Shifts in bacterial communities and antibiotic resistance genes in surface water and gut microbiota of guppies (Poecilia reticulata) in the upper Rio Uberabinha, Brazil

Jia, Jia; Silva, Guilherme Gomes; Plath, Martin; Pereira, Boscolli Barbosa; Ueira-Vieira, Carlos; Wang, Zaizhao

doi:10.1016/j.ecoenv.2021.111955

Cited by 20 publications

(14 citation statements)

References 45 publications

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“…Additionally, the related bacterial classes Mollicutes and Clostridia were observed to be in higher abundance in OTC-treated fish. These results corroborate a previous study where the abundance of Tenericutes was higher in Poecilia reticulata gut growing in water bodies contaminated with antibiotic discharges from the nearby industries [ 26 ]. Tenericutes and its associated class, Mollicutes, are distinct taxonomic units that lack a cell wall that makes easier it for antibiotic resistance genes (ARGs) to be transformed into the bacterial cells [ 27 ].…”

Section: Discussionsupporting

confidence: 92%

“…Such observations can be attributed to decrease in cellular processes of gut microbiome of fish upon exposure to OTC [ 31 ], leading to decrease in ribosomal biogenesis and cell division. On the other hand, the increase in membrane efflux system and amino acid metabolism are atypical for gut bacterial diversity of fish upon antibiotic treatments [ 26 , 32 ]. The increase in abundance of membrane efflux systems can assist in expelling OTC from the intra-cellular milieu of bacteria and correlate to their survival in antibiotic rich environmental niche [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

Choudhury

Park

Kim

et al. 2021

Animals

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The extensive use of antibiotics is evident in most of the livestock and aquaculture management for inhibiting pathogen infection. Korean aquaculture depends on the usage of oxy-tetracycline for growing rainbow trout. Hence, this study was conducted to evaluate the changes in gut bacterial community profiles of rainbow trout exposed to oxy-tetracycline and predict the metabolic functioning of the bacterial community. The gut bacterial community composition of oxy-tetracycline treated fish was assessed by amplicon sequencing targeting the 16S rRNA gene of bacteria and comparing with the control group that did not receive any antibiotic. The principle coordinate analysis and non-metric multidimensional scaling analysis had shown two distinct clusters that implies the changes in community composition. In phyla level, the relative abundances of Tenericutes and Firmicutes were observed to be significantly higher in oxy-tetracycline treated fish compared to the control. Furthermore, the prediction based metabolic profiling revealed the processes that are affected due to the shift in community profiles. For example, metabolic functioning of membrane efflux system, amino acid metabolism and glycolysis were significantly higher in oxy-tetracycline treated fish compared to the control. This study describes alteration in gut bacterial community composition and potential metabolic profiles of the community that might be responsible for surviving in antibiotic rich environment.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

Choudhury

Park

Kim

et al. 2021

Animals

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“…Despite the concerns related to the spread of antibiotic resistance (AR) directly from polluted water, this is not the only way for AR dissemination. Antimicrobial resistance genes (ARG) and antimicrobial resistant bacteria (ARB) can reach the environment from wild animal microbiomes and from the selective pressure of metals in the environment by a co-resistance phenomenon, which have been recently reported for superficial Brazilian water [45,46]. As a natural phenomenon, it can be inferred that antibiotic resistance spread in nature can be intrinsically controlled.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Pharmaceuticals and Endocrine Disrupting Compounds in Brazilian Water and the Risks They May Represent to Human Health

