Shifts of Bacterioplankton Metabolic Profiles along the Salinity Gradient in a Subtropical Estuary

Urakawa, Hidetoshi; Ali, Jaffar; Ketover, Rheannon D. J.; Talmage, Spencer D.; García, Juan Carlos; Campbell, Ian S.; Loh, Andrew; Parsons, M. L.

doi:10.5402/2013/410814

Cited by 12 publications

(7 citation statements)

References 33 publications

(52 reference statements)

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“…The overall CLPPs showed that the metabolic diversity was significantly enhanced from the summer (when ambient and water temperatures were the highest) to fall season, but it dropped rapidly in the winter (when ambient and water temperatures were the lowest). Similar results were found by Urakawa et al [ 49 ] who reported that bacterioplankton assemblages in the summer and fall showed more versatile substrate utilization patterns than winter and spring communities.…”

Section: Discussionsupporting

confidence: 90%

Patterns of Change in Metabolic Capabilities of Sediment Microbial Communities in River and Lake Ecosystems

Oest

Alsaffar

Fenner

et al. 2018

International Journal of Microbiology

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Information on the biodegradation potential of lake and river microbial communities is essential for watershed management. The water draining into the lake ecosystems often carries a significant amount of suspended sediments, which are transported by rivers and streams from the local drainage basin. The organic carbon processing in the sediments is executed by heterotrophic microbial communities, whose activities may vary spatially and temporally. Thus, to capture and apprehend some of these variabilities in the sediments, we sampled six sites: three from the Saint Clair River (SC1, SC2, and SC3) and three from Lake Saint Clair in the spring, summer, fall, and winter of 2016. Here, we investigated the shifts in metabolic profiles of sediment microbial communities, along Saint Clair River and Lake Saint Clair using Biolog EcoPlates, which test for the oxidation of 31 carbon sources. The number of utilized substrates was generally higher in the river sediments (upstream) than in the lake sediments (downstream), suggesting a shift in metabolic activities among microbial assemblages. Seasonal and site-specific differences were also found in the numbers of utilized substrates, which were similar in the summer and fall, and spring and winter. The sediment microbial communities in the summer and fall showed more versatile substrate utilization patterns than spring and winter communities. The functional fingerprint analyses clearly distinguish the sediment microbial communities from the lake sites (downstream more polluted sites), which showed a potential capacity to use more complex carbon substrates such as polymers. This study establishes a close linkage between physical and chemical properties (temperature and organic matter content) of lake and river sediments and associated microbial functional activities.

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Section: Discussionsupporting

confidence: 90%

Patterns of Change in Metabolic Capabilities of Sediment Microbial Communities in River and Lake Ecosystems

Oest

Alsaffar

Fenner

et al. 2018

International Journal of Microbiology

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“…More specifically, after grouping every carbon source by chemical category, amino acids, carbohydrates and phenolic substances were highly consumed by BCs associated with N 2 - cyanobacteria ( Figure 3A ). Similarly, the NUI ( Urakawa et al, 2013 ) was significantly higher for BCs associated with N 2 - cyanobacteria than for those associated with N 2 + cyanobacteria (ANOVA analysis: df = 1, F -value = 289.5, p = 9.3 10 -14 ) ( Figure 3B ). Overall, among the different characteristics of the cyanobacterial strains (N 2 fixation, culture medium, date of isolation, genus and putative toxicity), only N 2 fixation/culture medium and the interactions among cyanobacterial strain, genus and N 2 fixation/culture medium had significant effects on carbon and nitrogen metabolic activities [ANOVA analyses using best models given by AIC analyses (∼Fixation + Fixation:Strain:Genus), df = 1 and F -value > 126.1 for N 2 fixation; df = 13 and F -value < 18.1 for Fixation:Strain:Genus, p < 0.001].…”

