is the species within which several new AMR genes were first discovered before spreading to other pathogens (e.g. carbapenem--resistance genes KPC, OXA--48 and NDM--1). Whilst K. pneumoniae's contribution to the overall AMR crisis is impossible to quantify, current evidence suggests it has a wider ecological distribution, significantly more varied DNA composition, greater AMR gene diversity and a higher plasmid burden than other Gram negative opportunists. Hence we propose it plays a key role in disseminating AMR genes from environmental microbes to clinically important pathogens.

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“…Accordingly, we did not identify any insertion element (IS) upstream of the bla OXA-535 gene that could provide strong promoter sequences, unlike the plasmidencoded OXA-181 and OXA-48, where ISEcp1 and IS1999, respectively, allow strong carbapenemase expression. Like in other sequenced Shewanella spp., we identify a gene coding for a peptidase C15 family upstream of bla OXA-535 and a lysR gene coding for a putative LysR transcriptional regulator downstream (13).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, analysis of publicly available Shewanella species genomes revealed the presence of several uncharacterized bla OXA-48 like genes, confirming the importance of Shewanella spp. as a OXA-48-like class D ␤-lactamase reservoir (13,14).…”

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confidence: 99%

CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436

Jousset

Dabos

Bonnin

et al. 2018

Antimicrob Agents Chemother

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spp. constitute a reservoir of antibiotic resistance determinants. In a bile sample, we identified three extended-spectrum-β-lactamase (ESBL)-producing bacteria (, , and sp. strain JAB-1) isolated from a child suffering from cholangitis. Our objectives were to characterize the genome and the resistome of the first ESBL-producing isolate of the genus and determine whether plasmidic exchange occurred between the three bacterial species. Bacterial isolates were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), standard biochemical tools, and antimicrobial susceptibility testing. sp. JAB-1 and ESBL gene-encoding plasmids were characterized using PacBio and Illumina whole-genome sequencing, respectively. The sp. JAB-1 chromosome-encoded OXA-48 variant was cloned and functionally characterized. Whole-genome sequencing (WGS) of the sp. clinical isolate JAB-1 revealed the presence of a 193-kb plasmid belonging to the IncA/C incompatibility group and harboring two ESBL genes, and gene-carrying plasmids belonging to the IncY and IncR incompatibility groups were also found in the and isolates from the same patient, respectively. A comparison of the genetic environment indicated the independent origin of these plasmids and dismissed transfers. Furthermore, characterization of the resistome of sp. JAB-1 revealed the presence of a chromosome-carried gene, likely the progenitor of the plasmid-carried gene, a novel-like gene. The expression of in showed the carbapenem-hydrolyzing activity of OXA-535. The production of OXA-535 in sp. JAB-1 could be evidenced using molecular and immunoenzymatic tests, but not with biochemical tests that monitor carbapenem hydrolysis. In this study, we have identified a CTX-M-15-producing species that was responsible for a hepatobiliary infection and that is likely the progenitor of OXA-436, a novel plasmid-encoded OXA-48-like class D carbapenemase.

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Shewanella species as the origin of blaOXA-48 genes: insights into gene diversity, associated phenotypes and possible transfer mechanisms

Cited by 42 publications

References 38 publications

Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria

CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436

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