Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes): A Species with Antioxidant, Immunomodulatory, and Hepatoprotective Activities in Hypercholesterolemic Rats

Nisar, Jaweria; Mustafa, Imtiaz; Anwar, Haseeb; Sohail, Muhammad Umar; Hussain, Ghulam; Ullah, Muhammad Irfan; Faisal, Muhammad Naeem; Bukhari, Shazia Anwer; Basit, Abdul

doi:10.1615/intjmedmushrooms.2017024504

Cited by 25 publications

(26 citation statements)

References 18 publications

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“…In recent decades, various natural plant extracts have exhibited significant antioxidant activity [1,2]. These extracts encompass significant amounts of different bioactive molecules used in various pharmaceutical industry products [3,4].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

Mustafa

Faisal

Hussain

et al. 2021

BMC Complement Med Ther

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Background Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. Methods The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH● assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase. Results After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH● (IC50 value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe+ 2/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC50 value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC50 value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively). Conclusion The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes.

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Section: Introductionmentioning

confidence: 99%

Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

Mustafa

Faisal

Hussain

et al. 2021

BMC Complement Med Ther

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“…Many studies revealed that plants as well as mushrooms have the antioxidant abilities. 9,10 Acetylsalicylic acid (ASA) is a vital signal molecule concerned within the plants growing processes, flower's enlargement and plants defense mechanism. 11 The Exogenous application of aspirin enhanced the CO 2 assimilation rate plant and increased dry masses under drought stress.…”

Section: Introductionmentioning

confidence: 99%

Foliar Applied Acetylsalicylic Acid Induced Growth and Key-Biochemical Changes in Chickpea (Cicer arietinum L.) Under Drought Stress

et al. 2020

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The exogenous application of acetylsalicylic acid (ASA) is stated to increase tolerance of plants against different environmental stresses. Therefore, the present study was planned to get insight into ASA-mediated regulation of growth, secondary metabolism, and oxidative defense in 2 chickpea varieties. Ten seeds of 2 chickpea varieties (DG-89 and Bittle-98) were sown in plastic pots containing sandy loam soil with 3 drought stress levels, i.e. wet conditions or flooded water (100% FC) as recommended control, 75% FC, 50% FC and 25% FC for chickpea. The moisture contents were maintained and regularly monitored through the addition of normal irrigation water. The design of experimental was completely randomized with 3 replicates per treatment. Penultimate leaves were harvested with knife after 20 days of foliar spray to observe the effect of exogenously applied ASA (100 mg/L) on growth, and key-biochemical attributes of chickpea plants (DG-89 and Bittle-98) under drought stress regimes. Drought stress regimes caused a substantial decline in shoot (37% and 35%) and root length (67% and 78%), shoot (80% and 76%) and root (62% and 68%) fresh masses, shoot (71% and 63%) and root (77% and 74%) dry masses, leaf area per plant (77% and 80%), chlorophyll a (7% and 45%), chlorophyll b (57% and 42%), total chlorophyll (30% and 39%), total carotenoids (76% and 54%), total anthocyanins (38%), reducing sugar (10% and 57%), total soluble proteins (77% and 44%), total flavonoids (61% and 59%) and total phenolics (58% and 31%) contents in both DG-89 and Bittle-98, respectively. A significant increase in MDA (25%), H2O2 contents (100% and 62%), proline (145% and 131%), and ascorbic acid (133% and 203%) contents was documented in stressed plants of both varieties, respectively. Additionally, drought stress significantly improved the activities of POD (154% and 76%), CAT (87% and 45%) and SOD (248% and 143%) in both varieties. Exogenous application of ASA reduced drought-mediated oxidative stress by reducing MDA (53% and 14%), and H2O2 (84% and 56%) contents, proline contents (50% and 17%) and enhanced the shoot (6% and 25%) and root (43% and 33%) dry masses, leaf area (9% and 10%), chlorophyll a (7% and 32%), b (82% and 81%), and carotenoids (53% and 33%) in both barley cultivars. When plants of chickpea was treated with ASA had greater total anthocyanins (26% and 35%), free amino acids (48% and 28%), ascorbic acid contents (135% and 179%), total soluble proteins (34% and 23%), total flavonoids (58% and 35%) and phenolic (50% and 69%)contents besides the POD (41% and 64%), CAT (23% and 56%) and SOD (73% and 72%) enzymes activities. Plants of DG-89 showed more tolerance to drought stress than that of Bittle-98 as a manifest from higher plant biomasses. Thus, our results showed that foliar-applied ASA is an effective strategy that can be used to improve the tolerance of chickpea plants to drought stress.

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“…[40,41] Particularly, probiotic and prebiotic supplementations in T2DM adults that reported increase in Bi dobacterium and Coprococcus counts also showed marked improvement in glucose and lipid homeostasis [42]. Besides, restoration of Bi dobacterium and Coprococcus spp., these supplements were also associated with alleviations of hyperglycemia, hyperlipidemia, and hepatic steatosis in mice fed with HFD [43,44].…”

Section: Discussionmentioning

confidence: 94%

Gut Microbiome Analysis Reveals Characteristics Decrease in Bifidobacterium Count in the Adult Type 2 Diabetes Mellitus Patients

Sattar

Anwar

Sohail³

et al. 2020

Preprint

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Background: Type 2 diabetes mellitus is an idiopathic metabolic disorder in epidemic phase. The current study examines the gut microbiome of diabetic (Diab), diabetic with secondary complications (DSC), and healthy control (HC) subjects enrolled at the district hospital, Faisalabad, Pakistan. The study groups were characterized based on serum glucose and insulin concentrations and the subject's clinical history. Serum IgM, leptin, and amylin concentrations were determined using ELISA. The stool microbiome was analyzed using 16S rRNA amplicon sequencing on the Illumina MiSeq. Results: Serum IgM, leptin, and amylin concentrations were higher (p ≤ 0.05) in the Diab and DSC groups compared with the HC group. Results: No statistical differences were observed in microbiome composition and diversity at community levels as evaluated by alpha (Kruskal–Wallis p = 0.16) and beta (PERMANOVA p = 0.41) diversity indexes. Firmicutes (51.2%), Proteobacteria (14.9%), Actinobacteria (12.5%), and Bacteroidetes (7.8%) were the predominant bacterial phyla present in the stool microbiome of the study population. However, no differences (p ≥ 0.05) in phylum-level taxonomic composition were observed between the study groups. At genus level, Coprococcus and Ruminococcus from phylum Firmicutes and Bifidobacterium from phylum Actinobacteria were significantly different (p ≤ 0.05) between the study groups. Bifidobacterium and Coprococcus were higher in the HC group, whereas Ruminococcus was higher in the DSC group. Conclusions: Taken together, in the present study, we report the gut microbiome of diabetic patients, which has specific taxonomic characteristics associated with the incidence and progression of diabetes.

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Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes): A Species with Antioxidant, Immunomodulatory, and Hepatoprotective Activities in Hypercholesterolemic Rats

Cited by 25 publications

References 18 publications

Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

Foliar Applied Acetylsalicylic Acid Induced Growth and Key-Biochemical Changes in Chickpea (Cicer arietinum L.) Under Drought Stress

Gut Microbiome Analysis Reveals Characteristics Decrease in Bifidobacterium Count in the Adult Type 2 Diabetes Mellitus Patients

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