Section: Resultsmentioning

confidence: 91%

Bacterial Communities Associated with Four Cyanobacterial Genera Display Structural and Functional Differences: Evidence from an Experimental Approach

et al. 2016

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To overcome the limitations associated with studying the interactions between bacterial communities (BCs) and cyanobacteria in natural environments, we compared the structural and functional diversities of the BCs associated with 15 non-axenic cyanobacterial strains in culture and two natural BCs sampled during cyanobacterial blooms. No significant differences in richness and diversity were found between the natural and cultivated BCs, although some of the cyanobacterial strains had been isolated 11 years earlier. Moreover, these BCs shared some similar characteristics, such as a very low abundance of Actinobacteria, but they display significant differences at the operational taxonomic unit (OTU) level. Overall, our findings suggest that BCs associated with cyanobacteria in culture are good models to better understand the interactions between heterotrophic bacteria and cyanobacteria. Additionally, BCs associated with heterocystous cyanobacterial strains cultivated in Z8X culture medium without nitrate (Aphanizomenon–Dolichospermum) demonstrated significant differences compared to BCs associated with non-heterocystous strains cultivated in Z8 culture medium (Planktothrix–Microcystis) in terms of their composition and their ability to utilize different carbon sources, suggesting the potential influence of cyanobacterial metabolism and/or culture media on associated BCs. Finally, half of the dominant OTUs in these BCs were specifically associated with cyanobacteria or other phytoplankton, whereas the remaining OTUs were generally associated with ecosystems containing high organic matter content, such as sludge or intestines.

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“…4b). Urakawa et al (2013) demonstrated that the detected variability among the environmental samples was not due to technical errors or poor reproducibility of CLPP methods, but rather by the community-level heterogeneity in the analyzed microbial samples. Thus, our observation indicates that toxic substances (such as pharmaceuticals) might be present in WWTP e uents, and these may have inhibited bacterial diversity at WWD sites compared to other sites in the fall (Nega et al 2019).…”

Section: Spatial and Seasonal Variations In Microbial Biomassmentioning

confidence: 94%

Spatio-temporal variations in the physiological profiles of streambed bacterial communities: implication of wastewater treatment plant effluents

Akinwole

Guta

Draper

et al. 2021

World J Microbiol Biotechnol

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The e uents of wastewater treatment plants (WWTPs) represent a complex mixture of nutrients and toxic substances, thus, the potential exists for the e uents to signi cantly impact the biochemical characteristics and bacterial communities of the receiving water. We examined spatial and seasonal patterns, and the impact of e uents on microbial biomass, bacterial community structure, and metabolic diversity on a fourth-order stream. We took triplicate sediment samples at ve different locations along a 5,000m transect over three sampling periods. We quanti ed bacterial community structure as communitylevel physiological pro les and biomass with phospholipid phosphate analysis. Our ndings highlight the worrisome impacts of e uents on microbial biomass and bacterial metabolic diversity on the receiving water. Microbial biomass was signi cantly higher at the WWTP outfall compared to upstream and downstream sites and correlated positively with sediment physicochemical parameters. Furthermore, our data revealed signi cant spatial differences in bacterial community structure in the context of WWTP impact. High nutrient availability (lower carbon/nitrogen ratios) at the outfall increased site-speci c bacterial metabolic diversity in winter but decreased the same in fall. Seasonal changes in the sedimentary microbial biomass and bacterial carbon substrate utilization were evident regardless of the spatial variations or impacts of the wastewater e uents. Communities in fall showed more versatile substrate utilization patterns than the winter communities. These results suggest that WWTP e uents signi cantly increased microbial biomass and highlight its mixed effects on bacterial community structure and metabolic diversity. Also, our data underscore a close association between sedimentary physicochemical parameters and the associated microbial functional activities.

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Shifts of Bacterioplankton Metabolic Profiles along the Salinity Gradient in a Subtropical Estuary

Cited by 12 publications

References 33 publications

Patterns of Change in Metabolic Capabilities of Sediment Microbial Communities in River and Lake Ecosystems

Patterns of Change in Metabolic Capabilities of Sediment Microbial Communities in River and Lake Ecosystems

Bacterial Communities Associated with Four Cyanobacterial Genera Display Structural and Functional Differences: Evidence from an Experimental Approach

Spatio-temporal variations in the physiological profiles of streambed bacterial communities: implication of wastewater treatment plant effluents

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