Shiga toxigenic and enteropathogenic <i>Escherichia coli</i>
 in water and fish from pay-to-fish ponds

Ribeiro, Laryssa Freitas; Barbosa, Mayhara Martins Cordeiro; Pinto, Fernanda de Rezende; Guariz, Cíntia Sobue Lorenzon; Maluta, Renato Pariz; Rossi, Juliana Regina; Rossi, Gabriel Augusto Marques; Lemos, M. V. F.; Amaral, Luiz Augusto do

doi:10.1111/lam.12536

Cited by 25 publications

(30 citation statements)

References 34 publications

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“…The presence of these genes is predominant in patients with Hemolytic Uremic Syndrome. Although the detection of strains carrying both eae and Stx genes in aquaculture environments is low (Zschock et al, 2000;Irino et al, 2005;Alagarsamy et al, 2009), it has been detected in the present work and in approximately 7.69% of intestinal fish strains from ponds in northeast of Sao Paulo (Ribeiro et al, 2016). Invasive gene ial was identified only in gills (3.33%) but ipah invasive gene was not detected in othe present study.…”

Section: Virulence Genes Detected Among E Coli Isolatedcontrasting

confidence: 62%

“…All pathogenic genes identified in this study are likely to cause diarrhea, dysentery, haemorrhagic colitis, haemolytic uremic syndrome and chronic post-infection sequelae in men (Ribeiro et al, 2016). Their detections in gills and viscera of fish could come from direct contact with water (Fouz et al, 2000).…”

Section: Virulence Genes Detected Among E Coli Isolatedmentioning

confidence: 83%

“…The positive isolate for both Stx2 and eae, characteristic of Enterohemoragique E. coli (EHEC) but included in STEC, was identified in gills at 1.66% and in viscera at 1.66% in 1 strain. The presence of Shigatoxigenic genes has also been identified in intestines of fish from ponds in northeast of Sao Paulo, Brazil (Ribeiro et al, 2016). The presence of these genes is predominant in patients with Hemolytic Uremic Syndrome.…”

Section: Virulence Genes Detected Among E Coli Isolatedmentioning

confidence: 96%

“…E. coli is a normal habitant of warm-blooded animals. Although not a normal host of fish flora, it has been isolated from stomach, gut, gills, muscle, and skin of fish (Janssen, 1970;Hejkel et al, 1983;Ogbondeminu, 1993;Kouadio-Ngbesso et al, 2016;Ribeiro et al, 2016). The presence of E. coli in viscera and gills in Layo's fish may be due to the quality of water in which they are cultured.…”

Section: Frequency Of E Coli Strains Isolatedmentioning

confidence: 99%

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Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

̈ge¹,

Mireille²,

RenÃ³

et al. 2019

Afr. J. Microbiol. Res.

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The objective of this study was to know the sanitary quality of fish coming from Layo farm. Twenty fishes (Oreochromis niloticus), were selected in four ponds and Escherichia coli were isolated in gills and viscera according to microbiological methods. One hundred and twenty strains of E. coli were isolated, and their virulence was performed by polymerase chain reaction (PCR) using specific primers. Eleven (11) strains (9.16%) including 7 strains of gills (11.66%) and 4 strains of viscera (6.66%) had virulence genes eae, Stx1, Stx2 or ial. Atypical Enteropathogenic E. coli (EPEC, eae+, lack of bfp) was isolated from gills (5%) and viscera (1.66%). Shiga toxigenic E. coli (STEC) with genes eae + Stx2, Stx1 and Stx2 were isolated in viscera (5%) and gills (3.33%). For Enteroinvasive E. coli (EIEC), ial gene was isolated in gills (3.33%) but no ipah gene. Enterotoxinogen E. coli (ETEC) with lt gene and Enteroaggregating E. coli (EAEC) with aggA gene were not detected in this study. This study revealed that some fish from Layo farm are carriers of virulent E. coli that can cause serious human diseases and can lead to consumer death if cooking is insufficient or by cross-contamination. This therefore poses a real public health problem.

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Section: Virulence Genes Detected Among E Coli Isolatedcontrasting

confidence: 62%

Section: Virulence Genes Detected Among E Coli Isolatedmentioning

confidence: 83%

Section: Virulence Genes Detected Among E Coli Isolatedmentioning

confidence: 96%

Section: Frequency Of E Coli Strains Isolatedmentioning

confidence: 99%

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Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

̈ge¹,

Mireille²,

RenÃ³

et al. 2019

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

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“…The role of different animal species as asymptomatic carriers of EHEC/STEC has been extensively studied in the last years in Brazil. Besides cattle, which are their most common natural reservoir,173, 178 the presence of these pathogens has been identified in the feces of dairy buffaloes, 179 sheep,180, 181 pigs,182, 183 birds, 184 and fishes 185 . It is noteworthy that some relevant serotypes linked to human infections such as O103:H2 and O157:H7 have been recovered from the feces of sheep 186 and cattle 173 respectively.…”

Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

432

353

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Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are collectively known as diarrheagenic E. coli. In this review, we have gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.

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Astaxanthin improves the shelf‐life of tilapia fillets stored under refrigeration

et al. 2022

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BACKGROUND Astaxanthin, classified as a xanthophyll, has antioxidant properties about 500 times greater than α‐tocopherols and ten times greater than β‐carotenes. Based on the antioxidant activity of this carotenoid, this study aimed to evaluate the shelf‐life of tilapia fillets (Oreochromis niloticus) fed with astaxanthin, by determining the microbiological quality (colimetry, counts of mesophilic and psychrotrophic microorganisms), physicochemical analyses (colorimetry, pH, thiobarbituric acid reactive substances (TBARS)) and sensory analysis. RESULTS Tilapia supplemented with astaxanthin presented a reduction in the counts of microorganisms (mesophiles and psychrotrophics) and lower lipid oxidation index (TBARS), when compared to fillets of control fish. Colorimetric changes of fillet degradation were observed, associated with increased pH during storage, as well as loss of brightness and texture in addition to worsening of appearance and odor. These deteriorating changes were minimized using astaxanthin. CONCLUSION Our results demonstrate the beneficial performance of astaxanthin in the shelf‐life of tilapia fillets stored under refrigeration. Therefore, dietary supplementation with astaxanthin (100 and 200 mg kg−1 of feed) improves the microbiological and physicochemical quality of tilapia fillets during 50 days of shelf‐life. © 2022 Society of Chemical Industry.

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Shiga toxigenic and enteropathogenic Escherichia coli in water and fish from pay-to-fish ponds

Cited by 25 publications

References 34 publications

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

Diarrheagenic Escherichia coli

Astaxanthin improves the shelf‐life of tilapia fillets stored under refrigeration

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