Aquino

Brandt

Bottrel

et al. 2021

IJERPH

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The risks of pharmaceuticals and endocrine disrupting compounds (P&EDC) to the environment and human health are a current topic of interest. Hundreds of P&EDC may reach the environment, hence, there is a need to rank the level of concern of human exposure to these compounds. Thus, this work aimed at setting a priority list of P&EDC in Brazil, by studying their occurrence in raw and drinking water, calculating health guideline values (GV), and estimating the risks of population exposure to water intake. Data on the Brazilian pharmaceutical market as well as published data of the monitoring of Brazilian natural and drinking water have been collected by means of an exhaustive literature review. Furthermore, many foreign data were also collected to enable a comparison of the values found in Brazilian studies. A list of 55 P&EDC that have the potential to be found in Brazilian water is proposed, and for 41 of these a risk assessment was performed by estimating their margin of exposure (ME), by considering their occurrence in drinking water, and guideline values estimated from reported acceptable daily intake (ADI) data. For seven compounds the risk was deemed high (three estrogens and four anti-inflammatories), whereas for another seven compounds, it was regarded as an ‘alert’ situation. Although such risk analysis is conservative, since it has been calculated based on the highest reported P&EDC concentration in drinking water, it highlights the need to enhance their monitoring in Brazil to strengthen the database and support decision makers. An analysis of the occurrence of antimicrobial resistance agents (antibiotics, resistant bacteria, and resistance genes) in surface waters was also carried out and confirmed that such agents are present in water sources throughout Brazil, which deserves the attention of policy makers and health agents to prevent dissemination of antimicrobial resistance through water use.

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“…While ammonia was detected in both DWW (0.75) and HWW (0.13), nutrients such as nitrites and nitrates were not detected in HWW. Literature evidence suggests that the presence of antibiotic compounds in the environment is detrimental, as they may kill off nitrifying and denitrifying bacteria, impacting the process of nitrogen fixation in the terrestrial environment [ 33 ] while at the same time exerting a selective pressure that favors the proliferation of antibiotic-resistant bacterial communities [ 34 , 35 ]. The concentration of chloride and fluoride ions in HWW exceeded the EPA permissible limits as well as the Indian standards for wastewater discharge.…”

Section: Discussionmentioning

confidence: 99%

Taxonomic and Functional Distribution of Bacterial Communities in Domestic and Hospital Wastewater System: Implications for Public and Environmental Health

Selvarajan

Sibanda

Pandian³

et al. 2021

Antibiotics

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The discharge of untreated hospital and domestic wastewater into receiving water bodies is still a prevalent practice in developing countries. Unfortunately, because of an ever-increasing population of people who are perennially under medication, these wastewaters contain residues of antibiotics and other antimicrobials as well as microbial shedding, the direct and indirect effects of which include the dissemination of antibiotic resistance genes and an increase in the evolution of antibiotic-resistant bacteria that pose a threat to public and environmental health. This study assessed the taxonomic and functional profiles of bacterial communities, as well as the antibiotic concentrations in untreated domestic wastewater (DWW) and hospital wastewater (HWW), using high-throughput sequencing analysis and solid-phase extraction coupled to Ultra-high-performance liquid chromatography Mass Spectrometry (UHPLC–MS/MS) analysis, respectively. The physicochemical qualities of both wastewater systems were also determined. The mean concentration of antibiotics and the concentrations of Cl−, F− and PO43 were higher in HWW samples than in DWW samples. The phylum Firmicutes was dominant in DWW with a sequence coverage of 59.61% while Proteobacteria was dominant in HWW samples with a sequence coverage of 86.32%. At genus level, the genus Exiguobacterium (20.65%) and Roseomonas (67.41%) were predominant in DWW and HWW samples, respectively. Several pathogenic or opportunistic bacterial genera were detected in HWW (Enterococcus, Pseudomonas and Vibrio) and DWW (Clostridium, Klebsiella, Corynebacterium, Bordetella, Staphylocccus and Rhodococcus) samples. Functional prediction analysis indicated the presence of beta-lactam resistance, cationic antimicrobial peptide (CAMP) resistance and vancomycin resistance genes in HWW samples. The presence of these antibiotic resistance genes and cassettes were positively correlated with the presence of pathogens. These findings show the risk posed to public and environmental health by the discharge of untreated domestic and hospital wastewaters into environmental water bodies.

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Shifts in bacterial communities and antibiotic resistance genes in surface water and gut microbiota of guppies (Poecilia reticulata) in the upper Rio Uberabinha, Brazil

Cited by 20 publications

References 45 publications

Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

Occurrence of Pharmaceuticals and Endocrine Disrupting Compounds in Brazilian Water and the Risks They May Represent to Human Health

Taxonomic and Functional Distribution of Bacterial Communities in Domestic and Hospital Wastewater System: Implications for Public and Environmental Health

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