Shikimic acid biosynthesis in microorganisms: Current status and future direction

Sheng, Qi; Yi, Lingxin; Zhong, Bin; Wu, Xiaoyu; Li, Liming; Zhang, Bin

doi:10.1016/j.biotechadv.2022.108073

Cited by 23 publications

(10 citation statements)

References 157 publications

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“…The biological activities of the AC extract that have been examined may be attributed to the incredibly high concentration of shikimic acid [ 23 ]. Furthermore, salicylic acid, protocatechuic acid, and polydatine were found as important chemicals that may be responsible for their biological features.…”

Section: Resultsmentioning

confidence: 99%

The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents

et al. 2023

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The current work’s main objective was to determine the chemical composition of Amygdalus communis (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure. The EDX spectrum confirmed the formation of AuNPs and a negative zeta potential value (−27.7 mV) suggested their physicochemical stability. The in vitro cytotoxic efficacy of the AC-AuNPs against colorectal adenocarcinoma (Caco-2), glioma (U118), and ovarian (Skov-3) cancer cell lines and human dermal fibroblasts (HDFs) was evaluated by MTT assay. CaCo-2 cell proliferation was effectively inhibited by the AC-AuNPs at concentrations between 25 and 100 g mL−1. The AC-AuNPs exerted preeminent antimicrobial activity against Bacillus subtilis with an MIC of 0.02 μg/mL, whilst good activity was shown against Staphylococcus aureus bacteria and Candida albicans yeast with an MIC of 0.12 μg/mL. Ultimately, the results support the high antibacterial and anticancer potential of biosynthesized AuNPs from AC leaf extract.

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Section: Resultsmentioning

confidence: 99%

The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents

et al. 2023

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“…The bifunctional regulation system was implemented to modulate the biosynthesis of SA, a crucial intermediate in the production of aromatic amino acids . To increase the production of SA, it becomes imperative to manipulate the glucose transport system and glycolysis process for increasing the supply of precursors. − In addition, the accumulation of SA can be facilitated by preventing further downstream conversion into aromatic amino acids. , The bifunctional regulation system was utilized to inhibit the expression of genes hdpA , aroK, and pyk in the wild-type C. glutamicum ATCC13032, thereby minimizing the diversion of precursor phosphoenolpyruvate (PEP) during glucose transportation and reducing SA consumption.…”

Section: Resultsmentioning

confidence: 99%

“…42−44 In addition, the accumulation of SA can be facilitated by preventing further downstream conversion into aromatic amino acids. 43,44 The bifunctional regulation system was utilized to inhibit the expression of genes hdpA, aroK, and pyk in the wild-type C. glutamicum ATCC13032, thereby minimizing the diversion of precursor phosphoenolpyruvate (PEP) during glucose transportation and reducing SA consumption. Furthermore, this system was employed to activate the expression of genes ptsH, iolP, and tkt, leading to an improved supply of glucose and an enhanced accumulation of precursor erythrose 4-phosphate (E4P).…”

Section: Metabolic Pathwaymentioning

confidence: 99%

Rewiring Metabolic Flux in Corynebacterium glutamicum Using a CRISPR/dCpf1-Based Bifunctional Regulation System

Yin,

Xi,

Shen

et al. 2024

J. Agric. Food Chem.

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Corynebacterium glutamicum, a microorganism classified as generally recognized as safe for use in the industrial production of food raw materials and additives, has encountered challenges in achieving widespread adoption and popularization as microbial cell factories. These obstacles arise from the intricate nature of manipulating metabolic flux through conventional methods, such as gene knockout and enzyme overexpression. To address this challenge, we developed a CRISPR/dCpf1-based bifunctional regulation system to bidirectionally regulate the expression of multiple genes in C. glutamicum. Specifically, through fusing various transcription factors to the C-terminus of dCpf1, the resulting dCpf1-SoxS exhibited both CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) capabilities in C. glutamicum by altering the binding sites of crRNAs. The bifunctional regulation system was used to fine-tune metabolic flux from shikimic acid (SA) and L-serine biosynthesis, resulting in 27-fold and 10-fold increases in SA and L-serine production, respectively, compared to the original strain. These findings highlight the potential of the CRISPR/dCpf1-based bifunctional regulation system in effectively enhancing the yield of target products in C. glutamicum.

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“…ROS oxidize amino acids and the alteration of this key organic component result in loss of given protein-mediated functions including metabolic, structural, transport, and regulatory activities, which ultimately leads to cell death in plants [2,90,91]. Three essential amino acids-tryptophan, tyrosine, and phenylalanine-produced by the shikimate pathway are essential for plant development, stress tolerance, and pest resistance [92]. Studies have demonstrated that, under water stress, maize and chickpea plants hyper-accumulated tryptophan, phenylalanine, proline, and tyrosine [83,93].…”

Section: Discussionmentioning

confidence: 99%

Biochar-Mediated Control of Metabolites and Other Physiological Responses in Water-Stressed Leptocohloa fusca

et al. 2023

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We investigated biochar-induced drought tolerance in Leptocohloa fusca (Kallar grass) by exploring the plant defense system at physiological level. L. fusca plants were exposed to drought stress (100%, 70%, and 30% field capacity), and biochar (BC), as an organic soil amendment was applied in two concentrations (15 and 30 mg kg−1 soil) to induce drought tolerance. Our results demonstrated that drought restricted the growth of L. fusca by inhibiting shoot and root (fresh and dry) weight, total chlorophyll content and photosynthetic rate. Under drought stress, the uptake of essential nutrients was also limited due to lower water supply, which ultimately affected metabolites including amino and organic acids, and soluble sugars. In addition, drought stress induced oxidative stress, which is evidenced by the higher production of reactive oxygen species (ROS) including hydrogen peroxide (H2O2), superoxide ion (O2−), hydroxyl ion (OH−), and malondialdehyde (MDA). The current study revealed that stress-induced oxidative injury is not a linear path, since the excessive production of lipid peroxidation led to the accumulation of methylglyoxal (MG), a member of reactive carbonyl species (RCS), which ultimately caused cell injury. As a consequence of oxidative-stress induction, the ascorbate–glutathione (AsA–GSH) pathway, followed by a series of reactions, was activated by the plants to reduce ROS-induced oxidative damage. Furthermore, biochar considerably improved plant growth and development by mediating metabolites and soil physio-chemical status.

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Shikimic acid biosynthesis in microorganisms: Current status and future direction

Cited by 23 publications

References 157 publications

The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents

The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents

Rewiring Metabolic Flux in Corynebacterium glutamicum Using a CRISPR/dCpf1-Based Bifunctional Regulation System

Biochar-Mediated Control of Metabolites and Other Physiological Responses in Water-Stressed Leptocohloa fusca

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